Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25205 and RR42_RS25210 are separated by 172 nucleotides RR42_RS25210 and RR42_RS25215 are separated by 139 nucleotides RR42_RS25215 and RR42_RS25220 are separated by 154 nucleotides
RR42_RS25205: RR42_RS25205 - glucose 1-dehydrogenase, at 996,447 to 997,181
_RS25205
RR42_RS25210: RR42_RS25210 - acetyl-CoA acetyltransferase, at 997,354 to 998,538
_RS25210
RR42_RS25215: RR42_RS25215 - DSBA oxidoreductase, at 998,678 to 999,301
_RS25215
RR42_RS25220: RR42_RS25220 - NADP transhydrogenase subunit alpha, at 999,456 to 1,000,574
_RS25220
Position (kb)
997
998
999 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.618 kb on + strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.709 kb on + strand, within RR42_RS25205 at 996.872 kb on + strand, within RR42_RS25205 at 996.900 kb on + strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 997.026 kb on - strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.091 kb on + strand, within RR42_RS25205 at 997.105 kb on - strand, within RR42_RS25205 at 997.105 kb on - strand, within RR42_RS25205 at 997.111 kb on - strand at 997.179 kb on + strand at 997.205 kb on + strand at 997.206 kb on - strand at 997.322 kb on + strand at 997.323 kb on - strand at 997.408 kb on - strand at 997.519 kb on - strand, within RR42_RS25210 at 997.519 kb on - strand, within RR42_RS25210 at 997.688 kb on + strand, within RR42_RS25210 at 997.792 kb on + strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.835 kb on - strand, within RR42_RS25210 at 997.860 kb on + strand, within RR42_RS25210 at 997.861 kb on - strand, within RR42_RS25210 at 997.861 kb on - strand, within RR42_RS25210 at 997.864 kb on + strand, within RR42_RS25210 at 997.864 kb on + strand, within RR42_RS25210 at 997.865 kb on - strand, within RR42_RS25210 at 997.888 kb on - strand, within RR42_RS25210 at 997.928 kb on + strand, within RR42_RS25210 at 997.928 kb on + strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.931 kb on + strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.998 kb on - strand, within RR42_RS25210 at 998.183 kb on - strand, within RR42_RS25210 at 998.408 kb on - strand, within RR42_RS25210 at 998.473 kb on + strand at 998.473 kb on + strand at 998.474 kb on - strand at 998.537 kb on - strand at 998.537 kb on - strand at 998.537 kb on - strand at 998.537 kb on - strand at 998.537 kb on - strand at 998.632 kb on - strand at 998.665 kb on - strand at 998.665 kb on - strand at 998.668 kb on - strand at 998.668 kb on - strand at 998.668 kb on - strand at 998.734 kb on + strand at 998.756 kb on - strand, within RR42_RS25215 at 998.756 kb on - strand, within RR42_RS25215 at 998.786 kb on + strand, within RR42_RS25215 at 998.787 kb on - strand, within RR42_RS25215 at 998.956 kb on + strand, within RR42_RS25215 at 998.956 kb on + strand, within RR42_RS25215 at 998.957 kb on - strand, within RR42_RS25215 at 998.957 kb on - strand, within RR42_RS25215 at 999.299 kb on + strand at 999.437 kb on + strand at 999.531 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 996,355 - -1.8 996,355 - +0.3 996,355 - +0.1 996,355 - +0.9 996,618 + RR42_RS25205 0.23 +0.1 996,619 - RR42_RS25205 0.23 -0.1 996,619 - RR42_RS25205 0.23 -0.3 996,709 + RR42_RS25205 0.36 +1.0 996,872 + RR42_RS25205 0.58 -0.4 996,900 + RR42_RS25205 0.62 -0.8 996,901 - RR42_RS25205 0.62 -1.0 996,901 - RR42_RS25205 0.62 +0.2 996,992 + RR42_RS25205 0.74 +0.5 996,992 + RR42_RS25205 0.74 +0.0 996,992 + RR42_RS25205 0.74 +0.4 996,992 + RR42_RS25205 0.74 -0.1 996,993 - RR42_RS25205 0.74 -0.7 996,993 - RR42_RS25205 0.74 -0.4 996,993 - RR42_RS25205 0.74 +0.2 997,026 - RR42_RS25205 0.79 -1.0 997,050 + RR42_RS25205 0.82 -1.0 997,050 + RR42_RS25205 0.82 -0.6 997,091 + RR42_RS25205 0.88 -0.6 997,105 - RR42_RS25205 0.90 -0.3 997,105 - RR42_RS25205 0.90 +0.4 997,111 - +0.6 997,179 + +0.2 997,205 + +0.8 997,206 - -0.4 997,322 + -0.4 997,323 - +0.9 997,408 - -0.7 997,519 - RR42_RS25210 0.14 +0.2 997,519 - RR42_RS25210 0.14 -1.0 997,688 + RR42_RS25210 0.28 +0.2 997,792 + RR42_RS25210 0.37 -1.4 997,793 - RR42_RS25210 0.37 -1.6 997,793 - RR42_RS25210 0.37 +0.2 997,793 - RR42_RS25210 0.37 +0.2 997,835 - RR42_RS25210 0.41 +0.7 997,860 + RR42_RS25210 0.43 -0.4 997,861 - RR42_RS25210 0.43 +0.5 997,861 - RR42_RS25210 0.43 -0.1 997,864 + RR42_RS25210 0.43 -0.1 997,864 + RR42_RS25210 0.43 +0.6 997,865 - RR42_RS25210 0.43 +1.2 997,888 - RR42_RS25210 0.45 -2.2 997,928 + RR42_RS25210 0.48 +0.4 997,928 + RR42_RS25210 0.48 -0.2 997,929 - RR42_RS25210 0.49 +0.9 997,929 - RR42_RS25210 0.49 +0.9 997,929 - RR42_RS25210 0.49 -0.1 997,931 + RR42_RS25210 0.49 +0.1 997,932 - RR42_RS25210 0.49 +0.4 997,932 - RR42_RS25210 0.49 +0.1 997,932 - RR42_RS25210 0.49 -0.2 997,998 - RR42_RS25210 0.54 -0.1 998,183 - RR42_RS25210 0.70 +0.5 998,408 - RR42_RS25210 0.89 -0.3 998,473 + -0.2 998,473 + +0.6 998,474 - -0.9 998,537 - -0.5 998,537 - +2.2 998,537 - -3.4 998,537 - +0.9 998,537 - -1.2 998,632 - -0.4 998,665 - -1.0 998,665 - -0.1 998,668 - -1.0 998,668 - -0.5 998,668 - +0.6 998,734 + +0.1 998,756 - RR42_RS25215 0.12 -0.5 998,756 - RR42_RS25215 0.12 -0.4 998,786 + RR42_RS25215 0.17 +0.6 998,787 - RR42_RS25215 0.17 -0.0 998,956 + RR42_RS25215 0.45 -0.4 998,956 + RR42_RS25215 0.45 +0.3 998,957 - RR42_RS25215 0.45 -0.5 998,957 - RR42_RS25215 0.45 -0.8 999,299 + -1.4 999,437 + -0.8 999,531 + +1.4
Or see this region's nucleotide sequence