Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13645 and BPHYT_RS13650 are separated by 252 nucleotides BPHYT_RS13650 and BPHYT_RS13655 are separated by 131 nucleotides
BPHYT_RS13645: BPHYT_RS13645 - threonine--tRNA ligase, at 3,109,420 to 3,111,327
_RS13645
BPHYT_RS13650: BPHYT_RS13650 - tRNA-Val, at 3,111,580 to 3,111,656
_RS13650
BPHYT_RS13655: BPHYT_RS13655 - GTP pyrophosphokinase, at 3,111,788 to 3,114,031
_RS13655
Position (kb)
3111
3112 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3110.636 kb on + strand, within BPHYT_RS13645 at 3110.637 kb on - strand, within BPHYT_RS13645 at 3110.637 kb on - strand, within BPHYT_RS13645 at 3110.637 kb on - strand, within BPHYT_RS13645 at 3110.675 kb on + strand, within BPHYT_RS13645 at 3110.675 kb on + strand, within BPHYT_RS13645 at 3110.676 kb on - strand, within BPHYT_RS13645 at 3110.813 kb on + strand, within BPHYT_RS13645 at 3110.813 kb on + strand, within BPHYT_RS13645 at 3110.813 kb on + strand, within BPHYT_RS13645 at 3110.813 kb on + strand, within BPHYT_RS13645 at 3110.814 kb on - strand, within BPHYT_RS13645 at 3110.814 kb on - strand, within BPHYT_RS13645 at 3110.862 kb on + strand, within BPHYT_RS13645 at 3110.864 kb on + strand, within BPHYT_RS13645 at 3110.864 kb on + strand, within BPHYT_RS13645 at 3110.865 kb on - strand, within BPHYT_RS13645 at 3110.865 kb on - strand, within BPHYT_RS13645 at 3110.865 kb on - strand, within BPHYT_RS13645 at 3110.889 kb on - strand, within BPHYT_RS13645 at 3111.006 kb on - strand, within BPHYT_RS13645 at 3111.006 kb on - strand, within BPHYT_RS13645 at 3111.017 kb on + strand, within BPHYT_RS13645 at 3111.017 kb on + strand, within BPHYT_RS13645 at 3111.018 kb on - strand, within BPHYT_RS13645 at 3111.020 kb on + strand, within BPHYT_RS13645 at 3111.020 kb on + strand, within BPHYT_RS13645 at 3111.021 kb on - strand, within BPHYT_RS13645 at 3111.021 kb on - strand, within BPHYT_RS13645 at 3111.086 kb on + strand, within BPHYT_RS13645 at 3111.086 kb on + strand, within BPHYT_RS13645 at 3111.189 kb on + strand at 3111.288 kb on - strand at 3111.288 kb on - strand at 3111.316 kb on + strand at 3111.316 kb on + strand at 3111.317 kb on - strand at 3111.362 kb on + strand at 3111.362 kb on + strand at 3111.363 kb on - strand at 3111.363 kb on - strand at 3111.363 kb on - strand at 3111.363 kb on - strand at 3111.363 kb on - strand at 3111.363 kb on - strand at 3111.434 kb on + strand at 3111.476 kb on - strand at 3111.509 kb on - strand at 3111.534 kb on + strand at 3111.671 kb on - strand at 3111.671 kb on - strand at 3111.671 kb on - strand at 3111.671 kb on - strand at 3111.698 kb on + strand at 3111.705 kb on - strand at 3111.754 kb on + strand at 3111.754 kb on + strand at 3111.754 kb on + strand at 3111.755 kb on - strand at 3111.839 kb on - strand at 3111.839 kb on - strand at 3111.964 kb on + strand at 3112.017 kb on + strand, within BPHYT_RS13655 at 3112.165 kb on + strand, within BPHYT_RS13655 at 3112.276 kb on + strand, within BPHYT_RS13655 at 3112.277 kb on - strand, within BPHYT_RS13655 at 3112.277 kb on - strand, within BPHYT_RS13655 at 3112.499 kb on - strand, within BPHYT_RS13655
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,110,636 + BPHYT_RS13645 0.64 +1.9 3,110,637 - BPHYT_RS13645 0.64 +2.2 3,110,637 - BPHYT_RS13645 0.64 +2.9 3,110,637 - BPHYT_RS13645 0.64 +2.1 3,110,675 + BPHYT_RS13645 0.66 +2.2 3,110,675 + BPHYT_RS13645 0.66 +2.7 3,110,676 - BPHYT_RS13645 0.66 +1.9 3,110,813 + BPHYT_RS13645 0.73 +3.1 3,110,813 + BPHYT_RS13645 0.73 +3.9 3,110,813 + BPHYT_RS13645 0.73 +3.3 3,110,813 + BPHYT_RS13645 0.73 +2.1 3,110,814 - BPHYT_RS13645 0.73 +1.8 3,110,814 - BPHYT_RS13645 0.73 +3.2 3,110,862 + BPHYT_RS13645 0.76 +3.0 3,110,864 + BPHYT_RS13645 0.76 +3.1 3,110,864 + BPHYT_RS13645 0.76 +2.7 3,110,865 - BPHYT_RS13645 0.76 +2.8 3,110,865 - BPHYT_RS13645 0.76 +3.3 3,110,865 - BPHYT_RS13645 0.76 +2.8 3,110,889 - BPHYT_RS13645 0.77 +2.8 3,111,006 - BPHYT_RS13645 0.83 +2.9 3,111,006 - BPHYT_RS13645 0.83 +3.8 3,111,017 + BPHYT_RS13645 0.84 +1.7 3,111,017 + BPHYT_RS13645 0.84 +2.8 3,111,018 - BPHYT_RS13645 0.84 +2.5 3,111,020 + BPHYT_RS13645 0.84 +2.8 3,111,020 + BPHYT_RS13645 0.84 +3.3 3,111,021 - BPHYT_RS13645 0.84 +4.0 3,111,021 - BPHYT_RS13645 0.84 +3.2 3,111,086 + BPHYT_RS13645 0.87 +4.6 3,111,086 + BPHYT_RS13645 0.87 +2.7 3,111,189 + +2.3 3,111,288 - +2.4 3,111,288 - +1.6 3,111,316 + +3.4 3,111,316 + +1.2 3,111,317 - +3.0 3,111,362 + +2.0 3,111,362 + +2.7 3,111,363 - +1.5 3,111,363 - +2.9 3,111,363 - -0.8 3,111,363 - +2.1 3,111,363 - +1.7 3,111,363 - +0.6 3,111,434 + +2.7 3,111,476 - -0.9 3,111,509 - -1.0 3,111,534 + +2.5 3,111,671 - +0.5 3,111,671 - +1.1 3,111,671 - +1.7 3,111,671 - +0.4 3,111,698 + +0.0 3,111,705 - +0.2 3,111,754 + +0.6 3,111,754 + -0.3 3,111,754 + +1.3 3,111,755 - -0.7 3,111,839 - -0.5 3,111,839 - -1.9 3,111,964 + +0.7 3,112,017 + BPHYT_RS13655 0.10 -0.2 3,112,165 + BPHYT_RS13655 0.17 -1.1 3,112,276 + BPHYT_RS13655 0.22 -2.2 3,112,277 - BPHYT_RS13655 0.22 -0.2 3,112,277 - BPHYT_RS13655 0.22 -0.5 3,112,499 - BPHYT_RS13655 0.32 -0.5
Or see this region's nucleotide sequence