Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14120 and BPHYT_RS14125 are separated by 26 nucleotides BPHYT_RS14125 and BPHYT_RS14130 are separated by 28 nucleotides BPHYT_RS14130 and BPHYT_RS14135 are separated by 17 nucleotides BPHYT_RS14135 and BPHYT_RS14140 overlap by 4 nucleotides
BPHYT_RS14120: BPHYT_RS14120 - chemotaxis protein CheY, at 3,225,241 to 3,225,942
_RS14120
BPHYT_RS14125: BPHYT_RS14125 - transcriptional regulator, at 3,225,969 to 3,226,673
_RS14125
BPHYT_RS14130: BPHYT_RS14130 - phosphate ABC transporter ATP-binding protein, at 3,226,702 to 3,227,550
_RS14130
BPHYT_RS14135: BPHYT_RS14135 - phosphate transporter permease subunit PtsA, at 3,227,568 to 3,228,464
_RS14135
BPHYT_RS14140: BPHYT_RS14140 - phosphate transporter permease subunit PstC, at 3,228,461 to 3,229,462
_RS14140
Position (kb)
3226
3227
3228 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3225.714 kb on + strand, within BPHYT_RS14120 at 3225.845 kb on + strand, within BPHYT_RS14120 at 3225.846 kb on - strand, within BPHYT_RS14120 at 3225.971 kb on - strand at 3226.036 kb on + strand at 3226.147 kb on + strand, within BPHYT_RS14125 at 3226.148 kb on - strand, within BPHYT_RS14125 at 3226.679 kb on - strand at 3226.679 kb on - strand at 3226.688 kb on + strand at 3226.688 kb on + strand at 3226.688 kb on + strand at 3226.688 kb on + strand at 3226.689 kb on - strand at 3226.724 kb on + strand at 3226.724 kb on + strand at 3226.799 kb on + strand, within BPHYT_RS14130 at 3226.799 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.805 kb on + strand, within BPHYT_RS14130 at 3226.805 kb on + strand, within BPHYT_RS14130 at 3226.814 kb on + strand, within BPHYT_RS14130 at 3226.868 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.408 kb on + strand, within BPHYT_RS14130 at 3227.544 kb on + strand at 3227.569 kb on + strand at 3227.570 kb on - strand at 3227.570 kb on - strand at 3227.689 kb on + strand, within BPHYT_RS14135 at 3227.689 kb on + strand, within BPHYT_RS14135 at 3228.300 kb on - strand, within BPHYT_RS14135 at 3228.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,225,714 + BPHYT_RS14120 0.67 -0.0 3,225,845 + BPHYT_RS14120 0.86 +0.2 3,225,846 - BPHYT_RS14120 0.86 +1.5 3,225,971 - -2.0 3,226,036 + +0.1 3,226,147 + BPHYT_RS14125 0.25 +0.6 3,226,148 - BPHYT_RS14125 0.25 -1.8 3,226,679 - -1.1 3,226,679 - +0.1 3,226,688 + +0.6 3,226,688 + +0.6 3,226,688 + +0.9 3,226,688 + -2.0 3,226,689 - -0.2 3,226,724 + +0.1 3,226,724 + +0.0 3,226,799 + BPHYT_RS14130 0.11 +0.6 3,226,799 + BPHYT_RS14130 0.11 +0.6 3,226,803 + BPHYT_RS14130 0.12 +1.2 3,226,803 + BPHYT_RS14130 0.12 -0.1 3,226,803 + BPHYT_RS14130 0.12 -1.4 3,226,803 + BPHYT_RS14130 0.12 +0.6 3,226,803 + BPHYT_RS14130 0.12 +1.0 3,226,805 + BPHYT_RS14130 0.12 +2.8 3,226,805 + BPHYT_RS14130 0.12 +1.4 3,226,814 + BPHYT_RS14130 0.13 +3.0 3,226,868 + BPHYT_RS14130 0.20 +1.7 3,227,297 + BPHYT_RS14130 0.70 +1.5 3,227,297 + BPHYT_RS14130 0.70 +1.7 3,227,297 + BPHYT_RS14130 0.70 +2.0 3,227,297 + BPHYT_RS14130 0.70 -4.9 3,227,408 + BPHYT_RS14130 0.83 +1.9 3,227,544 + -0.1 3,227,569 + +2.6 3,227,570 - +0.3 3,227,570 - -0.2 3,227,689 + BPHYT_RS14135 0.13 +1.6 3,227,689 + BPHYT_RS14135 0.13 +1.9 3,228,300 - BPHYT_RS14135 0.82 +3.4 3,228,444 - +3.6
Or see this region's nucleotide sequence