Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25210

Experiment: L-Valine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25205 and RR42_RS25210 are separated by 172 nucleotidesRR42_RS25210 and RR42_RS25215 are separated by 139 nucleotidesRR42_RS25215 and RR42_RS25220 are separated by 154 nucleotides RR42_RS25205: RR42_RS25205 - glucose 1-dehydrogenase, at 996,447 to 997,181 _RS25205 RR42_RS25210: RR42_RS25210 - acetyl-CoA acetyltransferase, at 997,354 to 998,538 _RS25210 RR42_RS25215: RR42_RS25215 - DSBA oxidoreductase, at 998,678 to 999,301 _RS25215 RR42_RS25220: RR42_RS25220 - NADP transhydrogenase subunit alpha, at 999,456 to 1,000,574 _RS25220 Position (kb) 997 998 999Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 996.355 kb on - strandat 996.355 kb on - strandat 996.355 kb on - strandat 996.355 kb on - strandat 996.618 kb on + strand, within RR42_RS25205at 996.619 kb on - strand, within RR42_RS25205at 996.619 kb on - strand, within RR42_RS25205at 996.709 kb on + strand, within RR42_RS25205at 996.872 kb on + strand, within RR42_RS25205at 996.900 kb on + strand, within RR42_RS25205at 996.901 kb on - strand, within RR42_RS25205at 996.901 kb on - strand, within RR42_RS25205at 996.992 kb on + strand, within RR42_RS25205at 996.992 kb on + strand, within RR42_RS25205at 996.992 kb on + strand, within RR42_RS25205at 996.992 kb on + strand, within RR42_RS25205at 996.993 kb on - strand, within RR42_RS25205at 996.993 kb on - strand, within RR42_RS25205at 996.993 kb on - strand, within RR42_RS25205at 997.026 kb on - strand, within RR42_RS25205at 997.050 kb on + strand, within RR42_RS25205at 997.050 kb on + strand, within RR42_RS25205at 997.091 kb on + strand, within RR42_RS25205at 997.105 kb on - strand, within RR42_RS25205at 997.105 kb on - strand, within RR42_RS25205at 997.111 kb on - strandat 997.179 kb on + strandat 997.205 kb on + strandat 997.206 kb on - strandat 997.322 kb on + strandat 997.323 kb on - strandat 997.408 kb on - strandat 997.519 kb on - strand, within RR42_RS25210at 997.519 kb on - strand, within RR42_RS25210at 997.688 kb on + strand, within RR42_RS25210at 997.792 kb on + strand, within RR42_RS25210at 997.793 kb on - strand, within RR42_RS25210at 997.793 kb on - strand, within RR42_RS25210at 997.793 kb on - strand, within RR42_RS25210at 997.835 kb on - strand, within RR42_RS25210at 997.860 kb on + strand, within RR42_RS25210at 997.861 kb on - strand, within RR42_RS25210at 997.861 kb on - strand, within RR42_RS25210at 997.864 kb on + strand, within RR42_RS25210at 997.864 kb on + strand, within RR42_RS25210at 997.865 kb on - strand, within RR42_RS25210at 997.888 kb on - strand, within RR42_RS25210at 997.928 kb on + strand, within RR42_RS25210at 997.928 kb on + strand, within RR42_RS25210at 997.929 kb on - strand, within RR42_RS25210at 997.929 kb on - strand, within RR42_RS25210at 997.929 kb on - strand, within RR42_RS25210at 997.931 kb on + strand, within RR42_RS25210at 997.932 kb on - strand, within RR42_RS25210at 997.932 kb on - strand, within RR42_RS25210at 997.932 kb on - strand, within RR42_RS25210at 997.998 kb on - strand, within RR42_RS25210at 998.183 kb on - strand, within RR42_RS25210at 998.408 kb on - strand, within RR42_RS25210at 998.473 kb on + strandat 998.473 kb on + strandat 998.474 kb on - strandat 998.537 kb on - strandat 998.537 kb on - strandat 998.537 kb on - strandat 998.537 kb on - strandat 998.537 kb on - strandat 998.632 kb on - strandat 998.665 kb on - strandat 998.665 kb on - strandat 998.668 kb on - strandat 998.668 kb on - strandat 998.668 kb on - strandat 998.734 kb on + strandat 998.756 kb on - strand, within RR42_RS25215at 998.756 kb on - strand, within RR42_RS25215at 998.786 kb on + strand, within RR42_RS25215at 998.787 kb on - strand, within RR42_RS25215at 998.956 kb on + strand, within RR42_RS25215at 998.956 kb on + strand, within RR42_RS25215at 998.957 kb on - strand, within RR42_RS25215at 998.957 kb on - strand, within RR42_RS25215at 999.299 kb on + strandat 999.437 kb on + strandat 999.531 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (N)
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996,355 - -1.8
996,355 - +0.3
996,355 - +0.1
996,355 - +0.9
996,618 + RR42_RS25205 0.23 +0.1
996,619 - RR42_RS25205 0.23 -0.1
996,619 - RR42_RS25205 0.23 -0.3
996,709 + RR42_RS25205 0.36 +1.0
996,872 + RR42_RS25205 0.58 -0.4
996,900 + RR42_RS25205 0.62 -0.8
996,901 - RR42_RS25205 0.62 -1.0
996,901 - RR42_RS25205 0.62 +0.2
996,992 + RR42_RS25205 0.74 +0.5
996,992 + RR42_RS25205 0.74 +0.0
996,992 + RR42_RS25205 0.74 +0.4
996,992 + RR42_RS25205 0.74 -0.1
996,993 - RR42_RS25205 0.74 -0.7
996,993 - RR42_RS25205 0.74 -0.4
996,993 - RR42_RS25205 0.74 +0.2
997,026 - RR42_RS25205 0.79 -1.0
997,050 + RR42_RS25205 0.82 -1.0
997,050 + RR42_RS25205 0.82 -0.6
997,091 + RR42_RS25205 0.88 -0.6
997,105 - RR42_RS25205 0.90 -0.3
997,105 - RR42_RS25205 0.90 +0.4
997,111 - +0.6
997,179 + +0.2
997,205 + +0.8
997,206 - -0.4
997,322 + -0.4
997,323 - +0.9
997,408 - -0.7
997,519 - RR42_RS25210 0.14 +0.2
997,519 - RR42_RS25210 0.14 -1.0
997,688 + RR42_RS25210 0.28 +0.2
997,792 + RR42_RS25210 0.37 -1.4
997,793 - RR42_RS25210 0.37 -1.6
997,793 - RR42_RS25210 0.37 +0.2
997,793 - RR42_RS25210 0.37 +0.2
997,835 - RR42_RS25210 0.41 +0.7
997,860 + RR42_RS25210 0.43 -0.4
997,861 - RR42_RS25210 0.43 +0.5
997,861 - RR42_RS25210 0.43 -0.1
997,864 + RR42_RS25210 0.43 -0.1
997,864 + RR42_RS25210 0.43 +0.6
997,865 - RR42_RS25210 0.43 +1.2
997,888 - RR42_RS25210 0.45 -2.2
997,928 + RR42_RS25210 0.48 +0.4
997,928 + RR42_RS25210 0.48 -0.2
997,929 - RR42_RS25210 0.49 +0.9
997,929 - RR42_RS25210 0.49 +0.9
997,929 - RR42_RS25210 0.49 -0.1
997,931 + RR42_RS25210 0.49 +0.1
997,932 - RR42_RS25210 0.49 +0.4
997,932 - RR42_RS25210 0.49 +0.1
997,932 - RR42_RS25210 0.49 -0.2
997,998 - RR42_RS25210 0.54 -0.1
998,183 - RR42_RS25210 0.70 +0.5
998,408 - RR42_RS25210 0.89 -0.3
998,473 + -0.2
998,473 + +0.6
998,474 - -0.9
998,537 - -0.5
998,537 - +2.2
998,537 - -3.4
998,537 - +0.9
998,537 - -1.2
998,632 - -0.4
998,665 - -1.0
998,665 - -0.1
998,668 - -1.0
998,668 - -0.5
998,668 - +0.6
998,734 + +0.1
998,756 - RR42_RS25215 0.12 -0.5
998,756 - RR42_RS25215 0.12 -0.4
998,786 + RR42_RS25215 0.17 +0.6
998,787 - RR42_RS25215 0.17 -0.0
998,956 + RR42_RS25215 0.45 -0.4
998,956 + RR42_RS25215 0.45 +0.3
998,957 - RR42_RS25215 0.45 -0.5
998,957 - RR42_RS25215 0.45 -0.8
999,299 + -1.4
999,437 + -0.8
999,531 + +1.4

Or see this region's nucleotide sequence