Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00920 and RR42_RS00925 are separated by 273 nucleotides RR42_RS00925 and RR42_RS00930 overlap by 11 nucleotides
RR42_RS00920: RR42_RS00920 - dienelactone hydrolase, at 219,622 to 220,527
_RS00920
RR42_RS00925: RR42_RS00925 - adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA, at 220,801 to 222,288
_RS00925
RR42_RS00930: RR42_RS00930 - 8-amino-7-oxononanoate synthase, at 222,278 to 223,495
_RS00930
Position (kb)
220
221
222
223 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 219.950 kb on - strand, within RR42_RS00920 at 220.072 kb on + strand, within RR42_RS00920 at 220.072 kb on + strand, within RR42_RS00920 at 220.072 kb on + strand, within RR42_RS00920 at 220.072 kb on + strand, within RR42_RS00920 at 220.073 kb on - strand, within RR42_RS00920 at 220.073 kb on - strand, within RR42_RS00920 at 220.073 kb on - strand, within RR42_RS00920 at 220.073 kb on - strand, within RR42_RS00920 at 220.073 kb on - strand, within RR42_RS00920 at 220.090 kb on + strand, within RR42_RS00920 at 220.090 kb on + strand, within RR42_RS00920 at 220.091 kb on - strand, within RR42_RS00920 at 220.091 kb on - strand, within RR42_RS00920 at 220.091 kb on - strand, within RR42_RS00920 at 220.168 kb on + strand, within RR42_RS00920 at 220.169 kb on - strand, within RR42_RS00920 at 220.318 kb on + strand, within RR42_RS00920 at 220.318 kb on + strand, within RR42_RS00920 at 220.318 kb on + strand, within RR42_RS00920 at 220.319 kb on - strand, within RR42_RS00920 at 220.358 kb on - strand, within RR42_RS00920 at 220.358 kb on - strand, within RR42_RS00920 at 220.405 kb on + strand, within RR42_RS00920 at 220.431 kb on + strand, within RR42_RS00920 at 220.431 kb on + strand, within RR42_RS00920 at 220.431 kb on + strand, within RR42_RS00920 at 220.431 kb on + strand, within RR42_RS00920 at 220.432 kb on - strand, within RR42_RS00920 at 220.699 kb on + strand at 220.699 kb on + strand at 220.700 kb on - strand at 220.700 kb on - strand at 220.987 kb on + strand, within RR42_RS00925 at 220.988 kb on - strand, within RR42_RS00925 at 221.288 kb on - strand, within RR42_RS00925 at 221.348 kb on - strand, within RR42_RS00925 at 221.348 kb on - strand, within RR42_RS00925 at 221.348 kb on - strand, within RR42_RS00925 at 221.851 kb on + strand, within RR42_RS00925 at 221.851 kb on + strand, within RR42_RS00925 at 222.000 kb on - strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.044 kb on - strand, within RR42_RS00925 at 222.133 kb on + strand, within RR42_RS00925 at 222.133 kb on + strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.149 kb on - strand at 222.149 kb on - strand at 222.149 kb on - strand at 222.272 kb on + strand at 222.273 kb on - strand at 222.273 kb on - strand at 222.353 kb on + strand at 222.354 kb on - strand at 222.614 kb on + strand, within RR42_RS00930 at 222.629 kb on + strand, within RR42_RS00930 at 222.629 kb on + strand, within RR42_RS00930 at 222.629 kb on + strand, within RR42_RS00930 at 222.631 kb on + strand, within RR42_RS00930 at 222.631 kb on + strand, within RR42_RS00930 at 222.632 kb on - strand, within RR42_RS00930 at 222.632 kb on - strand, within RR42_RS00930 at 222.632 kb on - strand, within RR42_RS00930 at 222.661 kb on - strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 223.094 kb on + strand, within RR42_RS00930 at 223.094 kb on + strand, within RR42_RS00930 at 223.094 kb on + strand, within RR42_RS00930 at 223.095 kb on - strand, within RR42_RS00930 at 223.100 kb on + strand, within RR42_RS00930 at 223.100 kb on + strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 219,950 - RR42_RS00920 0.36 +0.4 220,072 + RR42_RS00920 0.50 +2.3 220,072 + RR42_RS00920 0.50 -0.6 220,072 + RR42_RS00920 0.50 +0.6 220,072 + RR42_RS00920 0.50 -1.7 220,073 - RR42_RS00920 0.50 -0.8 220,073 - RR42_RS00920 0.50 +0.2 220,073 - RR42_RS00920 0.50 -2.0 220,073 - RR42_RS00920 0.50 -0.2 220,073 - RR42_RS00920 0.50 -0.3 220,090 + RR42_RS00920 0.52 -1.4 220,090 + RR42_RS00920 0.52 -0.2 220,091 - RR42_RS00920 0.52 -1.4 220,091 - RR42_RS00920 0.52 +0.2 220,091 - RR42_RS00920 0.52 +0.8 220,168 + RR42_RS00920 0.60 -1.1 220,169 - RR42_RS00920 0.60 -0.3 220,318 + RR42_RS00920 0.77 +0.2 220,318 + RR42_RS00920 0.77 -0.8 220,318 + RR42_RS00920 0.77 -0.2 220,319 - RR42_RS00920 0.77 -1.7 220,358 - RR42_RS00920 0.81 -0.6 220,358 - RR42_RS00920 0.81 -0.1 220,405 + RR42_RS00920 0.86 +0.4 220,431 + RR42_RS00920 0.89 -0.9 220,431 + RR42_RS00920 0.89 -0.1 220,431 + RR42_RS00920 0.89 -0.9 220,431 + RR42_RS00920 0.89 +0.5 220,432 - RR42_RS00920 0.89 -0.4 220,699 + +0.4 220,699 + -0.1 220,700 - -1.3 220,700 - -2.2 220,987 + RR42_RS00925 0.12 +0.1 220,988 - RR42_RS00925 0.13 -1.4 221,288 - RR42_RS00925 0.33 -1.0 221,348 - RR42_RS00925 0.37 -0.1 221,348 - RR42_RS00925 0.37 -2.4 221,348 - RR42_RS00925 0.37 -2.1 221,851 + RR42_RS00925 0.71 -1.6 221,851 + RR42_RS00925 0.71 -0.4 222,000 - RR42_RS00925 0.81 -1.7 222,043 + RR42_RS00925 0.83 +0.4 222,043 + RR42_RS00925 0.83 +0.0 222,043 + RR42_RS00925 0.83 -0.7 222,043 + RR42_RS00925 0.83 -0.3 222,044 - RR42_RS00925 0.84 -2.0 222,133 + RR42_RS00925 0.90 -0.3 222,133 + RR42_RS00925 0.90 +1.2 222,134 - RR42_RS00925 0.90 -2.0 222,134 - RR42_RS00925 0.90 -2.7 222,134 - RR42_RS00925 0.90 -0.1 222,134 - RR42_RS00925 0.90 -2.3 222,134 - RR42_RS00925 0.90 -1.0 222,134 - RR42_RS00925 0.90 -2.0 222,149 - +0.4 222,149 - -0.9 222,149 - -0.2 222,272 + +0.7 222,273 - -1.0 222,273 - -1.0 222,353 + -0.7 222,354 - +0.5 222,614 + RR42_RS00930 0.28 +0.8 222,629 + RR42_RS00930 0.29 +0.2 222,629 + RR42_RS00930 0.29 -0.2 222,629 + RR42_RS00930 0.29 +0.4 222,631 + RR42_RS00930 0.29 -0.0 222,631 + RR42_RS00930 0.29 -0.1 222,632 - RR42_RS00930 0.29 -1.0 222,632 - RR42_RS00930 0.29 -1.7 222,632 - RR42_RS00930 0.29 -2.0 222,661 - RR42_RS00930 0.31 -0.8 222,998 + RR42_RS00930 0.59 +0.1 222,998 + RR42_RS00930 0.59 -0.7 222,998 + RR42_RS00930 0.59 +0.0 222,998 + RR42_RS00930 0.59 -0.3 222,999 - RR42_RS00930 0.59 +0.3 222,999 - RR42_RS00930 0.59 -2.1 222,999 - RR42_RS00930 0.59 -0.8 222,999 - RR42_RS00930 0.59 -1.3 222,999 - RR42_RS00930 0.59 +0.2 222,999 - RR42_RS00930 0.59 -1.7 222,999 - RR42_RS00930 0.59 -0.7 222,999 - RR42_RS00930 0.59 -1.6 222,999 - RR42_RS00930 0.59 +1.3 222,999 - RR42_RS00930 0.59 -1.6 223,094 + RR42_RS00930 0.67 +0.4 223,094 + RR42_RS00930 0.67 -0.8 223,094 + RR42_RS00930 0.67 +0.1 223,095 - RR42_RS00930 0.67 -0.3 223,100 + RR42_RS00930 0.67 +0.1 223,100 + RR42_RS00930 0.67 -1.3 223,101 - RR42_RS00930 0.68 -0.9 223,101 - RR42_RS00930 0.68 -0.8 223,101 - RR42_RS00930 0.68 -2.0 223,101 - RR42_RS00930 0.68 -1.5 223,101 - RR42_RS00930 0.68 -0.8 223,101 - RR42_RS00930 0.68 -1.3
Or see this region's nucleotide sequence