Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05425 and RR42_RS05430 overlap by 4 nucleotides RR42_RS05430 and RR42_RS05435 are separated by 368 nucleotides RR42_RS05435 and RR42_RS05440 overlap by 1 nucleotides RR42_RS05440 and RR42_RS05445 are separated by 97 nucleotides
RR42_RS05425: RR42_RS05425 - membrane protein, at 1,197,855 to 1,198,289
_RS05425
RR42_RS05430: RR42_RS05430 - DNA-directed RNA polymerase sigma-70 factor, at 1,198,286 to 1,198,852
_RS05430
RR42_RS05435: RR42_RS05435 - acetolactate synthase 3 catalytic subunit, at 1,199,221 to 1,200,981
_RS05435
RR42_RS05440: RR42_RS05440 - acetolactate synthase, at 1,200,981 to 1,201,472
_RS05440
RR42_RS05445: RR42_RS05445 - ketol-acid reductoisomerase, at 1,201,570 to 1,202,586
_RS05445
Position (kb)
1199
1200
1201 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1198.406 kb on - strand, within RR42_RS05430 at 1198.567 kb on + strand, within RR42_RS05430 at 1198.619 kb on - strand, within RR42_RS05430 at 1198.626 kb on - strand, within RR42_RS05430 at 1198.667 kb on + strand, within RR42_RS05430 at 1198.668 kb on - strand, within RR42_RS05430 at 1198.832 kb on + strand at 1198.832 kb on + strand at 1198.901 kb on - strand at 1198.934 kb on + strand at 1198.934 kb on + strand at 1198.935 kb on - strand at 1198.941 kb on + strand at 1198.941 kb on + strand at 1198.942 kb on - strand at 1199.130 kb on + strand at 1199.130 kb on + strand at 1199.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 1,198,406 - RR42_RS05430 0.21 +0.6 1,198,567 + RR42_RS05430 0.50 -0.0 1,198,619 - RR42_RS05430 0.59 -1.9 1,198,626 - RR42_RS05430 0.60 +0.4 1,198,667 + RR42_RS05430 0.67 +0.5 1,198,668 - RR42_RS05430 0.67 -2.8 1,198,832 + -1.7 1,198,832 + -1.1 1,198,901 - -1.3 1,198,934 + -1.7 1,198,934 + -1.1 1,198,935 - -1.1 1,198,941 + -0.1 1,198,941 + +0.6 1,198,942 - -0.2 1,199,130 + +0.7 1,199,130 + +0.1 1,199,130 + +1.0
Or see this region's nucleotide sequence