Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10350

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10340 and BPHYT_RS10345 are separated by 37 nucleotidesBPHYT_RS10345 and BPHYT_RS10350 are separated by 23 nucleotidesBPHYT_RS10350 and BPHYT_RS10355 are separated by 322 nucleotides BPHYT_RS10340: BPHYT_RS10340 - sensor protein Chase2, at 2,349,958 to 2,351,352 _RS10340 BPHYT_RS10345: BPHYT_RS10345 - flagellar motor protein MotB, at 2,351,390 to 2,351,977 _RS10345 BPHYT_RS10350: BPHYT_RS10350 - hypothetical protein, at 2,352,001 to 2,352,468 _RS10350 BPHYT_RS10355: BPHYT_RS10355 - nitrate reductase, at 2,352,791 to 2,354,248 _RS10355 Position (kb) 2352 2353Strain fitness (log2 ratio) -1 0 1at 2351.829 kb on + strand, within BPHYT_RS10345at 2352.183 kb on + strand, within BPHYT_RS10350at 2352.183 kb on + strand, within BPHYT_RS10350at 2352.303 kb on - strand, within BPHYT_RS10350at 2352.560 kb on - strandat 2352.654 kb on - strandat 2352.821 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,351,829 + BPHYT_RS10345 0.75 -0.1
2,352,183 + BPHYT_RS10350 0.39 +1.1
2,352,183 + BPHYT_RS10350 0.39 +1.1
2,352,303 - BPHYT_RS10350 0.65 +0.7
2,352,560 - +0.0
2,352,654 - +1.0
2,352,821 + +1.2

Or see this region's nucleotide sequence