Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13065 and BPHYT_RS13070 are separated by 169 nucleotides BPHYT_RS13070 and BPHYT_RS13075 are separated by 3 nucleotides BPHYT_RS13075 and BPHYT_RS13080 are separated by 19 nucleotides
BPHYT_RS13065: BPHYT_RS13065 - 2-aminoadipate aminotransferase, at 2,985,745 to 2,987,178
_RS13065
BPHYT_RS13070: BPHYT_RS13070 - oxidoreductase molybdopterin subunit, at 2,987,348 to 2,989,663
_RS13070
BPHYT_RS13075: BPHYT_RS13075 - hypothetical protein, at 2,989,667 to 2,990,170
_RS13075
BPHYT_RS13080: BPHYT_RS13080 - hypothetical protein, at 2,990,190 to 2,990,822
_RS13080
Position (kb)
2987
2988
2989
2990 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2986.547 kb on + strand, within BPHYT_RS13065 at 2986.547 kb on + strand, within BPHYT_RS13065 at 2986.551 kb on - strand, within BPHYT_RS13065 at 2986.551 kb on - strand, within BPHYT_RS13065 at 2986.679 kb on + strand, within BPHYT_RS13065 at 2986.679 kb on + strand, within BPHYT_RS13065 at 2987.039 kb on + strand at 2987.040 kb on - strand at 2987.061 kb on + strand at 2987.061 kb on + strand at 2987.292 kb on - strand at 2987.303 kb on + strand at 2987.379 kb on - strand at 2987.379 kb on - strand at 2987.414 kb on + strand at 2987.414 kb on + strand at 2987.414 kb on + strand at 2987.627 kb on - strand, within BPHYT_RS13070 at 2987.627 kb on - strand, within BPHYT_RS13070 at 2987.804 kb on + strand, within BPHYT_RS13070 at 2987.804 kb on + strand, within BPHYT_RS13070 at 2987.843 kb on + strand, within BPHYT_RS13070 at 2987.864 kb on + strand, within BPHYT_RS13070 at 2987.865 kb on - strand, within BPHYT_RS13070 at 2988.310 kb on + strand, within BPHYT_RS13070 at 2988.310 kb on + strand, within BPHYT_RS13070 at 2988.419 kb on + strand, within BPHYT_RS13070 at 2988.419 kb on + strand, within BPHYT_RS13070 at 2988.420 kb on - strand, within BPHYT_RS13070 at 2988.820 kb on + strand, within BPHYT_RS13070 at 2988.961 kb on + strand, within BPHYT_RS13070 at 2988.961 kb on + strand, within BPHYT_RS13070 at 2989.136 kb on + strand, within BPHYT_RS13070 at 2989.328 kb on + strand, within BPHYT_RS13070 at 2989.328 kb on + strand at 2989.705 kb on - strand at 2989.928 kb on + strand, within BPHYT_RS13075 at 2989.962 kb on + strand, within BPHYT_RS13075 at 2990.023 kb on - strand, within BPHYT_RS13075 at 2990.023 kb on - strand, within BPHYT_RS13075 at 2990.082 kb on + strand, within BPHYT_RS13075 at 2990.413 kb on + strand, within BPHYT_RS13080 at 2990.417 kb on - strand, within BPHYT_RS13080
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,986,547 + BPHYT_RS13065 0.56 -0.1 2,986,547 + BPHYT_RS13065 0.56 +0.6 2,986,551 - BPHYT_RS13065 0.56 +0.2 2,986,551 - BPHYT_RS13065 0.56 +0.0 2,986,679 + BPHYT_RS13065 0.65 -0.3 2,986,679 + BPHYT_RS13065 0.65 -0.4 2,987,039 + -0.3 2,987,040 - -0.3 2,987,061 + +0.3 2,987,061 + -0.6 2,987,292 - -0.2 2,987,303 + -0.5 2,987,379 - -1.4 2,987,379 - -1.4 2,987,414 + +0.8 2,987,414 + +0.2 2,987,414 + +1.0 2,987,627 - BPHYT_RS13070 0.12 -0.6 2,987,627 - BPHYT_RS13070 0.12 +0.5 2,987,804 + BPHYT_RS13070 0.20 +0.1 2,987,804 + BPHYT_RS13070 0.20 +0.2 2,987,843 + BPHYT_RS13070 0.21 -0.6 2,987,864 + BPHYT_RS13070 0.22 -1.1 2,987,865 - BPHYT_RS13070 0.22 -0.1 2,988,310 + BPHYT_RS13070 0.42 +0.7 2,988,310 + BPHYT_RS13070 0.42 +0.1 2,988,419 + BPHYT_RS13070 0.46 -1.4 2,988,419 + BPHYT_RS13070 0.46 -0.4 2,988,420 - BPHYT_RS13070 0.46 -1.2 2,988,820 + BPHYT_RS13070 0.64 -0.4 2,988,961 + BPHYT_RS13070 0.70 -0.2 2,988,961 + BPHYT_RS13070 0.70 -0.4 2,989,136 + BPHYT_RS13070 0.77 +1.3 2,989,328 + BPHYT_RS13070 0.85 +0.6 2,989,328 + +1.2 2,989,705 - +0.8 2,989,928 + BPHYT_RS13075 0.52 -0.0 2,989,962 + BPHYT_RS13075 0.59 -0.2 2,990,023 - BPHYT_RS13075 0.71 -0.2 2,990,023 - BPHYT_RS13075 0.71 -2.2 2,990,082 + BPHYT_RS13075 0.82 +0.6 2,990,413 + BPHYT_RS13080 0.35 +2.1 2,990,417 - BPHYT_RS13080 0.36 +0.5
Or see this region's nucleotide sequence