Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22850 and RR42_RS22855 are separated by 416 nucleotides RR42_RS22855 and RR42_RS22860 are separated by 81 nucleotides RR42_RS22860 and RR42_RS22865 are separated by 57 nucleotides
RR42_RS22850: RR42_RS22850 - amino acid dehydrogenase, at 457,915 to 458,958
_RS22850
RR42_RS22855: RR42_RS22855 - sulfate transporter, at 459,375 to 459,812
_RS22855
RR42_RS22860: RR42_RS22860 - l-threonine 3-dehydrogenase, at 459,894 to 460,949
_RS22860
RR42_RS22865: RR42_RS22865 - 2-amino-3-ketobutyrate CoA ligase, at 461,007 to 462,209
_RS22865
Position (kb) 459
460
461 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 459.030 kb on + strand at 459.112 kb on - strand at 459.195 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.235 kb on - strand at 459.235 kb on - strand at 459.235 kb on - strand at 459.282 kb on + strand at 459.284 kb on + strand at 459.284 kb on + strand at 459.284 kb on + strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.286 kb on + strand at 459.287 kb on - strand at 459.287 kb on - strand at 459.300 kb on + strand at 459.507 kb on + strand, within RR42_RS22855 at 459.507 kb on + strand, within RR42_RS22855 at 459.508 kb on - strand, within RR42_RS22855 at 459.508 kb on - strand, within RR42_RS22855 at 459.513 kb on + strand, within RR42_RS22855 at 459.513 kb on + strand, within RR42_RS22855 at 459.514 kb on - strand, within RR42_RS22855 at 459.646 kb on + strand, within RR42_RS22855 at 459.647 kb on - strand, within RR42_RS22855 at 459.647 kb on - strand, within RR42_RS22855 at 459.661 kb on - strand, within RR42_RS22855 at 459.782 kb on + strand at 459.782 kb on + strand at 459.782 kb on + strand at 459.783 kb on - strand at 459.783 kb on - strand at 459.805 kb on - strand at 459.805 kb on - strand at 459.908 kb on + strand at 460.064 kb on - strand, within RR42_RS22860 at 460.090 kb on + strand, within RR42_RS22860 at 460.090 kb on + strand, within RR42_RS22860 at 460.091 kb on - strand, within RR42_RS22860 at 460.579 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.753 kb on - strand, within RR42_RS22860 at 460.756 kb on + strand, within RR42_RS22860 at 460.757 kb on - strand, within RR42_RS22860 at 460.820 kb on + strand, within RR42_RS22860 at 460.820 kb on + strand, within RR42_RS22860 at 461.079 kb on - strand at 461.153 kb on - strand, within RR42_RS22865 at 461.153 kb on - strand, within RR42_RS22865 at 461.249 kb on + strand, within RR42_RS22865 at 461.250 kb on - strand, within RR42_RS22865 at 461.452 kb on + strand, within RR42_RS22865 at 461.453 kb on - strand, within RR42_RS22865
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 459,030 + -0.1 459,112 - +0.5 459,195 + -0.2 459,234 + -0.6 459,234 + -0.8 459,234 + -0.2 459,234 + +0.6 459,234 + +0.9 459,234 + -0.6 459,234 + +0.6 459,235 - +1.4 459,235 - +1.2 459,235 - -0.8 459,282 + -0.2 459,284 + -0.8 459,284 + -1.6 459,284 + +0.5 459,285 - +0.9 459,285 - +0.4 459,285 - +1.2 459,285 - +1.5 459,285 - +0.3 459,285 - +2.2 459,286 + -2.9 459,287 - +0.9 459,287 - +0.9 459,300 + +0.9 459,507 + RR42_RS22855 0.30 +0.6 459,507 + RR42_RS22855 0.30 +0.4 459,508 - RR42_RS22855 0.30 -0.7 459,508 - RR42_RS22855 0.30 +0.8 459,513 + RR42_RS22855 0.32 +0.1 459,513 + RR42_RS22855 0.32 +0.3 459,514 - RR42_RS22855 0.32 +1.1 459,646 + RR42_RS22855 0.62 +0.9 459,647 - RR42_RS22855 0.62 +1.2 459,647 - RR42_RS22855 0.62 +0.8 459,661 - RR42_RS22855 0.65 +0.8 459,782 + +0.1 459,782 + +0.4 459,782 + +0.8 459,783 - +1.2 459,783 - +0.7 459,805 - +1.0 459,805 - +0.7 459,908 + +0.2 460,064 - RR42_RS22860 0.16 +0.2 460,090 + RR42_RS22860 0.19 +1.2 460,090 + RR42_RS22860 0.19 +0.7 460,091 - RR42_RS22860 0.19 +2.5 460,579 + RR42_RS22860 0.65 +0.1 460,676 + RR42_RS22860 0.74 +1.2 460,676 + RR42_RS22860 0.74 -1.9 460,676 + RR42_RS22860 0.74 -0.2 460,752 + RR42_RS22860 0.81 -0.4 460,752 + RR42_RS22860 0.81 +0.3 460,752 + RR42_RS22860 0.81 -0.2 460,753 - RR42_RS22860 0.81 +0.1 460,756 + RR42_RS22860 0.82 -0.2 460,757 - RR42_RS22860 0.82 -1.1 460,820 + RR42_RS22860 0.88 +1.9 460,820 + RR42_RS22860 0.88 -0.2 461,079 - +1.5 461,153 - RR42_RS22865 0.12 -0.1 461,153 - RR42_RS22865 0.12 +0.0 461,249 + RR42_RS22865 0.20 +0.5 461,250 - RR42_RS22865 0.20 +0.1 461,452 + RR42_RS22865 0.37 -0.1 461,453 - RR42_RS22865 0.37 -0.4
Or see this region's nucleotide sequence