Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10150

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10145 and BPHYT_RS10150 are separated by 563 nucleotidesBPHYT_RS10150 and BPHYT_RS10155 are separated by 104 nucleotides BPHYT_RS10145: BPHYT_RS10145 - Mn-containing catalase, at 2,308,872 to 2,309,744 _RS10145 BPHYT_RS10150: BPHYT_RS10150 - LysR family transcriptional regulator, at 2,310,308 to 2,311,165 _RS10150 BPHYT_RS10155: BPHYT_RS10155 - 2,2-dialkylglycine decarboxylase, at 2,311,270 to 2,312,571 _RS10155 Position (kb) 2310 2311 2312Strain fitness (log2 ratio) -1 0 1 2 3at 2309.403 kb on + strand, within BPHYT_RS10145at 2309.404 kb on - strand, within BPHYT_RS10145at 2309.802 kb on + strandat 2309.803 kb on - strandat 2309.803 kb on - strandat 2310.104 kb on + strandat 2310.105 kb on - strandat 2310.116 kb on - strandat 2310.218 kb on + strandat 2310.392 kb on - strandat 2310.394 kb on + strand, within BPHYT_RS10150at 2310.398 kb on + strand, within BPHYT_RS10150at 2310.398 kb on + strand, within BPHYT_RS10150at 2310.399 kb on - strand, within BPHYT_RS10150at 2311.654 kb on + strand, within BPHYT_RS10155at 2311.717 kb on + strand, within BPHYT_RS10155at 2311.717 kb on + strand, within BPHYT_RS10155at 2311.787 kb on - strand, within BPHYT_RS10155at 2311.942 kb on + strand, within BPHYT_RS10155at 2312.162 kb on - strand, within BPHYT_RS10155

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,309,403 + BPHYT_RS10145 0.61 -0.8
2,309,404 - BPHYT_RS10145 0.61 -0.3
2,309,802 + +0.3
2,309,803 - +0.2
2,309,803 - +0.1
2,310,104 + -0.4
2,310,105 - +0.1
2,310,116 - +1.3
2,310,218 + -0.5
2,310,392 - +0.9
2,310,394 + BPHYT_RS10150 0.10 -0.1
2,310,398 + BPHYT_RS10150 0.10 +3.0
2,310,398 + BPHYT_RS10150 0.10 +1.3
2,310,399 - BPHYT_RS10150 0.11 +0.0
2,311,654 + BPHYT_RS10155 0.29 -0.6
2,311,717 + BPHYT_RS10155 0.34 +1.8
2,311,717 + BPHYT_RS10155 0.34 -0.8
2,311,787 - BPHYT_RS10155 0.40 +1.3
2,311,942 + BPHYT_RS10155 0.52 -1.4
2,312,162 - BPHYT_RS10155 0.69 -0.3

Or see this region's nucleotide sequence