Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14270 and EX31_RS14275 are separated by 143 nucleotides EX31_RS14275 and EX31_RS14280 are separated by 56 nucleotides
EX31_RS14270: EX31_RS14270 - glycerol kinase GlpK, at 35,551 to 37,074
_RS14270
EX31_RS14275: EX31_RS14275 - class II fructose-bisphosphatase, at 37,218 to 38,228
_RS14275
EX31_RS14280: EX31_RS14280 - multidrug efflux MFS transporter EmrD, at 38,285 to 39,478
_RS14280
Position (kb) 37
38
39 Strain fitness (log2 ratio) -2
-1
0
1
2 at 36.239 kb on - strand, within EX31_RS14270 at 36.239 kb on - strand, within EX31_RS14270 at 36.261 kb on + strand, within EX31_RS14270 at 36.262 kb on - strand, within EX31_RS14270 at 36.276 kb on + strand, within EX31_RS14270 at 36.283 kb on - strand, within EX31_RS14270 at 36.283 kb on - strand, within EX31_RS14270 at 36.319 kb on + strand, within EX31_RS14270 at 36.320 kb on - strand, within EX31_RS14270 at 36.320 kb on - strand, within EX31_RS14270 at 36.320 kb on - strand, within EX31_RS14270 at 36.335 kb on + strand, within EX31_RS14270 at 36.336 kb on - strand, within EX31_RS14270 at 36.336 kb on - strand, within EX31_RS14270 at 36.336 kb on - strand, within EX31_RS14270 at 36.361 kb on + strand, within EX31_RS14270 at 36.361 kb on + strand, within EX31_RS14270 at 36.362 kb on - strand, within EX31_RS14270 at 36.362 kb on - strand, within EX31_RS14270 at 36.362 kb on - strand, within EX31_RS14270 at 36.466 kb on + strand, within EX31_RS14270 at 36.466 kb on + strand, within EX31_RS14270 at 36.485 kb on + strand, within EX31_RS14270 at 36.485 kb on + strand, within EX31_RS14270 at 36.485 kb on + strand, within EX31_RS14270 at 36.486 kb on - strand, within EX31_RS14270 at 36.559 kb on + strand, within EX31_RS14270 at 36.595 kb on + strand, within EX31_RS14270 at 36.596 kb on - strand, within EX31_RS14270 at 36.599 kb on + strand, within EX31_RS14270 at 36.599 kb on + strand, within EX31_RS14270 at 36.600 kb on - strand, within EX31_RS14270 at 36.600 kb on - strand, within EX31_RS14270 at 36.644 kb on - strand, within EX31_RS14270 at 36.725 kb on - strand, within EX31_RS14270 at 36.741 kb on - strand, within EX31_RS14270 at 36.741 kb on - strand, within EX31_RS14270 at 36.741 kb on - strand, within EX31_RS14270 at 36.741 kb on - strand, within EX31_RS14270 at 37.005 kb on + strand at 37.005 kb on + strand at 37.005 kb on + strand at 37.005 kb on + strand at 37.005 kb on + strand at 37.006 kb on - strand at 37.006 kb on - strand at 37.006 kb on - strand at 37.006 kb on - strand at 37.006 kb on - strand at 37.006 kb on - strand at 37.006 kb on - strand at 37.015 kb on + strand at 37.015 kb on + strand at 37.017 kb on + strand at 37.017 kb on + strand at 37.018 kb on - strand at 37.018 kb on - strand at 37.072 kb on + strand at 37.073 kb on - strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.083 kb on + strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.084 kb on - strand at 37.153 kb on + strand at 37.205 kb on + strand at 37.206 kb on - strand at 37.231 kb on + strand at 37.231 kb on + strand at 37.232 kb on - strand at 37.232 kb on - strand at 37.232 kb on - strand at 37.279 kb on - strand at 37.279 kb on - strand at 37.343 kb on + strand, within EX31_RS14275 at 37.343 kb on + strand, within EX31_RS14275 at 37.344 kb on - strand, within EX31_RS14275 at 37.352 kb on + strand, within EX31_RS14275 at 37.370 kb on + strand, within EX31_RS14275 at 37.370 kb on + strand, within EX31_RS14275 at 37.371 kb on - strand, within EX31_RS14275 at 37.373 kb on + strand, within EX31_RS14275 at 37.373 kb on + strand, within EX31_RS14275 at 37.373 kb on + strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.374 kb on - strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.416 kb on + strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.417 kb on - strand, within EX31_RS14275 at 37.418 kb on + strand, within EX31_RS14275 at 37.419 kb on - strand, within EX31_RS14275 at 37.419 kb on - strand, within EX31_RS14275 at 37.475 kb on + strand, within EX31_RS14275 at 37.476 kb on - strand, within EX31_RS14275 at 37.484 kb on + strand, within EX31_RS14275 at 37.485 kb on - strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.572 kb on + strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.573 kb on - strand, within EX31_RS14275 at 37.617 kb on - strand, within EX31_RS14275 at 37.658 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.693 kb on + strand, within EX31_RS14275 at 37.694 kb on - strand, within EX31_RS14275 at 37.694 kb on - strand, within EX31_RS14275 at 37.694 kb on - strand, within EX31_RS14275 at 37.694 kb on - strand, within EX31_RS14275 at 37.833 kb on + strand, within EX31_RS14275 at 37.833 kb on + strand, within EX31_RS14275 at 37.833 kb on + strand, within EX31_RS14275 at 37.833 kb on + strand, within EX31_RS14275 at 37.941 kb on - strand, within EX31_RS14275 at 38.030 kb on + strand, within EX31_RS14275 at 38.031 kb on - strand, within EX31_RS14275 at 38.070 kb on - strand, within EX31_RS14275 at 38.072 kb on + strand, within EX31_RS14275 at 38.184 kb on + strand at 38.184 kb on + strand at 38.185 kb on - strand at 38.219 kb on + strand at 38.220 kb on - strand at 38.220 kb on - strand at 38.220 kb on - strand at 38.220 kb on - strand at 38.222 kb on + strand at 38.223 kb on - strand at 38.293 kb on + strand at 38.293 kb on + strand at 38.293 kb on + strand at 38.293 kb on + strand at 38.294 kb on - strand at 38.294 kb on - strand at 38.294 kb on - strand at 38.294 kb on - strand at 38.294 kb on - strand at 38.339 kb on + strand at 38.340 kb on - strand at 38.340 kb on - strand at 38.340 kb on - strand at 38.340 kb on - strand at 38.340 kb on - strand at 38.340 kb on - strand at 38.575 kb on + strand, within EX31_RS14280 at 38.575 kb on + strand, within EX31_RS14280 at 38.575 kb on + strand, within EX31_RS14280 at 38.575 kb on + strand, within EX31_RS14280 at 38.576 kb on - strand, within EX31_RS14280 at 38.576 kb on - strand, within EX31_RS14280 at 38.576 kb on - strand, within EX31_RS14280 at 38.576 kb on - strand, within EX31_RS14280 at 38.576 kb on - strand, within EX31_RS14280 at 38.576 kb on - strand, within EX31_RS14280 at 38.685 kb on + strand, within EX31_RS14280 at 38.685 kb on + strand, within EX31_RS14280 at 38.722 kb on + strand, within EX31_RS14280 at 38.835 kb on + strand, within EX31_RS14280 at 38.836 kb on - strand, within EX31_RS14280 at 38.864 kb on + strand, within EX31_RS14280 at 38.864 kb on + strand, within EX31_RS14280 at 38.940 kb on + strand, within EX31_RS14280 at 38.941 kb on - strand, within EX31_RS14280 at 38.941 kb on - strand, within EX31_RS14280 at 38.941 kb on - strand, within EX31_RS14280 at 39.090 kb on - strand, within EX31_RS14280 at 39.105 kb on + strand, within EX31_RS14280 at 39.105 kb on + strand, within EX31_RS14280 at 39.106 kb on - strand, within EX31_RS14280 at 39.134 kb on + strand, within EX31_RS14280 at 39.224 kb on + strand, within EX31_RS14280 at 39.225 kb on - strand, within EX31_RS14280 at 39.225 kb on - strand, within EX31_RS14280
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 36,239 - EX31_RS14270 0.45 +0.7 36,239 - EX31_RS14270 0.45 +1.2 36,261 + EX31_RS14270 0.47 +0.2 36,262 - EX31_RS14270 0.47 +1.0 36,276 + EX31_RS14270 0.48 -0.3 36,283 - EX31_RS14270 0.48 +0.0 36,283 - EX31_RS14270 0.48 +0.1 36,319 + EX31_RS14270 0.50 +0.3 36,320 - EX31_RS14270 0.50 -1.4 36,320 - EX31_RS14270 0.50 +0.3 36,320 - EX31_RS14270 0.50 +0.6 36,335 + EX31_RS14270 0.51 -0.1 36,336 - EX31_RS14270 0.52 +1.8 36,336 - EX31_RS14270 0.52 -0.1 36,336 - EX31_RS14270 0.52 +0.3 36,361 + EX31_RS14270 0.53 -0.4 36,361 + EX31_RS14270 0.53 +0.5 36,362 - EX31_RS14270 0.53 +0.2 36,362 - EX31_RS14270 0.53 +0.4 36,362 - EX31_RS14270 0.53 -0.1 36,466 + EX31_RS14270 0.60 -0.0 36,466 + EX31_RS14270 0.60 +0.4 36,485 + EX31_RS14270 0.61 +0.7 36,485 + EX31_RS14270 0.61 +1.1 36,485 + EX31_RS14270 0.61 -0.6 36,486 - EX31_RS14270 0.61 +0.8 36,559 + EX31_RS14270 0.66 +0.7 36,595 + EX31_RS14270 0.69 +1.2 36,596 - EX31_RS14270 0.69 +1.2 36,599 + EX31_RS14270 0.69 +0.1 36,599 + EX31_RS14270 0.69 +0.9 36,600 - EX31_RS14270 0.69 -0.4 36,600 - EX31_RS14270 0.69 -0.2 36,644 - EX31_RS14270 0.72 +0.2 36,725 - EX31_RS14270 0.77 +1.5 36,741 - EX31_RS14270 0.78 -0.3 36,741 - EX31_RS14270 0.78 +1.2 36,741 - EX31_RS14270 0.78 -0.3 36,741 - EX31_RS14270 0.78 +0.4 37,005 + +1.4 37,005 + +0.8 37,005 + +1.3 37,005 + -0.1 37,005 + +0.5 37,006 - -0.7 37,006 - -0.1 37,006 - +0.8 37,006 - -0.7 37,006 - +0.3 37,006 - +0.7 37,006 - -0.3 37,015 + -0.1 37,015 + +0.2 37,017 + +0.4 37,017 + +1.0 37,018 - +0.6 37,018 - -0.7 37,072 + +0.7 37,073 - -0.4 37,083 + -1.3 37,083 + +1.9 37,083 + +0.3 37,083 + +0.2 37,083 + +0.2 37,083 + -0.9 37,083 + -2.5 37,083 + -0.0 37,083 + -0.5 37,083 + +0.7 37,084 - -0.2 37,084 - +0.4 37,084 - +0.0 37,084 - +0.1 37,084 - -0.1 37,084 - -0.1 37,084 - +0.9 37,084 - -0.5 37,153 + +0.4 37,205 + +0.1 37,206 - -0.1 37,231 + -1.5 37,231 + +0.5 37,232 - -0.5 37,232 - +2.2 37,232 - +0.6 37,279 - -1.4 37,279 - +0.5 37,343 + EX31_RS14275 0.12 +0.1 37,343 + EX31_RS14275 0.12 +0.4 37,344 - EX31_RS14275 0.12 -0.3 37,352 + EX31_RS14275 0.13 -0.4 37,370 + EX31_RS14275 0.15 -1.5 37,370 + EX31_RS14275 0.15 +0.8 37,371 - EX31_RS14275 0.15 +0.3 37,373 + EX31_RS14275 0.15 -0.2 37,373 + EX31_RS14275 0.15 +1.0 37,373 + EX31_RS14275 0.15 -0.7 37,374 - EX31_RS14275 0.15 +0.5 37,374 - EX31_RS14275 0.15 -0.3 37,374 - EX31_RS14275 0.15 +0.6 37,374 - EX31_RS14275 0.15 +0.5 37,374 - EX31_RS14275 0.15 +0.6 37,374 - EX31_RS14275 0.15 +1.2 37,374 - EX31_RS14275 0.15 +0.6 37,416 + EX31_RS14275 0.20 -0.1 37,416 + EX31_RS14275 0.20 +0.8 37,416 + EX31_RS14275 0.20 -0.5 37,416 + EX31_RS14275 0.20 +0.5 37,416 + EX31_RS14275 0.20 -0.3 37,416 + EX31_RS14275 0.20 -0.5 37,416 + EX31_RS14275 0.20 +0.2 37,416 + EX31_RS14275 0.20 +0.1 37,416 + EX31_RS14275 0.20 +0.2 37,416 + EX31_RS14275 0.20 +1.0 37,417 - EX31_RS14275 0.20 +0.0 37,417 - EX31_RS14275 0.20 +0.6 37,417 - EX31_RS14275 0.20 +1.6 37,417 - EX31_RS14275 0.20 -0.2 37,417 - EX31_RS14275 0.20 +0.6 37,417 - EX31_RS14275 0.20 -0.0 37,417 - EX31_RS14275 0.20 +1.3 37,417 - EX31_RS14275 0.20 -0.5 37,417 - EX31_RS14275 0.20 -0.5 37,417 - EX31_RS14275 0.20 -0.2 37,417 - EX31_RS14275 0.20 +0.2 37,417 - EX31_RS14275 0.20 -0.7 37,418 + EX31_RS14275 0.20 -0.1 37,419 - EX31_RS14275 0.20 +1.5 37,419 - EX31_RS14275 0.20 -0.4 37,475 + EX31_RS14275 0.25 +0.1 37,476 - EX31_RS14275 0.26 +0.6 37,484 + EX31_RS14275 0.26 -0.4 37,485 - EX31_RS14275 0.26 +2.2 37,572 + EX31_RS14275 0.35 +0.8 37,572 + EX31_RS14275 0.35 -0.1 37,572 + EX31_RS14275 0.35 -0.4 37,572 + EX31_RS14275 0.35 +0.2 37,572 + EX31_RS14275 0.35 +0.2 37,572 + EX31_RS14275 0.35 +0.2 37,572 + EX31_RS14275 0.35 +0.9 37,572 + EX31_RS14275 0.35 -0.2 37,572 + EX31_RS14275 0.35 +0.2 37,572 + EX31_RS14275 0.35 -0.5 37,572 + EX31_RS14275 0.35 +0.5 37,572 + EX31_RS14275 0.35 -0.2 37,572 + EX31_RS14275 0.35 +0.7 37,572 + EX31_RS14275 0.35 +0.0 37,572 + EX31_RS14275 0.35 -2.3 37,572 + EX31_RS14275 0.35 -0.4 37,573 - EX31_RS14275 0.35 +0.7 37,573 - EX31_RS14275 0.35 -0.5 37,573 - EX31_RS14275 0.35 -0.6 37,573 - EX31_RS14275 0.35 +0.4 37,573 - EX31_RS14275 0.35 -0.1 37,573 - EX31_RS14275 0.35 -0.2 37,573 - EX31_RS14275 0.35 +0.3 37,573 - EX31_RS14275 0.35 +1.0 37,573 - EX31_RS14275 0.35 +1.4 37,573 - EX31_RS14275 0.35 -0.3 37,573 - EX31_RS14275 0.35 +1.2 37,573 - EX31_RS14275 0.35 +0.5 37,573 - EX31_RS14275 0.35 -0.7 37,573 - EX31_RS14275 0.35 +0.9 37,573 - EX31_RS14275 0.35 +0.1 37,573 - EX31_RS14275 0.35 +0.2 37,573 - EX31_RS14275 0.35 -0.7 37,573 - EX31_RS14275 0.35 +0.4 37,573 - EX31_RS14275 0.35 -0.6 37,573 - EX31_RS14275 0.35 -0.8 37,573 - EX31_RS14275 0.35 +0.4 37,573 - EX31_RS14275 0.35 +0.4 37,617 - EX31_RS14275 0.39 -0.6 37,658 + EX31_RS14275 0.44 +1.4 37,693 + EX31_RS14275 0.47 +0.2 37,693 + EX31_RS14275 0.47 +0.9 37,693 + EX31_RS14275 0.47 -0.4 37,693 + EX31_RS14275 0.47 -1.2 37,693 + EX31_RS14275 0.47 +0.5 37,693 + EX31_RS14275 0.47 +0.8 37,693 + EX31_RS14275 0.47 +0.9 37,693 + EX31_RS14275 0.47 -0.3 37,693 + EX31_RS14275 0.47 -0.0 37,693 + EX31_RS14275 0.47 +0.3 37,694 - EX31_RS14275 0.47 +0.8 37,694 - EX31_RS14275 0.47 +1.2 37,694 - EX31_RS14275 0.47 +0.5 37,694 - EX31_RS14275 0.47 -0.4 37,833 + EX31_RS14275 0.61 -0.7 37,833 + EX31_RS14275 0.61 +0.3 37,833 + EX31_RS14275 0.61 -0.5 37,833 + EX31_RS14275 0.61 +0.1 37,941 - EX31_RS14275 0.72 +0.1 38,030 + EX31_RS14275 0.80 -0.4 38,031 - EX31_RS14275 0.80 -0.9 38,070 - EX31_RS14275 0.84 +0.3 38,072 + EX31_RS14275 0.84 -1.4 38,184 + +0.9 38,184 + +0.5 38,185 - +0.5 38,219 + +0.6 38,220 - -0.5 38,220 - -0.3 38,220 - -0.4 38,220 - +0.2 38,222 + +0.7 38,223 - +0.0 38,293 + -0.4 38,293 + +0.7 38,293 + +0.2 38,293 + -0.0 38,294 - -0.4 38,294 - -1.5 38,294 - +0.7 38,294 - -0.5 38,294 - +0.0 38,339 + -0.8 38,340 - -1.0 38,340 - -1.1 38,340 - -0.8 38,340 - -0.7 38,340 - +0.8 38,340 - -0.1 38,575 + EX31_RS14280 0.24 +0.1 38,575 + EX31_RS14280 0.24 +0.6 38,575 + EX31_RS14280 0.24 -0.1 38,575 + EX31_RS14280 0.24 -0.2 38,576 - EX31_RS14280 0.24 -0.3 38,576 - EX31_RS14280 0.24 -0.2 38,576 - EX31_RS14280 0.24 +1.0 38,576 - EX31_RS14280 0.24 +1.3 38,576 - EX31_RS14280 0.24 +0.6 38,576 - EX31_RS14280 0.24 -1.1 38,685 + EX31_RS14280 0.34 +0.6 38,685 + EX31_RS14280 0.34 -0.9 38,722 + EX31_RS14280 0.37 -0.7 38,835 + EX31_RS14280 0.46 -0.6 38,836 - EX31_RS14280 0.46 -0.8 38,864 + EX31_RS14280 0.48 +0.4 38,864 + EX31_RS14280 0.48 -0.7 38,940 + EX31_RS14280 0.55 -1.6 38,941 - EX31_RS14280 0.55 +0.5 38,941 - EX31_RS14280 0.55 -0.4 38,941 - EX31_RS14280 0.55 +0.9 39,090 - EX31_RS14280 0.67 -0.7 39,105 + EX31_RS14280 0.69 +0.1 39,105 + EX31_RS14280 0.69 -0.8 39,106 - EX31_RS14280 0.69 +0.1 39,134 + EX31_RS14280 0.71 +0.2 39,224 + EX31_RS14280 0.79 +0.1 39,225 - EX31_RS14280 0.79 +0.4 39,225 - EX31_RS14280 0.79 +0.1
Or see this region's nucleotide sequence