Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02080

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02075 and BPHYT_RS02080 are separated by 205 nucleotidesBPHYT_RS02080 and BPHYT_RS02085 are separated by 82 nucleotides BPHYT_RS02075: BPHYT_RS02075 - porin, at 475,485 to 476,570 _RS02075 BPHYT_RS02080: BPHYT_RS02080 - LysR family transcriptional regulator, at 476,776 to 477,714 _RS02080 BPHYT_RS02085: BPHYT_RS02085 - methylmalonyl-CoA mutase, at 477,797 to 481,693 _RS02085 Position (kb) 476 477 478Strain fitness (log2 ratio) -2 -1 0 1 2at 475.815 kb on + strand, within BPHYT_RS02075at 475.944 kb on + strand, within BPHYT_RS02075at 475.944 kb on + strand, within BPHYT_RS02075at 475.944 kb on + strand, within BPHYT_RS02075at 475.944 kb on + strand, within BPHYT_RS02075at 475.944 kb on + strand, within BPHYT_RS02075at 475.944 kb on + strandat 475.971 kb on + strand, within BPHYT_RS02075at 475.971 kb on + strand, within BPHYT_RS02075at 476.068 kb on - strand, within BPHYT_RS02075at 476.068 kb on - strand, within BPHYT_RS02075at 476.068 kb on - strand, within BPHYT_RS02075at 476.068 kb on - strand, within BPHYT_RS02075at 476.125 kb on - strand, within BPHYT_RS02075at 476.125 kb on - strand, within BPHYT_RS02075at 476.157 kb on + strand, within BPHYT_RS02075at 476.158 kb on - strand, within BPHYT_RS02075at 476.158 kb on - strand, within BPHYT_RS02075at 476.158 kb on - strand, within BPHYT_RS02075at 476.163 kb on + strand, within BPHYT_RS02075at 476.164 kb on - strand, within BPHYT_RS02075at 476.164 kb on - strand, within BPHYT_RS02075at 476.263 kb on - strand, within BPHYT_RS02075at 476.295 kb on + strand, within BPHYT_RS02075at 476.295 kb on + strand, within BPHYT_RS02075at 476.301 kb on + strand, within BPHYT_RS02075at 476.301 kb on + strand, within BPHYT_RS02075at 476.301 kb on + strand, within BPHYT_RS02075at 476.301 kb on + strand, within BPHYT_RS02075at 476.301 kb on + strand, within BPHYT_RS02075at 476.301 kb on + strandat 476.302 kb on - strand, within BPHYT_RS02075at 476.302 kb on - strand, within BPHYT_RS02075at 476.307 kb on + strand, within BPHYT_RS02075at 476.308 kb on - strand, within BPHYT_RS02075at 476.308 kb on - strand, within BPHYT_RS02075at 476.308 kb on - strand, within BPHYT_RS02075at 476.308 kb on - strand, within BPHYT_RS02075at 476.458 kb on - strand, within BPHYT_RS02075at 476.491 kb on - strandat 476.491 kb on - strandat 476.499 kb on + strandat 476.499 kb on + strandat 476.500 kb on - strandat 476.500 kb on - strandat 476.505 kb on + strandat 476.505 kb on + strandat 476.505 kb on + strandat 476.505 kb on + strandat 476.505 kb on + strandat 476.505 kb on + strandat 476.505 kb on + strandat 476.506 kb on - strandat 476.506 kb on - strandat 476.506 kb on - strandat 476.506 kb on - strandat 476.506 kb on - strandat 476.541 kb on + strandat 476.615 kb on + strandat 476.729 kb on - strandat 476.782 kb on - strandat 476.972 kb on - strand, within BPHYT_RS02080at 477.149 kb on + strand, within BPHYT_RS02080at 477.149 kb on + strand, within BPHYT_RS02080at 477.150 kb on - strand, within BPHYT_RS02080at 477.346 kb on + strand, within BPHYT_RS02080at 477.346 kb on + strand, within BPHYT_RS02080at 477.347 kb on - strand, within BPHYT_RS02080at 477.347 kb on - strand, within BPHYT_RS02080at 477.592 kb on + strand, within BPHYT_RS02080at 477.593 kb on - strand, within BPHYT_RS02080at 477.753 kb on - strandat 477.754 kb on + strandat 477.755 kb on - strandat 477.773 kb on + strandat 477.773 kb on + strandat 477.774 kb on - strandat 478.034 kb on + strandat 478.035 kb on - strandat 478.065 kb on - strandat 478.184 kb on + strandat 478.184 kb on + strandat 478.184 kb on + strandat 478.185 kb on - strandat 478.185 kb on - strandat 478.235 kb on - strand, within BPHYT_RS02085

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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475,815 + BPHYT_RS02075 0.30 -0.3
475,944 + BPHYT_RS02075 0.42 +0.1
475,944 + BPHYT_RS02075 0.42 +0.3
475,944 + BPHYT_RS02075 0.42 -0.1
475,944 + BPHYT_RS02075 0.42 +1.2
475,944 + BPHYT_RS02075 0.42 +0.0
475,944 + -1.0
475,971 + BPHYT_RS02075 0.45 -0.0
475,971 + BPHYT_RS02075 0.45 +0.6
476,068 - BPHYT_RS02075 0.54 +1.0
476,068 - BPHYT_RS02075 0.54 -2.0
476,068 - BPHYT_RS02075 0.54 -0.6
476,068 - BPHYT_RS02075 0.54 +0.3
476,125 - BPHYT_RS02075 0.59 +0.2
476,125 - BPHYT_RS02075 0.59 +0.0
476,157 + BPHYT_RS02075 0.62 -0.2
476,158 - BPHYT_RS02075 0.62 -1.5
476,158 - BPHYT_RS02075 0.62 +0.6
476,158 - BPHYT_RS02075 0.62 +1.1
476,163 + BPHYT_RS02075 0.62 +0.6
476,164 - BPHYT_RS02075 0.63 -2.3
476,164 - BPHYT_RS02075 0.63 -0.6
476,263 - BPHYT_RS02075 0.72 +2.2
476,295 + BPHYT_RS02075 0.75 +0.1
476,295 + BPHYT_RS02075 0.75 -0.1
476,301 + BPHYT_RS02075 0.75 -0.1
476,301 + BPHYT_RS02075 0.75 +1.2
476,301 + BPHYT_RS02075 0.75 +0.6
476,301 + BPHYT_RS02075 0.75 +0.3
476,301 + BPHYT_RS02075 0.75 +0.5
476,301 + -1.5
476,302 - BPHYT_RS02075 0.75 +0.6
476,302 - BPHYT_RS02075 0.75 +0.3
476,307 + BPHYT_RS02075 0.76 +0.4
476,308 - BPHYT_RS02075 0.76 +0.8
476,308 - BPHYT_RS02075 0.76 -0.2
476,308 - BPHYT_RS02075 0.76 -0.1
476,308 - BPHYT_RS02075 0.76 +1.8
476,458 - BPHYT_RS02075 0.90 -0.2
476,491 - -0.3
476,491 - +0.2
476,499 + +0.9
476,499 + +0.3
476,500 - -1.4
476,500 - -1.1
476,505 + -0.2
476,505 + -1.7
476,505 + +0.6
476,505 + +1.4
476,505 + -0.8
476,505 + -0.9
476,505 + +2.2
476,506 - +0.2
476,506 - -2.1
476,506 - +0.8
476,506 - -0.2
476,506 - +0.1
476,541 + -0.7
476,615 + -1.0
476,729 - -0.3
476,782 - +0.4
476,972 - BPHYT_RS02080 0.21 +0.9
477,149 + BPHYT_RS02080 0.40 -2.4
477,149 + BPHYT_RS02080 0.40 +0.3
477,150 - BPHYT_RS02080 0.40 +0.2
477,346 + BPHYT_RS02080 0.61 -0.4
477,346 + BPHYT_RS02080 0.61 +0.3
477,347 - BPHYT_RS02080 0.61 +0.6
477,347 - BPHYT_RS02080 0.61 +2.6
477,592 + BPHYT_RS02080 0.87 -1.5
477,593 - BPHYT_RS02080 0.87 -0.2
477,753 - +1.6
477,754 + +1.2
477,755 - -0.5
477,773 + +1.3
477,773 + +0.7
477,774 - -0.4
478,034 + -0.3
478,035 - +1.1
478,065 - -1.2
478,184 + -0.1
478,184 + +1.4
478,184 + -0.7
478,185 - +0.7
478,185 - -2.3
478,235 - BPHYT_RS02085 0.11 -0.1

Or see this region's nucleotide sequence