Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05835 and BPHYT_RS05840 overlap by 13 nucleotides BPHYT_RS05840 and BPHYT_RS05845 overlap by 4 nucleotides BPHYT_RS05845 and BPHYT_RS05850 are separated by 13 nucleotides BPHYT_RS05850 and BPHYT_RS05855 are separated by 511 nucleotides
BPHYT_RS05835: BPHYT_RS05835 - hypothetical protein, at 1,328,441 to 1,328,845
_RS05835
BPHYT_RS05840: BPHYT_RS05840 - hypothetical protein, at 1,328,833 to 1,329,240
_RS05840
BPHYT_RS05845: BPHYT_RS05845 - hypothetical protein, at 1,329,237 to 1,329,659
_RS05845
BPHYT_RS05850: BPHYT_RS05850 - hypothetical protein, at 1,329,673 to 1,329,930
_RS05850
BPHYT_RS05855: BPHYT_RS05855 - restriction endonuclease subunit M, at 1,330,442 to 1,331,176
_RS05855
Position (kb) 1329
1330 Strain fitness (log2 ratio) -1
0
1
2 at 1328.685 kb on - strand, within BPHYT_RS05835 at 1328.705 kb on + strand, within BPHYT_RS05835 at 1328.876 kb on + strand, within BPHYT_RS05840 at 1328.877 kb on - strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1329.558 kb on + strand, within BPHYT_RS05845 at 1329.559 kb on - strand, within BPHYT_RS05845 at 1329.669 kb on - strand at 1329.734 kb on + strand, within BPHYT_RS05850 at 1329.849 kb on - strand, within BPHYT_RS05850 at 1330.031 kb on + strand at 1330.190 kb on - strand at 1330.203 kb on - strand at 1330.227 kb on + strand at 1330.228 kb on - strand at 1330.391 kb on + strand at 1330.391 kb on + strand at 1330.391 kb on + strand at 1330.441 kb on + strand at 1330.761 kb on - strand, within BPHYT_RS05855 at 1330.761 kb on - strand, within BPHYT_RS05855 at 1330.895 kb on - strand, within BPHYT_RS05855
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,328,685 - BPHYT_RS05835 0.60 +0.1 1,328,705 + BPHYT_RS05835 0.65 -0.4 1,328,876 + BPHYT_RS05840 0.11 +0.2 1,328,877 - BPHYT_RS05840 0.11 -0.4 1,328,893 + BPHYT_RS05840 0.15 -0.0 1,328,893 + BPHYT_RS05840 0.15 -0.6 1,328,893 + BPHYT_RS05840 0.15 +0.4 1,329,558 + BPHYT_RS05845 0.76 +0.0 1,329,559 - BPHYT_RS05845 0.76 -0.7 1,329,669 - +0.2 1,329,734 + BPHYT_RS05850 0.24 +2.1 1,329,849 - BPHYT_RS05850 0.68 +0.5 1,330,031 + -0.7 1,330,190 - -0.5 1,330,203 - +0.1 1,330,227 + -0.5 1,330,228 - -0.2 1,330,391 + +0.0 1,330,391 + -0.7 1,330,391 + +1.6 1,330,441 + +0.7 1,330,761 - BPHYT_RS05855 0.43 -0.7 1,330,761 - BPHYT_RS05855 0.43 +0.2 1,330,895 - BPHYT_RS05855 0.62 +0.7
Or see this region's nucleotide sequence