Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05435

Experiment: L-Valine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05425 and RR42_RS05430 overlap by 4 nucleotidesRR42_RS05430 and RR42_RS05435 are separated by 368 nucleotidesRR42_RS05435 and RR42_RS05440 overlap by 1 nucleotidesRR42_RS05440 and RR42_RS05445 are separated by 97 nucleotides RR42_RS05425: RR42_RS05425 - membrane protein, at 1,197,855 to 1,198,289 _RS05425 RR42_RS05430: RR42_RS05430 - DNA-directed RNA polymerase sigma-70 factor, at 1,198,286 to 1,198,852 _RS05430 RR42_RS05435: RR42_RS05435 - acetolactate synthase 3 catalytic subunit, at 1,199,221 to 1,200,981 _RS05435 RR42_RS05440: RR42_RS05440 - acetolactate synthase, at 1,200,981 to 1,201,472 _RS05440 RR42_RS05445: RR42_RS05445 - ketol-acid reductoisomerase, at 1,201,570 to 1,202,586 _RS05445 Position (kb) 1199 1200 1201Strain fitness (log2 ratio) -3 -2 -1 0 1at 1198.406 kb on - strand, within RR42_RS05430at 1198.567 kb on + strand, within RR42_RS05430at 1198.619 kb on - strand, within RR42_RS05430at 1198.626 kb on - strand, within RR42_RS05430at 1198.667 kb on + strand, within RR42_RS05430at 1198.668 kb on - strand, within RR42_RS05430at 1198.832 kb on + strandat 1198.832 kb on + strandat 1198.901 kb on - strandat 1198.934 kb on + strandat 1198.934 kb on + strandat 1198.935 kb on - strandat 1198.941 kb on + strandat 1198.941 kb on + strandat 1198.942 kb on - strandat 1199.130 kb on + strandat 1199.130 kb on + strandat 1199.130 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (N)
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1,198,406 - RR42_RS05430 0.21 +0.6
1,198,567 + RR42_RS05430 0.50 -0.0
1,198,619 - RR42_RS05430 0.59 -1.9
1,198,626 - RR42_RS05430 0.60 +0.4
1,198,667 + RR42_RS05430 0.67 +0.5
1,198,668 - RR42_RS05430 0.67 -2.8
1,198,832 + -1.7
1,198,832 + -1.1
1,198,901 - -1.3
1,198,934 + -1.7
1,198,934 + -1.1
1,198,935 - -1.1
1,198,941 + -0.1
1,198,941 + +0.6
1,198,942 - -0.2
1,199,130 + +0.7
1,199,130 + +0.1
1,199,130 + +1.0

Or see this region's nucleotide sequence