Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04470 and RR42_RS04475 are separated by 93 nucleotides RR42_RS04475 and RR42_RS04480 are separated by 0 nucleotides RR42_RS04480 and RR42_RS04485 are separated by 134 nucleotides
RR42_RS04470: RR42_RS04470 - prephenate dehydrogenase, at 999,499 to 1,000,407
_RS04470
RR42_RS04475: RR42_RS04475 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,000,501 to 1,001,811
_RS04475
RR42_RS04480: RR42_RS04480 - membrane protein, at 1,001,812 to 1,002,045
_RS04480
RR42_RS04485: RR42_RS04485 - cytidylate kinase, at 1,002,180 to 1,002,842
_RS04485
Position (kb) 1000
1001
1002 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 999.787 kb on - strand at 999.934 kb on + strand, within RR42_RS04470 at 999.934 kb on + strand, within RR42_RS04470 at 999.934 kb on + strand, within RR42_RS04470 at 999.934 kb on + strand, within RR42_RS04470 at 999.935 kb on - strand, within RR42_RS04470 at 999.935 kb on - strand, within RR42_RS04470 at 999.935 kb on - strand, within RR42_RS04470 at 1000.267 kb on + strand, within RR42_RS04470 at 1000.267 kb on + strand, within RR42_RS04470 at 1000.268 kb on - strand, within RR42_RS04470 at 1000.332 kb on + strand at 1000.332 kb on + strand at 1000.333 kb on - strand at 1000.488 kb on + strand at 1000.489 kb on - strand at 1000.489 kb on - strand at 1000.828 kb on + strand, within RR42_RS04475 at 1000.829 kb on - strand, within RR42_RS04475 at 1000.851 kb on + strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.898 kb on + strand, within RR42_RS04475 at 1000.898 kb on + strand, within RR42_RS04475 at 1000.915 kb on + strand, within RR42_RS04475 at 1001.104 kb on + strand, within RR42_RS04475 at 1001.248 kb on + strand, within RR42_RS04475 at 1001.249 kb on - strand, within RR42_RS04475 at 1001.347 kb on - strand, within RR42_RS04475 at 1001.432 kb on + strand, within RR42_RS04475 at 1001.451 kb on + strand, within RR42_RS04475 at 1001.506 kb on - strand, within RR42_RS04475 at 1001.615 kb on - strand, within RR42_RS04475 at 1001.677 kb on + strand, within RR42_RS04475 at 1001.678 kb on - strand, within RR42_RS04475 at 1001.779 kb on + strand at 1001.817 kb on + strand at 1001.818 kb on - strand at 1001.912 kb on + strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.105 kb on + strand at 1002.116 kb on + strand at 1002.117 kb on - strand at 1002.171 kb on + strand at 1002.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 999,787 - -0.4 999,934 + RR42_RS04470 0.48 -2.3 999,934 + RR42_RS04470 0.48 -1.2 999,934 + RR42_RS04470 0.48 -0.9 999,934 + RR42_RS04470 0.48 -2.1 999,935 - RR42_RS04470 0.48 -1.1 999,935 - RR42_RS04470 0.48 -1.4 999,935 - RR42_RS04470 0.48 -5.6 1,000,267 + RR42_RS04470 0.84 -0.1 1,000,267 + RR42_RS04470 0.84 -0.5 1,000,268 - RR42_RS04470 0.85 -3.1 1,000,332 + -0.5 1,000,332 + -0.1 1,000,333 - -5.1 1,000,488 + +0.5 1,000,489 - -4.8 1,000,489 - -4.1 1,000,828 + RR42_RS04475 0.25 -4.5 1,000,829 - RR42_RS04475 0.25 -2.0 1,000,851 + RR42_RS04475 0.27 -4.7 1,000,852 - RR42_RS04475 0.27 -1.4 1,000,852 - RR42_RS04475 0.27 -5.5 1,000,852 - RR42_RS04475 0.27 -5.0 1,000,852 - RR42_RS04475 0.27 -3.9 1,000,898 + RR42_RS04475 0.30 -2.9 1,000,898 + RR42_RS04475 0.30 -3.2 1,000,915 + RR42_RS04475 0.32 -5.8 1,001,104 + RR42_RS04475 0.46 -5.9 1,001,248 + RR42_RS04475 0.57 -1.7 1,001,249 - RR42_RS04475 0.57 -2.9 1,001,347 - RR42_RS04475 0.65 +0.2 1,001,432 + RR42_RS04475 0.71 -2.8 1,001,451 + RR42_RS04475 0.72 -5.1 1,001,506 - RR42_RS04475 0.77 -4.5 1,001,615 - RR42_RS04475 0.85 -1.4 1,001,677 + RR42_RS04475 0.90 -1.8 1,001,678 - RR42_RS04475 0.90 -1.7 1,001,779 + -2.4 1,001,817 + -0.1 1,001,818 - -0.5 1,001,912 + RR42_RS04480 0.43 -1.1 1,001,913 - RR42_RS04480 0.43 +0.1 1,001,913 - RR42_RS04480 0.43 +0.6 1,001,913 - RR42_RS04480 0.43 -0.3 1,001,913 - RR42_RS04480 0.43 +0.7 1,001,913 - RR42_RS04480 0.43 +0.2 1,001,913 - RR42_RS04480 0.43 +0.1 1,001,987 + RR42_RS04480 0.75 +0.4 1,001,987 + RR42_RS04480 0.75 +0.6 1,001,987 + RR42_RS04480 0.75 -1.0 1,001,987 + RR42_RS04480 0.75 +2.8 1,001,987 + RR42_RS04480 0.75 +0.4 1,001,988 - RR42_RS04480 0.75 +0.1 1,001,988 - RR42_RS04480 0.75 -0.1 1,001,988 - RR42_RS04480 0.75 -0.4 1,001,988 - RR42_RS04480 0.75 +0.5 1,002,048 + -0.4 1,002,048 + -0.4 1,002,048 + -0.2 1,002,048 + -0.2 1,002,048 + -1.2 1,002,105 + -1.1 1,002,116 + +1.1 1,002,117 - +0.6 1,002,171 + -0.3 1,002,185 + +0.2
Or see this region's nucleotide sequence