Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10475

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10470 and BPHYT_RS10475 are separated by 163 nucleotidesBPHYT_RS10475 and BPHYT_RS10480 are separated by 6 nucleotides BPHYT_RS10470: BPHYT_RS10470 - hypothetical protein, at 2,380,124 to 2,381,086 _RS10470 BPHYT_RS10475: BPHYT_RS10475 - mammalian cell entry protein, at 2,381,250 to 2,382,869 _RS10475 BPHYT_RS10480: BPHYT_RS10480 - LPS biosynthesis protein, at 2,382,876 to 2,385,161 _RS10480 Position (kb) 2381 2382 2383Strain fitness (log2 ratio) -1 0 1 2at 2380.664 kb on + strandat 2380.685 kb on + strand, within BPHYT_RS10470at 2380.823 kb on + strand, within BPHYT_RS10470at 2380.823 kb on + strand, within BPHYT_RS10470at 2380.824 kb on - strand, within BPHYT_RS10470at 2381.255 kb on + strandat 2381.470 kb on - strand, within BPHYT_RS10475at 2383.061 kb on - strandat 2383.253 kb on + strand, within BPHYT_RS10480at 2383.710 kb on + strandat 2383.710 kb on + strand, within BPHYT_RS10480at 2383.710 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,380,664 + -0.4
2,380,685 + BPHYT_RS10470 0.58 -0.5
2,380,823 + BPHYT_RS10470 0.73 +0.9
2,380,823 + BPHYT_RS10470 0.73 +1.0
2,380,824 - BPHYT_RS10470 0.73 +2.2
2,381,255 + +1.0
2,381,470 - BPHYT_RS10475 0.14 +1.1
2,383,061 - +1.2
2,383,253 + BPHYT_RS10480 0.16 +0.1
2,383,710 + +2.0
2,383,710 + BPHYT_RS10480 0.36 -1.1
2,383,710 + +2.2

Or see this region's nucleotide sequence