Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32485

Experiment: L-Valine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32475 and RR42_RS32480 are separated by 24 nucleotidesRR42_RS32480 and RR42_RS32485 are separated by 131 nucleotidesRR42_RS32485 and RR42_RS32490 are separated by 46 nucleotidesRR42_RS32490 and RR42_RS32495 are separated by 123 nucleotides RR42_RS32475: RR42_RS32475 - permease, at 2,683,242 to 2,684,201 _RS32475 RR42_RS32480: RR42_RS32480 - 4-hydroxyphenylpyruvate dioxygenase, at 2,684,226 to 2,684,993 _RS32480 RR42_RS32485: RR42_RS32485 - 3-keto-5-aminohexanoate cleavage protein, at 2,685,125 to 2,687,008 _RS32485 RR42_RS32490: RR42_RS32490 - 3-keto-5-aminohexanoate cleavage protein, at 2,687,055 to 2,687,879 _RS32490 RR42_RS32495: RR42_RS32495 - shikimate kinase, at 2,688,003 to 2,688,515 _RS32495 Position (kb) 2685 2686 2687 2688Strain fitness (log2 ratio) -2 -1 0 1 2at 2684.319 kb on - strand, within RR42_RS32480at 2684.417 kb on - strand, within RR42_RS32480at 2684.485 kb on + strand, within RR42_RS32480at 2684.485 kb on + strand, within RR42_RS32480at 2684.593 kb on - strand, within RR42_RS32480at 2684.644 kb on - strand, within RR42_RS32480at 2684.675 kb on - strand, within RR42_RS32480at 2684.752 kb on + strand, within RR42_RS32480at 2684.843 kb on - strand, within RR42_RS32480at 2685.078 kb on + strandat 2685.078 kb on + strandat 2685.079 kb on - strandat 2685.090 kb on + strandat 2685.303 kb on + strandat 2685.304 kb on - strandat 2685.304 kb on - strandat 2685.304 kb on - strandat 2685.323 kb on + strand, within RR42_RS32485at 2685.406 kb on - strand, within RR42_RS32485at 2685.542 kb on + strand, within RR42_RS32485at 2685.542 kb on + strand, within RR42_RS32485at 2685.543 kb on - strand, within RR42_RS32485at 2685.543 kb on - strand, within RR42_RS32485at 2685.543 kb on - strand, within RR42_RS32485at 2685.640 kb on + strand, within RR42_RS32485at 2685.640 kb on + strand, within RR42_RS32485at 2686.331 kb on + strand, within RR42_RS32485at 2686.368 kb on - strand, within RR42_RS32485at 2686.487 kb on + strand, within RR42_RS32485at 2686.487 kb on + strand, within RR42_RS32485at 2686.488 kb on - strand, within RR42_RS32485at 2686.542 kb on - strand, within RR42_RS32485at 2686.757 kb on + strand, within RR42_RS32485at 2686.757 kb on + strand, within RR42_RS32485at 2686.758 kb on - strand, within RR42_RS32485at 2686.758 kb on - strand, within RR42_RS32485at 2686.758 kb on - strand, within RR42_RS32485at 2686.758 kb on - strand, within RR42_RS32485at 2686.758 kb on - strand, within RR42_RS32485at 2686.760 kb on + strand, within RR42_RS32485at 2686.760 kb on + strand, within RR42_RS32485at 2686.761 kb on - strand, within RR42_RS32485at 2686.830 kb on + strandat 2686.831 kb on - strandat 2686.868 kb on + strandat 2686.868 kb on + strandat 2686.869 kb on - strandat 2686.869 kb on - strandat 2686.923 kb on - strandat 2687.460 kb on + strand, within RR42_RS32490at 2687.460 kb on + strand, within RR42_RS32490at 2687.461 kb on - strand, within RR42_RS32490at 2687.505 kb on + strand, within RR42_RS32490at 2687.506 kb on - strand, within RR42_RS32490at 2687.506 kb on - strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.638 kb on + strand, within RR42_RS32490at 2687.639 kb on - strand, within RR42_RS32490at 2687.886 kb on + strandat 2687.927 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (N)
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2,684,319 - RR42_RS32480 0.12 +1.0
2,684,417 - RR42_RS32480 0.25 +1.7
2,684,485 + RR42_RS32480 0.34 -0.1
2,684,485 + RR42_RS32480 0.34 +0.0
2,684,593 - RR42_RS32480 0.48 +0.5
2,684,644 - RR42_RS32480 0.54 -0.1
2,684,675 - RR42_RS32480 0.58 -0.2
2,684,752 + RR42_RS32480 0.68 +0.1
2,684,843 - RR42_RS32480 0.80 -1.2
2,685,078 + -1.8
2,685,078 + +1.9
2,685,079 - -1.1
2,685,090 + +0.4
2,685,303 + +0.7
2,685,304 - +0.0
2,685,304 - -0.3
2,685,304 - -1.0
2,685,323 + RR42_RS32485 0.11 -0.3
2,685,406 - RR42_RS32485 0.15 -1.1
2,685,542 + RR42_RS32485 0.22 +1.0
2,685,542 + RR42_RS32485 0.22 -0.6
2,685,543 - RR42_RS32485 0.22 +0.7
2,685,543 - RR42_RS32485 0.22 +0.6
2,685,543 - RR42_RS32485 0.22 +0.0
2,685,640 + RR42_RS32485 0.27 -0.5
2,685,640 + RR42_RS32485 0.27 -0.3
2,686,331 + RR42_RS32485 0.64 -0.1
2,686,368 - RR42_RS32485 0.66 -0.3
2,686,487 + RR42_RS32485 0.72 -1.0
2,686,487 + RR42_RS32485 0.72 +0.3
2,686,488 - RR42_RS32485 0.72 -0.0
2,686,542 - RR42_RS32485 0.75 -0.7
2,686,757 + RR42_RS32485 0.87 -0.2
2,686,757 + RR42_RS32485 0.87 +1.0
2,686,758 - RR42_RS32485 0.87 -1.2
2,686,758 - RR42_RS32485 0.87 +0.4
2,686,758 - RR42_RS32485 0.87 -0.2
2,686,758 - RR42_RS32485 0.87 -2.3
2,686,758 - RR42_RS32485 0.87 -0.1
2,686,760 + RR42_RS32485 0.87 +1.0
2,686,760 + RR42_RS32485 0.87 -1.8
2,686,761 - RR42_RS32485 0.87 -1.9
2,686,830 + +1.0
2,686,831 - +0.6
2,686,868 + +0.8
2,686,868 + -0.4
2,686,869 - +2.6
2,686,869 - -0.1
2,686,923 - +1.0
2,687,460 + RR42_RS32490 0.49 +0.8
2,687,460 + RR42_RS32490 0.49 +0.0
2,687,461 - RR42_RS32490 0.49 -0.2
2,687,505 + RR42_RS32490 0.55 +0.5
2,687,506 - RR42_RS32490 0.55 +0.5
2,687,506 - RR42_RS32490 0.55 +1.2
2,687,638 + RR42_RS32490 0.71 +0.6
2,687,638 + RR42_RS32490 0.71 -0.2
2,687,638 + RR42_RS32490 0.71 -1.1
2,687,638 + RR42_RS32490 0.71 -0.3
2,687,638 + RR42_RS32490 0.71 -1.3
2,687,638 + RR42_RS32490 0.71 +0.2
2,687,638 + RR42_RS32490 0.71 -0.5
2,687,638 + RR42_RS32490 0.71 -2.3
2,687,639 - RR42_RS32490 0.71 -0.6
2,687,886 + -0.2
2,687,927 + +0.4

Or see this region's nucleotide sequence