Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15820 and RR42_RS15825 are separated by 662 nucleotides RR42_RS15825 and RR42_RS15830 are separated by 92 nucleotides RR42_RS15830 and RR42_RS15835 are separated by 120 nucleotides
RR42_RS15820: RR42_RS15820 - UDP-glucose 6-dehydrogenase, at 3,418,788 to 3,420,149
_RS15820
RR42_RS15825: RR42_RS15825 - capsular biosynthesis protein, at 3,420,812 to 3,422,728
_RS15825
RR42_RS15830: RR42_RS15830 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 3,422,821 to 3,423,372
_RS15830
RR42_RS15835: RR42_RS15835 - dTDP-4-dehydrorhamnose reductase, at 3,423,493 to 3,424,410
_RS15835
Position (kb)
3420
3421
3422
3423 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3419.857 kb on + strand, within RR42_RS15820 at 3419.858 kb on - strand, within RR42_RS15820 at 3419.858 kb on - strand, within RR42_RS15820 at 3419.907 kb on - strand, within RR42_RS15820 at 3420.010 kb on + strand, within RR42_RS15820 at 3420.011 kb on - strand, within RR42_RS15820 at 3420.011 kb on - strand, within RR42_RS15820 at 3420.011 kb on - strand, within RR42_RS15820 at 3420.011 kb on - strand, within RR42_RS15820 at 3420.015 kb on - strand at 3420.015 kb on - strand at 3420.084 kb on + strand at 3420.084 kb on + strand at 3420.085 kb on - strand at 3420.122 kb on - strand at 3420.122 kb on - strand at 3420.122 kb on - strand at 3420.141 kb on - strand at 3420.162 kb on + strand at 3420.218 kb on + strand at 3420.219 kb on - strand at 3420.219 kb on - strand at 3420.219 kb on - strand at 3420.276 kb on + strand at 3420.323 kb on + strand at 3420.323 kb on + strand at 3420.324 kb on - strand at 3420.324 kb on - strand at 3420.324 kb on - strand at 3420.350 kb on + strand at 3420.351 kb on - strand at 3420.385 kb on - strand at 3420.385 kb on - strand at 3420.585 kb on + strand at 3420.586 kb on - strand at 3420.586 kb on - strand at 3420.598 kb on + strand at 3420.621 kb on + strand at 3420.719 kb on + strand at 3420.719 kb on + strand at 3420.720 kb on - strand at 3420.801 kb on + strand at 3420.802 kb on - strand at 3420.802 kb on - strand at 3420.802 kb on - strand at 3420.802 kb on - strand at 3421.062 kb on + strand, within RR42_RS15825 at 3421.198 kb on + strand, within RR42_RS15825 at 3421.552 kb on + strand, within RR42_RS15825 at 3421.580 kb on + strand, within RR42_RS15825 at 3421.594 kb on + strand, within RR42_RS15825 at 3421.624 kb on - strand, within RR42_RS15825 at 3421.624 kb on - strand, within RR42_RS15825 at 3421.638 kb on + strand, within RR42_RS15825 at 3421.722 kb on - strand, within RR42_RS15825 at 3421.722 kb on - strand, within RR42_RS15825 at 3421.774 kb on - strand, within RR42_RS15825 at 3421.774 kb on - strand, within RR42_RS15825 at 3421.931 kb on + strand, within RR42_RS15825 at 3421.931 kb on + strand, within RR42_RS15825 at 3421.931 kb on + strand, within RR42_RS15825 at 3421.931 kb on + strand, within RR42_RS15825 at 3421.931 kb on + strand, within RR42_RS15825 at 3421.932 kb on - strand, within RR42_RS15825 at 3421.932 kb on - strand, within RR42_RS15825 at 3422.049 kb on - strand, within RR42_RS15825 at 3422.283 kb on + strand, within RR42_RS15825 at 3422.283 kb on + strand, within RR42_RS15825 at 3422.284 kb on - strand, within RR42_RS15825 at 3422.284 kb on - strand, within RR42_RS15825 at 3422.284 kb on - strand, within RR42_RS15825 at 3422.365 kb on + strand, within RR42_RS15825 at 3422.365 kb on + strand, within RR42_RS15825 at 3422.366 kb on - strand, within RR42_RS15825 at 3422.367 kb on + strand, within RR42_RS15825 at 3422.367 kb on + strand, within RR42_RS15825 at 3422.368 kb on - strand, within RR42_RS15825 at 3422.368 kb on - strand, within RR42_RS15825 at 3422.368 kb on - strand, within RR42_RS15825 at 3422.388 kb on + strand, within RR42_RS15825 at 3422.389 kb on - strand, within RR42_RS15825 at 3422.508 kb on + strand, within RR42_RS15825 at 3422.508 kb on + strand, within RR42_RS15825 at 3422.508 kb on + strand, within RR42_RS15825 at 3422.508 kb on + strand, within RR42_RS15825 at 3422.508 kb on + strand, within RR42_RS15825 at 3422.527 kb on - strand, within RR42_RS15825 at 3422.741 kb on - strand at 3422.756 kb on + strand at 3422.822 kb on + strand at 3423.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 3,419,857 + RR42_RS15820 0.78 +2.5 3,419,858 - RR42_RS15820 0.79 +0.4 3,419,858 - RR42_RS15820 0.79 -1.0 3,419,907 - RR42_RS15820 0.82 -0.1 3,420,010 + RR42_RS15820 0.90 +1.7 3,420,011 - RR42_RS15820 0.90 -0.0 3,420,011 - RR42_RS15820 0.90 +0.1 3,420,011 - RR42_RS15820 0.90 +0.0 3,420,011 - RR42_RS15820 0.90 -0.8 3,420,015 - -1.6 3,420,015 - -2.3 3,420,084 + +1.2 3,420,084 + +1.0 3,420,085 - +1.3 3,420,122 - -0.7 3,420,122 - +0.4 3,420,122 - -0.0 3,420,141 - +0.3 3,420,162 + +0.2 3,420,218 + +0.1 3,420,219 - -1.1 3,420,219 - +0.8 3,420,219 - -0.4 3,420,276 + -0.0 3,420,323 + +0.1 3,420,323 + -2.7 3,420,324 - -0.9 3,420,324 - -0.3 3,420,324 - +0.4 3,420,350 + +0.4 3,420,351 - -0.0 3,420,385 - +0.8 3,420,385 - -1.1 3,420,585 + -0.3 3,420,586 - -0.6 3,420,586 - +0.4 3,420,598 + +0.5 3,420,621 + +0.7 3,420,719 + -1.2 3,420,719 + +0.2 3,420,720 - -0.3 3,420,801 + -0.3 3,420,802 - +0.0 3,420,802 - +0.8 3,420,802 - -0.1 3,420,802 - +0.1 3,421,062 + RR42_RS15825 0.13 -0.5 3,421,198 + RR42_RS15825 0.20 -0.6 3,421,552 + RR42_RS15825 0.39 +0.7 3,421,580 + RR42_RS15825 0.40 -0.2 3,421,594 + RR42_RS15825 0.41 +0.4 3,421,624 - RR42_RS15825 0.42 +0.8 3,421,624 - RR42_RS15825 0.42 +1.1 3,421,638 + RR42_RS15825 0.43 +1.2 3,421,722 - RR42_RS15825 0.47 +0.7 3,421,722 - RR42_RS15825 0.47 +0.4 3,421,774 - RR42_RS15825 0.50 +0.7 3,421,774 - RR42_RS15825 0.50 +1.1 3,421,931 + RR42_RS15825 0.58 +0.7 3,421,931 + RR42_RS15825 0.58 +1.2 3,421,931 + RR42_RS15825 0.58 +2.2 3,421,931 + RR42_RS15825 0.58 +0.4 3,421,931 + RR42_RS15825 0.58 -0.0 3,421,932 - RR42_RS15825 0.58 +0.8 3,421,932 - RR42_RS15825 0.58 -0.2 3,422,049 - RR42_RS15825 0.65 +0.5 3,422,283 + RR42_RS15825 0.77 -0.3 3,422,283 + RR42_RS15825 0.77 +1.1 3,422,284 - RR42_RS15825 0.77 +0.3 3,422,284 - RR42_RS15825 0.77 +1.4 3,422,284 - RR42_RS15825 0.77 +0.3 3,422,365 + RR42_RS15825 0.81 +0.2 3,422,365 + RR42_RS15825 0.81 +0.8 3,422,366 - RR42_RS15825 0.81 -2.7 3,422,367 + RR42_RS15825 0.81 +2.8 3,422,367 + RR42_RS15825 0.81 -0.2 3,422,368 - RR42_RS15825 0.81 -0.2 3,422,368 - RR42_RS15825 0.81 +0.1 3,422,368 - RR42_RS15825 0.81 +0.9 3,422,388 + RR42_RS15825 0.82 +0.3 3,422,389 - RR42_RS15825 0.82 +0.9 3,422,508 + RR42_RS15825 0.88 -0.6 3,422,508 + RR42_RS15825 0.88 -0.3 3,422,508 + RR42_RS15825 0.88 +1.2 3,422,508 + RR42_RS15825 0.88 +0.3 3,422,508 + RR42_RS15825 0.88 +0.5 3,422,527 - RR42_RS15825 0.89 +2.5 3,422,741 - +3.6 3,422,756 + -0.3 3,422,822 + -0.5 3,423,373 - -1.5
Or see this region's nucleotide sequence