Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23600 and RR42_RS23605 overlap by 4 nucleotides RR42_RS23605 and RR42_RS23610 are separated by 5 nucleotides RR42_RS23610 and RR42_RS23615 overlap by 4 nucleotides
RR42_RS23600: RR42_RS23600 - SAM-dependent methyltransferase, at 619,682 to 620,965
_RS23600
RR42_RS23605: RR42_RS23605 - CDP-glucose 4,6-dehydratase, at 620,962 to 622,020
_RS23605
RR42_RS23610: RR42_RS23610 - glucose-1-phosphate cytidylyltransferase, at 622,026 to 622,799
_RS23610
RR42_RS23615: RR42_RS23615 - hypothetical protein, at 622,796 to 625,021
_RS23615
Position (kb)
620
621
622
623 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 619.963 kb on - strand, within RR42_RS23600 at 620.250 kb on + strand, within RR42_RS23600 at 620.250 kb on + strand, within RR42_RS23600 at 620.251 kb on - strand, within RR42_RS23600 at 620.256 kb on + strand, within RR42_RS23600 at 620.568 kb on + strand, within RR42_RS23600 at 620.569 kb on - strand, within RR42_RS23600 at 620.655 kb on + strand, within RR42_RS23600 at 620.688 kb on + strand, within RR42_RS23600 at 620.700 kb on + strand, within RR42_RS23600 at 620.707 kb on - strand, within RR42_RS23600 at 620.754 kb on - strand, within RR42_RS23600 at 621.036 kb on - strand at 621.314 kb on + strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.618 kb on - strand, within RR42_RS23605 at 621.911 kb on + strand, within RR42_RS23605 at 621.947 kb on - strand at 621.947 kb on - strand at 622.007 kb on + strand at 622.007 kb on + strand at 622.008 kb on - strand at 622.008 kb on - strand at 622.015 kb on + strand at 622.016 kb on - strand at 622.016 kb on - strand at 622.016 kb on - strand at 622.016 kb on - strand at 622.302 kb on - strand, within RR42_RS23610 at 622.318 kb on + strand, within RR42_RS23610 at 622.319 kb on - strand, within RR42_RS23610 at 622.319 kb on - strand, within RR42_RS23610 at 622.424 kb on + strand, within RR42_RS23610 at 622.425 kb on - strand, within RR42_RS23610 at 622.425 kb on - strand, within RR42_RS23610 at 622.453 kb on + strand, within RR42_RS23610 at 622.589 kb on + strand, within RR42_RS23610 at 622.590 kb on - strand, within RR42_RS23610 at 622.601 kb on - strand, within RR42_RS23610 at 622.613 kb on - strand, within RR42_RS23610 at 622.636 kb on + strand, within RR42_RS23610 at 622.679 kb on - strand, within RR42_RS23610 at 622.974 kb on + strand at 623.003 kb on + strand at 623.003 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 619,963 - RR42_RS23600 0.22 -0.1 620,250 + RR42_RS23600 0.44 -0.4 620,250 + RR42_RS23600 0.44 +0.2 620,251 - RR42_RS23600 0.44 +0.6 620,256 + RR42_RS23600 0.45 +0.1 620,568 + RR42_RS23600 0.69 -0.1 620,569 - RR42_RS23600 0.69 +0.4 620,655 + RR42_RS23600 0.76 +0.5 620,688 + RR42_RS23600 0.78 +0.0 620,700 + RR42_RS23600 0.79 +0.3 620,707 - RR42_RS23600 0.80 +0.0 620,754 - RR42_RS23600 0.83 -0.4 621,036 - +0.8 621,314 + RR42_RS23605 0.33 +0.5 621,555 - RR42_RS23605 0.56 -0.1 621,555 - RR42_RS23605 0.56 +0.9 621,555 - RR42_RS23605 0.56 -0.2 621,618 - RR42_RS23605 0.62 +2.2 621,911 + RR42_RS23605 0.90 +0.6 621,947 - -1.4 621,947 - -0.5 622,007 + +0.0 622,007 + -0.6 622,008 - +0.5 622,008 - +0.4 622,015 + +0.1 622,016 - +1.1 622,016 - +0.0 622,016 - -0.6 622,016 - +2.8 622,302 - RR42_RS23610 0.36 -0.3 622,318 + RR42_RS23610 0.38 -0.3 622,319 - RR42_RS23610 0.38 +0.8 622,319 - RR42_RS23610 0.38 +0.4 622,424 + RR42_RS23610 0.51 -0.0 622,425 - RR42_RS23610 0.52 +0.5 622,425 - RR42_RS23610 0.52 -0.8 622,453 + RR42_RS23610 0.55 -0.5 622,589 + RR42_RS23610 0.73 -0.7 622,590 - RR42_RS23610 0.73 -0.4 622,601 - RR42_RS23610 0.74 -0.8 622,613 - RR42_RS23610 0.76 +3.4 622,636 + RR42_RS23610 0.79 +0.1 622,679 - RR42_RS23610 0.84 -0.6 622,974 + -1.9 623,003 + +0.0 623,003 + +1.8
Or see this region's nucleotide sequence