Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11980

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11975 and BPHYT_RS11980 are separated by 701 nucleotidesBPHYT_RS11980 and BPHYT_RS11985 are separated by 131 nucleotides BPHYT_RS11975: BPHYT_RS11975 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 2,724,731 to 2,725,939 _RS11975 BPHYT_RS11980: BPHYT_RS11980 - LysR family transcriptional regulator, at 2,726,641 to 2,727,678 _RS11980 BPHYT_RS11985: BPHYT_RS11985 - hypothetical protein, at 2,727,810 to 2,728,520 _RS11985 Position (kb) 2726 2727 2728Strain fitness (log2 ratio) -1 0 1at 2725.752 kb on - strand, within BPHYT_RS11975at 2725.786 kb on + strand, within BPHYT_RS11975at 2725.791 kb on - strand, within BPHYT_RS11975at 2726.501 kb on + strandat 2727.232 kb on + strand, within BPHYT_RS11980at 2727.258 kb on + strand, within BPHYT_RS11980at 2727.536 kb on - strand, within BPHYT_RS11980at 2727.762 kb on + strandat 2728.022 kb on + strand, within BPHYT_RS11985at 2728.601 kb on + strandat 2728.602 kb on - strandat 2728.609 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,725,752 - BPHYT_RS11975 0.84 +0.5
2,725,786 + BPHYT_RS11975 0.87 +0.0
2,725,791 - BPHYT_RS11975 0.88 +1.2
2,726,501 + -1.2
2,727,232 + BPHYT_RS11980 0.57 +0.2
2,727,258 + BPHYT_RS11980 0.59 +0.8
2,727,536 - BPHYT_RS11980 0.86 +0.2
2,727,762 + +0.0
2,728,022 + BPHYT_RS11985 0.30 +1.2
2,728,601 + -0.8
2,728,602 - +0.1
2,728,609 + -1.6

Or see this region's nucleotide sequence