Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12455

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12445 and BPHYT_RS12450 are separated by 48 nucleotidesBPHYT_RS12450 and BPHYT_RS12455 are separated by 147 nucleotidesBPHYT_RS12455 and BPHYT_RS12460 are separated by 208 nucleotides BPHYT_RS12445: BPHYT_RS12445 - cyclophilin, at 2,837,327 to 2,837,821 _RS12445 BPHYT_RS12450: BPHYT_RS12450 - UDP-2,3-diacylglucosamine hydrolase, at 2,837,870 to 2,838,667 _RS12450 BPHYT_RS12455: BPHYT_RS12455 - serine O-acetyltransferase, at 2,838,815 to 2,839,651 _RS12455 BPHYT_RS12460: BPHYT_RS12460 - RNA methyltransferase, at 2,839,860 to 2,840,693 _RS12460 Position (kb) 2838 2839 2840Strain fitness (log2 ratio) -1 0 1 2at 2837.819 kb on + strandat 2837.819 kb on + strandat 2838.810 kb on - strandat 2838.810 kb on - strandat 2838.961 kb on + strand, within BPHYT_RS12455at 2838.962 kb on - strand, within BPHYT_RS12455at 2839.350 kb on + strand, within BPHYT_RS12455at 2839.350 kb on + strand, within BPHYT_RS12455at 2839.566 kb on + strandat 2839.742 kb on + strandat 2839.745 kb on - strandat 2839.763 kb on + strandat 2840.155 kb on + strand, within BPHYT_RS12460at 2840.155 kb on + strand, within BPHYT_RS12460at 2840.155 kb on + strand, within BPHYT_RS12460at 2840.155 kb on + strand, within BPHYT_RS12460at 2840.155 kb on + strand, within BPHYT_RS12460at 2840.155 kb on + strand, within BPHYT_RS12460at 2840.156 kb on - strand, within BPHYT_RS12460at 2840.214 kb on - strand, within BPHYT_RS12460at 2840.215 kb on + strand, within BPHYT_RS12460at 2840.235 kb on - strand, within BPHYT_RS12460

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,837,819 + -0.0
2,837,819 + +1.2
2,838,810 - -0.2
2,838,810 - +0.3
2,838,961 + BPHYT_RS12455 0.17 -0.7
2,838,962 - BPHYT_RS12455 0.18 -0.1
2,839,350 + BPHYT_RS12455 0.64 -0.8
2,839,350 + BPHYT_RS12455 0.64 +0.0
2,839,566 + +0.5
2,839,742 + +0.6
2,839,745 - -0.6
2,839,763 + +0.1
2,840,155 + BPHYT_RS12460 0.35 +0.3
2,840,155 + BPHYT_RS12460 0.35 +0.2
2,840,155 + BPHYT_RS12460 0.35 +0.7
2,840,155 + BPHYT_RS12460 0.35 -0.5
2,840,155 + BPHYT_RS12460 0.35 +0.5
2,840,155 + BPHYT_RS12460 0.35 +0.8
2,840,156 - BPHYT_RS12460 0.35 -0.1
2,840,214 - BPHYT_RS12460 0.42 +2.3
2,840,215 + BPHYT_RS12460 0.43 +1.1
2,840,235 - BPHYT_RS12460 0.45 -0.4

Or see this region's nucleotide sequence