Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11000 and RR42_RS11005 are separated by 316 nucleotides RR42_RS11005 and RR42_RS11010 are separated by 80 nucleotides RR42_RS11010 and RR42_RS11015 are separated by 141 nucleotides
RR42_RS11000: RR42_RS11000 - alpha/beta hydrolase, at 2,349,045 to 2,350,010
_RS11000
RR42_RS11005: RR42_RS11005 - 4-hydroxybutyrate dehydrogenase, at 2,350,327 to 2,351,475
_RS11005
RR42_RS11010: RR42_RS11010 - macro domain-containing protein, at 2,351,556 to 2,352,077
_RS11010
RR42_RS11015: RR42_RS11015 - hypothetical protein, at 2,352,219 to 2,352,500
_RS11015
Position (kb)
2350
2351
2352 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2349.341 kb on - strand, within RR42_RS11000 at 2349.341 kb on - strand, within RR42_RS11000 at 2349.382 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.646 kb on + strand, within RR42_RS11000 at 2349.749 kb on - strand, within RR42_RS11000 at 2349.796 kb on + strand, within RR42_RS11000 at 2349.797 kb on - strand, within RR42_RS11000 at 2350.175 kb on + strand at 2350.176 kb on - strand at 2350.206 kb on - strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.509 kb on - strand, within RR42_RS11005 at 2350.672 kb on + strand, within RR42_RS11005 at 2350.672 kb on + strand, within RR42_RS11005 at 2350.673 kb on - strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2351.001 kb on + strand, within RR42_RS11005 at 2351.002 kb on - strand, within RR42_RS11005 at 2351.473 kb on + strand at 2351.473 kb on + strand at 2351.473 kb on + strand at 2351.473 kb on + strand at 2351.473 kb on + strand at 2351.474 kb on - strand at 2351.787 kb on + strand, within RR42_RS11010 at 2351.787 kb on + strand, within RR42_RS11010 at 2351.787 kb on + strand, within RR42_RS11010 at 2351.788 kb on - strand, within RR42_RS11010 at 2351.859 kb on + strand, within RR42_RS11010 at 2351.918 kb on + strand, within RR42_RS11010 at 2351.919 kb on - strand, within RR42_RS11010 at 2351.934 kb on + strand, within RR42_RS11010 at 2351.934 kb on + strand, within RR42_RS11010 at 2351.934 kb on + strand, within RR42_RS11010 at 2351.935 kb on - strand, within RR42_RS11010 at 2351.935 kb on - strand, within RR42_RS11010 at 2351.964 kb on - strand, within RR42_RS11010 at 2351.964 kb on - strand, within RR42_RS11010 at 2352.212 kb on - strand at 2352.216 kb on + strand at 2352.216 kb on + strand at 2352.216 kb on + strand at 2352.216 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 2,349,341 - RR42_RS11000 0.31 -1.1 2,349,341 - RR42_RS11000 0.31 +0.4 2,349,382 + RR42_RS11000 0.35 -0.3 2,349,439 + RR42_RS11000 0.41 -1.1 2,349,439 + RR42_RS11000 0.41 +1.4 2,349,439 + RR42_RS11000 0.41 +0.3 2,349,440 - RR42_RS11000 0.41 -0.4 2,349,440 - RR42_RS11000 0.41 +1.3 2,349,440 - RR42_RS11000 0.41 +0.6 2,349,440 - RR42_RS11000 0.41 -0.1 2,349,646 + RR42_RS11000 0.62 +0.2 2,349,749 - RR42_RS11000 0.73 -0.1 2,349,796 + RR42_RS11000 0.78 -0.8 2,349,797 - RR42_RS11000 0.78 +1.2 2,350,175 + +0.4 2,350,176 - +0.3 2,350,206 - +0.2 2,350,324 + -1.8 2,350,324 + +1.0 2,350,324 + -1.1 2,350,324 + +1.0 2,350,324 + -0.9 2,350,324 + +0.5 2,350,325 - -0.1 2,350,325 - +0.7 2,350,325 - -1.2 2,350,325 - +1.0 2,350,325 - +0.9 2,350,325 - +0.2 2,350,325 - +1.1 2,350,509 - RR42_RS11005 0.16 +1.1 2,350,672 + RR42_RS11005 0.30 +0.7 2,350,672 + RR42_RS11005 0.30 -1.4 2,350,673 - RR42_RS11005 0.30 -1.6 2,350,844 + RR42_RS11005 0.45 +1.3 2,350,844 + RR42_RS11005 0.45 -0.4 2,350,844 + RR42_RS11005 0.45 -0.4 2,350,844 + RR42_RS11005 0.45 +0.4 2,351,001 + RR42_RS11005 0.59 -0.1 2,351,002 - RR42_RS11005 0.59 +0.2 2,351,473 + -1.0 2,351,473 + -0.5 2,351,473 + +1.1 2,351,473 + -0.9 2,351,473 + +1.0 2,351,474 - -1.6 2,351,787 + RR42_RS11010 0.44 +0.1 2,351,787 + RR42_RS11010 0.44 -0.8 2,351,787 + RR42_RS11010 0.44 +0.9 2,351,788 - RR42_RS11010 0.44 -2.9 2,351,859 + RR42_RS11010 0.58 +1.0 2,351,918 + RR42_RS11010 0.69 -0.4 2,351,919 - RR42_RS11010 0.70 -0.0 2,351,934 + RR42_RS11010 0.72 -0.0 2,351,934 + RR42_RS11010 0.72 -0.2 2,351,934 + RR42_RS11010 0.72 -0.5 2,351,935 - RR42_RS11010 0.73 -1.4 2,351,935 - RR42_RS11010 0.73 +2.3 2,351,964 - RR42_RS11010 0.78 +0.6 2,351,964 - RR42_RS11010 0.78 +0.4 2,352,212 - +0.2 2,352,216 + +0.1 2,352,216 + -0.6 2,352,216 + -1.5 2,352,216 + +0.6
Or see this region's nucleotide sequence