Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10500

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10495 and BPHYT_RS10500 are separated by 40 nucleotidesBPHYT_RS10500 and BPHYT_RS10505 are separated by 378 nucleotides BPHYT_RS10495: BPHYT_RS10495 - membrane protein, at 2,386,237 to 2,388,546 _RS10495 BPHYT_RS10500: BPHYT_RS10500 - acetolactate synthase, at 2,388,587 to 2,390,389 _RS10500 BPHYT_RS10505: BPHYT_RS10505 - 2-oxoglutarate dehydrogenase E1, at 2,390,768 to 2,393,617 _RS10505 Position (kb) 2388 2389 2390 2391Strain fitness (log2 ratio) -1 0 1at 2387.607 kb on - strand, within BPHYT_RS10495at 2387.728 kb on + strand, within BPHYT_RS10495at 2387.752 kb on + strand, within BPHYT_RS10495at 2387.759 kb on - strand, within BPHYT_RS10495at 2388.005 kb on - strand, within BPHYT_RS10495at 2388.058 kb on + strand, within BPHYT_RS10495at 2388.086 kb on - strand, within BPHYT_RS10495at 2388.088 kb on + strand, within BPHYT_RS10495at 2388.182 kb on - strand, within BPHYT_RS10495at 2388.214 kb on + strand, within BPHYT_RS10495at 2388.422 kb on - strandat 2388.509 kb on - strandat 2389.265 kb on - strand, within BPHYT_RS10500at 2390.536 kb on + strandat 2391.094 kb on - strand, within BPHYT_RS10505

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,387,607 - BPHYT_RS10495 0.59 -0.4
2,387,728 + BPHYT_RS10495 0.65 +0.6
2,387,752 + BPHYT_RS10495 0.66 +0.3
2,387,759 - BPHYT_RS10495 0.66 -0.7
2,388,005 - BPHYT_RS10495 0.77 +0.2
2,388,058 + BPHYT_RS10495 0.79 +1.1
2,388,086 - BPHYT_RS10495 0.80 +0.2
2,388,088 + BPHYT_RS10495 0.80 -0.1
2,388,182 - BPHYT_RS10495 0.84 +0.3
2,388,214 + BPHYT_RS10495 0.86 -0.6
2,388,422 - +0.3
2,388,509 - +0.8
2,389,265 - BPHYT_RS10500 0.38 +1.1
2,390,536 + -0.4
2,391,094 - BPHYT_RS10505 0.11 -0.1

Or see this region's nucleotide sequence