Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01775 and BPHYT_RS01780 overlap by 4 nucleotides BPHYT_RS01780 and BPHYT_RS01785 are separated by 375 nucleotides BPHYT_RS01785 and BPHYT_RS01790 are separated by 294 nucleotides BPHYT_RS01790 and BPHYT_RS01795 are separated by 10 nucleotides
BPHYT_RS01775: BPHYT_RS01775 - hypothetical protein, at 404,469 to 404,813
_RS01775
BPHYT_RS01780: BPHYT_RS01780 - hypothetical protein, at 404,810 to 405,178
_RS01780
BPHYT_RS01785: BPHYT_RS01785 - amino acid permease, at 405,554 to 406,945
_RS01785
BPHYT_RS01790: BPHYT_RS01790 - LuxR family transcriptional regulator, at 407,240 to 407,896
_RS01790
BPHYT_RS01795: BPHYT_RS01795 - histidine kinase, at 407,907 to 408,785
_RS01795
Position (kb) 405
406
407 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 404.588 kb on + strand, within BPHYT_RS01775 at 404.589 kb on - strand, within BPHYT_RS01775 at 404.596 kb on + strand, within BPHYT_RS01775 at 404.597 kb on - strand, within BPHYT_RS01775 at 404.705 kb on - strand, within BPHYT_RS01775 at 404.717 kb on + strand, within BPHYT_RS01775 at 404.718 kb on - strand, within BPHYT_RS01775 at 404.720 kb on + strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.820 kb on - strand at 404.867 kb on + strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.876 kb on - strand, within BPHYT_RS01780 at 405.024 kb on - strand, within BPHYT_RS01780 at 405.144 kb on - strand at 405.146 kb on - strand at 405.214 kb on + strand at 405.214 kb on + strand at 405.284 kb on - strand at 405.325 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.527 kb on + strand at 405.797 kb on - strand, within BPHYT_RS01785 at 405.880 kb on + strand at 405.986 kb on + strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 406.069 kb on - strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.144 kb on - strand, within BPHYT_RS01785 at 406.144 kb on - strand, within BPHYT_RS01785 at 406.564 kb on - strand at 406.662 kb on + strand, within BPHYT_RS01785 at 406.662 kb on + strand, within BPHYT_RS01785 at 406.662 kb on + strand, within BPHYT_RS01785 at 406.668 kb on + strand, within BPHYT_RS01785 at 406.668 kb on + strand, within BPHYT_RS01785 at 406.668 kb on + strand, within BPHYT_RS01785 at 406.668 kb on + strand, within BPHYT_RS01785 at 406.668 kb on + strand, within BPHYT_RS01785 at 406.668 kb on + strand, within BPHYT_RS01785 at 406.669 kb on - strand, within BPHYT_RS01785 at 406.669 kb on - strand, within BPHYT_RS01785 at 406.669 kb on - strand, within BPHYT_RS01785 at 406.669 kb on - strand, within BPHYT_RS01785 at 406.674 kb on + strand, within BPHYT_RS01785 at 406.675 kb on - strand, within BPHYT_RS01785 at 406.675 kb on - strand, within BPHYT_RS01785 at 406.675 kb on - strand, within BPHYT_RS01785 at 406.675 kb on - strand, within BPHYT_RS01785 at 406.688 kb on + strand, within BPHYT_RS01785 at 406.716 kb on + strand, within BPHYT_RS01785 at 406.831 kb on + strand at 406.961 kb on + strand at 406.961 kb on + strand at 407.007 kb on + strand at 407.289 kb on + strand at 407.289 kb on + strand at 407.289 kb on + strand at 407.290 kb on - strand at 407.290 kb on - strand at 407.290 kb on - strand at 407.389 kb on + strand, within BPHYT_RS01790 at 407.434 kb on - strand, within BPHYT_RS01790 at 407.462 kb on + strand, within BPHYT_RS01790 at 407.462 kb on + strand, within BPHYT_RS01790 at 407.487 kb on + strand, within BPHYT_RS01790 at 407.523 kb on + strand, within BPHYT_RS01790 at 407.523 kb on + strand, within BPHYT_RS01790 at 407.524 kb on - strand, within BPHYT_RS01790 at 407.524 kb on - strand, within BPHYT_RS01790 at 407.629 kb on - strand, within BPHYT_RS01790 at 407.629 kb on - strand, within BPHYT_RS01790 at 407.629 kb on - strand, within BPHYT_RS01790 at 407.644 kb on - strand, within BPHYT_RS01790 at 407.644 kb on - strand, within BPHYT_RS01790 at 407.752 kb on - strand, within BPHYT_RS01790 at 407.752 kb on - strand, within BPHYT_RS01790 at 407.859 kb on - strand at 407.904 kb on + strand at 407.904 kb on + strand at 407.905 kb on - strand at 407.905 kb on - strand at 407.905 kb on - strand at 407.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 404,588 + BPHYT_RS01775 0.34 -0.3 404,589 - BPHYT_RS01775 0.35 +0.4 404,596 + BPHYT_RS01775 0.37 -0.8 404,597 - BPHYT_RS01775 0.37 -0.4 404,705 - BPHYT_RS01775 0.68 -0.5 404,717 + BPHYT_RS01775 0.72 +0.6 404,718 - BPHYT_RS01775 0.72 -0.3 404,720 + BPHYT_RS01775 0.73 -0.8 404,721 - BPHYT_RS01775 0.73 -0.3 404,721 - BPHYT_RS01775 0.73 -0.1 404,820 - +0.3 404,867 + BPHYT_RS01780 0.15 +0.7 404,868 - BPHYT_RS01780 0.16 +0.3 404,868 - BPHYT_RS01780 0.16 -0.3 404,868 - BPHYT_RS01780 0.16 -0.3 404,876 - BPHYT_RS01780 0.18 +0.5 405,024 - BPHYT_RS01780 0.58 -0.3 405,144 - -0.2 405,146 - +0.6 405,214 + -0.5 405,214 + -0.3 405,284 - -1.6 405,325 - +0.8 405,373 + +0.1 405,373 + +0.5 405,527 + +0.3 405,797 - BPHYT_RS01785 0.17 +0.9 405,880 + +0.3 405,986 + BPHYT_RS01785 0.31 +0.7 405,987 - BPHYT_RS01785 0.31 +0.0 405,987 - BPHYT_RS01785 0.31 -0.0 405,987 - BPHYT_RS01785 0.31 -0.3 406,069 - BPHYT_RS01785 0.37 +1.2 406,143 + BPHYT_RS01785 0.42 +0.1 406,143 + BPHYT_RS01785 0.42 +0.5 406,143 + BPHYT_RS01785 0.42 -0.8 406,143 + BPHYT_RS01785 0.42 -0.9 406,143 + BPHYT_RS01785 0.42 -0.2 406,144 - BPHYT_RS01785 0.42 -1.7 406,144 - BPHYT_RS01785 0.42 -0.2 406,564 - -0.3 406,662 + BPHYT_RS01785 0.80 +1.6 406,662 + BPHYT_RS01785 0.80 -0.4 406,662 + BPHYT_RS01785 0.80 -0.3 406,668 + BPHYT_RS01785 0.80 +0.0 406,668 + BPHYT_RS01785 0.80 -1.1 406,668 + BPHYT_RS01785 0.80 +1.3 406,668 + BPHYT_RS01785 0.80 -0.7 406,668 + BPHYT_RS01785 0.80 -0.2 406,668 + BPHYT_RS01785 0.80 -0.5 406,669 - BPHYT_RS01785 0.80 -0.1 406,669 - BPHYT_RS01785 0.80 -0.2 406,669 - BPHYT_RS01785 0.80 -0.9 406,669 - BPHYT_RS01785 0.80 +0.1 406,674 + BPHYT_RS01785 0.80 -0.9 406,675 - BPHYT_RS01785 0.81 +0.1 406,675 - BPHYT_RS01785 0.81 -1.6 406,675 - BPHYT_RS01785 0.81 +0.1 406,675 - BPHYT_RS01785 0.81 -0.9 406,688 + BPHYT_RS01785 0.81 +0.4 406,716 + BPHYT_RS01785 0.83 -0.3 406,831 + -0.7 406,961 + +0.4 406,961 + +0.3 407,007 + -0.5 407,289 + -6.0 407,289 + -4.3 407,289 + -1.9 407,290 - -3.6 407,290 - -3.6 407,290 - -5.0 407,389 + BPHYT_RS01790 0.23 -2.2 407,434 - BPHYT_RS01790 0.30 -4.2 407,462 + BPHYT_RS01790 0.34 -2.6 407,462 + BPHYT_RS01790 0.34 -4.7 407,487 + BPHYT_RS01790 0.38 -5.5 407,523 + BPHYT_RS01790 0.43 -6.0 407,523 + BPHYT_RS01790 0.43 -3.8 407,524 - BPHYT_RS01790 0.43 -2.2 407,524 - BPHYT_RS01790 0.43 -3.9 407,629 - BPHYT_RS01790 0.59 -3.3 407,629 - BPHYT_RS01790 0.59 -2.1 407,629 - BPHYT_RS01790 0.59 -5.0 407,644 - BPHYT_RS01790 0.61 -7.0 407,644 - BPHYT_RS01790 0.61 -4.9 407,752 - BPHYT_RS01790 0.78 -4.8 407,752 - BPHYT_RS01790 0.78 -4.2 407,859 - -4.4 407,904 + -4.3 407,904 + -4.8 407,905 - -0.7 407,905 - -3.5 407,905 - -1.4 407,905 - -0.6
Or see this region's nucleotide sequence