Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12205 and BPHYT_RS12210 are separated by 392 nucleotides BPHYT_RS12210 and BPHYT_RS12215 are separated by 11 nucleotides BPHYT_RS12215 and BPHYT_RS12220 are separated by 134 nucleotides
BPHYT_RS12205: BPHYT_RS12205 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,786,630 to 2,787,514
_RS12205
BPHYT_RS12210: BPHYT_RS12210 - cardiolipin synthetase, at 2,787,907 to 2,789,358
_RS12210
BPHYT_RS12215: BPHYT_RS12215 - glutathione peroxidase, at 2,789,370 to 2,789,849
_RS12215
BPHYT_RS12220: BPHYT_RS12220 - glutathione ABC transporter ATP-binding protein, at 2,789,984 to 2,791,936
_RS12220
Position (kb)
2787
2788
2789
2790 Strain fitness (log2 ratio)
-1
0
1
2 at 2787.146 kb on + strand, within BPHYT_RS12205 at 2787.147 kb on - strand, within BPHYT_RS12205 at 2787.240 kb on - strand, within BPHYT_RS12205 at 2787.296 kb on - strand, within BPHYT_RS12205 at 2788.048 kb on + strand at 2788.049 kb on - strand at 2788.049 kb on - strand at 2788.066 kb on + strand, within BPHYT_RS12210 at 2788.066 kb on + strand, within BPHYT_RS12210 at 2788.066 kb on + strand, within BPHYT_RS12210 at 2788.305 kb on + strand, within BPHYT_RS12210 at 2788.432 kb on + strand, within BPHYT_RS12210 at 2788.478 kb on - strand, within BPHYT_RS12210 at 2788.576 kb on - strand, within BPHYT_RS12210 at 2788.576 kb on - strand, within BPHYT_RS12210 at 2788.576 kb on - strand, within BPHYT_RS12210 at 2788.636 kb on + strand, within BPHYT_RS12210 at 2788.637 kb on - strand, within BPHYT_RS12210 at 2788.637 kb on - strand, within BPHYT_RS12210 at 2789.047 kb on + strand, within BPHYT_RS12210 at 2789.048 kb on - strand, within BPHYT_RS12210 at 2789.053 kb on + strand, within BPHYT_RS12210 at 2789.054 kb on - strand, within BPHYT_RS12210 at 2789.372 kb on - strand at 2789.425 kb on + strand, within BPHYT_RS12215 at 2789.425 kb on + strand, within BPHYT_RS12215 at 2789.695 kb on + strand, within BPHYT_RS12215 at 2789.695 kb on + strand, within BPHYT_RS12215 at 2789.696 kb on - strand, within BPHYT_RS12215 at 2789.696 kb on - strand, within BPHYT_RS12215 at 2789.704 kb on + strand, within BPHYT_RS12215 at 2789.704 kb on + strand, within BPHYT_RS12215 at 2789.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,787,146 + BPHYT_RS12205 0.58 +0.2 2,787,147 - BPHYT_RS12205 0.58 -1.1 2,787,240 - BPHYT_RS12205 0.69 -0.1 2,787,296 - BPHYT_RS12205 0.75 +0.8 2,788,048 + +0.0 2,788,049 - -0.6 2,788,049 - +1.9 2,788,066 + BPHYT_RS12210 0.11 +1.0 2,788,066 + BPHYT_RS12210 0.11 +0.0 2,788,066 + BPHYT_RS12210 0.11 +0.2 2,788,305 + BPHYT_RS12210 0.27 -0.2 2,788,432 + BPHYT_RS12210 0.36 -0.9 2,788,478 - BPHYT_RS12210 0.39 +0.3 2,788,576 - BPHYT_RS12210 0.46 -0.8 2,788,576 - BPHYT_RS12210 0.46 -0.4 2,788,576 - BPHYT_RS12210 0.46 -0.5 2,788,636 + BPHYT_RS12210 0.50 -0.4 2,788,637 - BPHYT_RS12210 0.50 -0.0 2,788,637 - BPHYT_RS12210 0.50 +0.3 2,789,047 + BPHYT_RS12210 0.79 -0.8 2,789,048 - BPHYT_RS12210 0.79 +0.4 2,789,053 + BPHYT_RS12210 0.79 -0.2 2,789,054 - BPHYT_RS12210 0.79 +0.3 2,789,372 - -0.8 2,789,425 + BPHYT_RS12215 0.11 -0.2 2,789,425 + BPHYT_RS12215 0.11 -0.7 2,789,695 + BPHYT_RS12215 0.68 +2.3 2,789,695 + BPHYT_RS12215 0.68 +0.2 2,789,696 - BPHYT_RS12215 0.68 -0.5 2,789,696 - BPHYT_RS12215 0.68 +0.9 2,789,704 + BPHYT_RS12215 0.70 +0.0 2,789,704 + BPHYT_RS12215 0.70 -1.4 2,789,786 - -0.4
Or see this region's nucleotide sequence