Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04180 and BPHYT_RS04185 are separated by 73 nucleotides BPHYT_RS04185 and BPHYT_RS04190 are separated by 95 nucleotides
BPHYT_RS04180: BPHYT_RS04180 - sugar ABC transporter substrate-binding protein, at 958,551 to 959,798
_RS04180
BPHYT_RS04185: BPHYT_RS04185 - diguanylate cyclase, at 959,872 to 961,116
_RS04185
BPHYT_RS04190: BPHYT_RS04190 - AraC family transcriptional regulator, at 961,212 to 962,168
_RS04190
Position (kb) 959
960
961
962 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 958.916 kb on - strand, within BPHYT_RS04180 at 958.916 kb on - strand, within BPHYT_RS04180 at 958.934 kb on + strand, within BPHYT_RS04180 at 958.956 kb on - strand, within BPHYT_RS04180 at 958.956 kb on - strand, within BPHYT_RS04180 at 959.407 kb on + strand, within BPHYT_RS04180 at 959.408 kb on - strand, within BPHYT_RS04180 at 959.518 kb on - strand, within BPHYT_RS04180 at 959.518 kb on - strand, within BPHYT_RS04180 at 959.646 kb on - strand, within BPHYT_RS04180 at 959.709 kb on + strand at 959.873 kb on + strand at 959.874 kb on - strand at 959.899 kb on - strand at 960.018 kb on - strand, within BPHYT_RS04185 at 960.018 kb on - strand, within BPHYT_RS04185 at 960.018 kb on - strand, within BPHYT_RS04185 at 960.018 kb on - strand, within BPHYT_RS04185 at 960.054 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.057 kb on - strand, within BPHYT_RS04185 at 960.057 kb on - strand, within BPHYT_RS04185 at 960.276 kb on + strand, within BPHYT_RS04185 at 960.277 kb on - strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.279 kb on - strand, within BPHYT_RS04185 at 960.380 kb on - strand, within BPHYT_RS04185 at 960.438 kb on - strand, within BPHYT_RS04185 at 960.470 kb on + strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.711 kb on - strand, within BPHYT_RS04185 at 960.756 kb on - strand, within BPHYT_RS04185 at 961.040 kb on + strand at 961.040 kb on + strand at 961.041 kb on - strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.225 kb on - strand at 961.225 kb on - strand at 961.267 kb on - strand at 961.356 kb on + strand, within BPHYT_RS04190 at 961.380 kb on + strand, within BPHYT_RS04190 at 961.381 kb on - strand, within BPHYT_RS04190 at 961.385 kb on - strand, within BPHYT_RS04190 at 961.672 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.767 kb on - strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.907 kb on + strand, within BPHYT_RS04190
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 958,916 - BPHYT_RS04180 0.29 -1.1 958,916 - BPHYT_RS04180 0.29 -0.3 958,934 + BPHYT_RS04180 0.31 -1.8 958,956 - BPHYT_RS04180 0.32 -0.1 958,956 - BPHYT_RS04180 0.32 +0.7 959,407 + BPHYT_RS04180 0.69 -1.1 959,408 - BPHYT_RS04180 0.69 -0.3 959,518 - BPHYT_RS04180 0.77 -1.3 959,518 - BPHYT_RS04180 0.77 +3.2 959,646 - BPHYT_RS04180 0.88 -0.3 959,709 + +0.9 959,873 + -0.9 959,874 - -1.1 959,899 - +0.1 960,018 - BPHYT_RS04185 0.12 -0.7 960,018 - BPHYT_RS04185 0.12 -0.7 960,018 - BPHYT_RS04185 0.12 +1.2 960,018 - BPHYT_RS04185 0.12 +0.8 960,054 + BPHYT_RS04185 0.15 -0.0 960,056 + BPHYT_RS04185 0.15 -0.1 960,056 + BPHYT_RS04185 0.15 -0.2 960,056 + BPHYT_RS04185 0.15 -0.9 960,056 + BPHYT_RS04185 0.15 -0.6 960,056 + BPHYT_RS04185 0.15 -0.3 960,056 + BPHYT_RS04185 0.15 -2.9 960,057 - BPHYT_RS04185 0.15 +1.6 960,057 - BPHYT_RS04185 0.15 +0.5 960,276 + BPHYT_RS04185 0.32 -0.2 960,277 - BPHYT_RS04185 0.33 +0.2 960,278 + BPHYT_RS04185 0.33 +0.7 960,278 + BPHYT_RS04185 0.33 -0.6 960,278 + BPHYT_RS04185 0.33 +1.6 960,278 + BPHYT_RS04185 0.33 +0.9 960,278 + BPHYT_RS04185 0.33 +0.7 960,279 - BPHYT_RS04185 0.33 -0.0 960,380 - BPHYT_RS04185 0.41 -0.6 960,438 - BPHYT_RS04185 0.45 +1.9 960,470 + BPHYT_RS04185 0.48 -0.5 960,703 - BPHYT_RS04185 0.67 -0.1 960,703 - BPHYT_RS04185 0.67 -0.2 960,703 - BPHYT_RS04185 0.67 -0.1 960,703 - BPHYT_RS04185 0.67 +1.6 960,710 + BPHYT_RS04185 0.67 +0.1 960,710 + BPHYT_RS04185 0.67 +0.6 960,710 + BPHYT_RS04185 0.67 -0.9 960,711 - BPHYT_RS04185 0.67 -0.3 960,756 - BPHYT_RS04185 0.71 +0.4 961,040 + +0.2 961,040 + -0.5 961,041 - -0.4 961,224 + +0.1 961,224 + +0.6 961,224 + -0.1 961,225 - +1.8 961,225 - -0.1 961,267 - +0.0 961,356 + BPHYT_RS04190 0.15 -0.6 961,380 + BPHYT_RS04190 0.18 +0.4 961,381 - BPHYT_RS04190 0.18 +0.5 961,385 - BPHYT_RS04190 0.18 +0.8 961,672 + BPHYT_RS04190 0.48 -0.1 961,766 + BPHYT_RS04190 0.58 +1.0 961,766 + BPHYT_RS04190 0.58 -1.0 961,766 + BPHYT_RS04190 0.58 -0.1 961,767 - BPHYT_RS04190 0.58 +0.6 961,905 + BPHYT_RS04190 0.72 +0.2 961,905 + BPHYT_RS04190 0.72 +0.4 961,906 - BPHYT_RS04190 0.73 +0.0 961,906 - BPHYT_RS04190 0.73 +0.1 961,907 + BPHYT_RS04190 0.73 -0.6
Or see this region's nucleotide sequence