Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14730 and BPHYT_RS14735 are separated by 1 nucleotides BPHYT_RS14735 and BPHYT_RS14740 are separated by 201 nucleotides BPHYT_RS14740 and BPHYT_RS14745 are separated by 113 nucleotides BPHYT_RS14745 and BPHYT_RS14750 are separated by 93 nucleotides
BPHYT_RS14730: BPHYT_RS14730 - GTPase RsgA, at 3,354,981 to 3,355,931
_RS14730
BPHYT_RS14735: BPHYT_RS14735 - hypothetical protein, at 3,355,933 to 3,356,253
_RS14735
BPHYT_RS14740: BPHYT_RS14740 - threonine-phosphate decarboxylase, at 3,356,455 to 3,357,393
_RS14740
BPHYT_RS14745: BPHYT_RS14745 - DNA mismatch repair protein MutT, at 3,357,507 to 3,358,235
_RS14745
BPHYT_RS14750: BPHYT_RS14750 - 50S ribosomal protein L19, at 3,358,329 to 3,358,718
_RS14750
Position (kb)
3356
3357
3358 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3355.890 kb on + strand at 3356.205 kb on + strand, within BPHYT_RS14735 at 3356.283 kb on + strand at 3356.284 kb on - strand at 3356.506 kb on + strand at 3356.507 kb on - strand at 3356.726 kb on + strand, within BPHYT_RS14740 at 3356.727 kb on - strand, within BPHYT_RS14740 at 3356.986 kb on - strand, within BPHYT_RS14740 at 3356.986 kb on - strand, within BPHYT_RS14740 at 3356.986 kb on - strand, within BPHYT_RS14740 at 3357.013 kb on - strand, within BPHYT_RS14740 at 3357.021 kb on + strand, within BPHYT_RS14740 at 3357.137 kb on + strand, within BPHYT_RS14740 at 3357.186 kb on - strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.194 kb on + strand, within BPHYT_RS14740 at 3357.195 kb on - strand, within BPHYT_RS14740 at 3357.195 kb on - strand, within BPHYT_RS14740 at 3357.195 kb on - strand, within BPHYT_RS14740 at 3357.197 kb on + strand, within BPHYT_RS14740 at 3357.197 kb on + strand, within BPHYT_RS14740 at 3357.305 kb on + strand at 3357.376 kb on + strand at 3357.419 kb on + strand at 3357.420 kb on - strand at 3357.430 kb on + strand at 3357.430 kb on + strand at 3357.431 kb on - strand at 3357.526 kb on + strand at 3357.568 kb on + strand at 3357.569 kb on - strand at 3358.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,355,890 + -0.8 3,356,205 + BPHYT_RS14735 0.85 -0.4 3,356,283 + -0.9 3,356,284 - -1.6 3,356,506 + -1.6 3,356,507 - +1.4 3,356,726 + BPHYT_RS14740 0.29 +2.2 3,356,727 - BPHYT_RS14740 0.29 +0.4 3,356,986 - BPHYT_RS14740 0.57 +0.9 3,356,986 - BPHYT_RS14740 0.57 +0.8 3,356,986 - BPHYT_RS14740 0.57 +0.7 3,357,013 - BPHYT_RS14740 0.59 +0.6 3,357,021 + BPHYT_RS14740 0.60 +1.0 3,357,137 + BPHYT_RS14740 0.73 +1.0 3,357,186 - BPHYT_RS14740 0.78 +0.4 3,357,194 + BPHYT_RS14740 0.79 +1.3 3,357,194 + BPHYT_RS14740 0.79 -0.3 3,357,194 + BPHYT_RS14740 0.79 +1.3 3,357,194 + BPHYT_RS14740 0.79 +1.9 3,357,194 + BPHYT_RS14740 0.79 +1.2 3,357,195 - BPHYT_RS14740 0.79 +1.6 3,357,195 - BPHYT_RS14740 0.79 +0.8 3,357,195 - BPHYT_RS14740 0.79 +1.3 3,357,197 + BPHYT_RS14740 0.79 +1.1 3,357,197 + BPHYT_RS14740 0.79 +1.2 3,357,305 + +0.8 3,357,376 + +0.2 3,357,419 + +0.6 3,357,420 - -1.8 3,357,430 + +1.6 3,357,430 + +1.5 3,357,431 - +0.3 3,357,526 + -1.1 3,357,568 + -0.0 3,357,569 - +0.0 3,358,321 + -1.0
Or see this region's nucleotide sequence