Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11060 and EX31_RS11065 are separated by 133 nucleotides EX31_RS11065 and EX31_RS26000 are separated by 14 nucleotides EX31_RS26000 and EX31_RS11070 are separated by 36 nucleotides EX31_RS11070 and EX31_RS11075 are separated by 223 nucleotides
EX31_RS11060: EX31_RS11060 - alkene reductase, at 142,740 to 143,831
_RS11060
EX31_RS11065: EX31_RS11065 - TetR/AcrR family transcriptional regulator, at 143,965 to 144,528
_RS11065
EX31_RS26000: EX31_RS26000 - GntR family transcriptional regulator, at 144,543 to 144,644
_RS26000
EX31_RS11070: EX31_RS11070 - DUF2058 domain-containing protein, at 144,681 to 145,220
_RS11070
EX31_RS11075: EX31_RS11075 - putative motility protein, at 145,444 to 145,632
_RS11075
Position (kb) 143
144
145 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 142.970 kb on + strand, within EX31_RS11060 at 143.007 kb on + strand, within EX31_RS11060 at 143.007 kb on + strand, within EX31_RS11060 at 143.008 kb on - strand, within EX31_RS11060 at 143.008 kb on - strand, within EX31_RS11060 at 143.008 kb on - strand, within EX31_RS11060 at 143.008 kb on - strand, within EX31_RS11060 at 143.051 kb on - strand, within EX31_RS11060 at 143.051 kb on - strand, within EX31_RS11060 at 143.086 kb on + strand, within EX31_RS11060 at 143.087 kb on - strand, within EX31_RS11060 at 143.160 kb on - strand, within EX31_RS11060 at 143.250 kb on - strand, within EX31_RS11060 at 143.496 kb on + strand, within EX31_RS11060 at 143.520 kb on + strand, within EX31_RS11060 at 143.520 kb on + strand, within EX31_RS11060 at 143.520 kb on + strand, within EX31_RS11060 at 143.520 kb on + strand, within EX31_RS11060 at 143.521 kb on - strand, within EX31_RS11060 at 143.521 kb on - strand, within EX31_RS11060 at 143.529 kb on + strand, within EX31_RS11060 at 143.529 kb on + strand, within EX31_RS11060 at 143.530 kb on - strand, within EX31_RS11060 at 143.530 kb on - strand, within EX31_RS11060 at 143.535 kb on + strand, within EX31_RS11060 at 143.536 kb on - strand, within EX31_RS11060 at 143.536 kb on - strand, within EX31_RS11060 at 143.536 kb on - strand, within EX31_RS11060 at 143.542 kb on - strand, within EX31_RS11060 at 143.542 kb on - strand, within EX31_RS11060 at 143.606 kb on + strand, within EX31_RS11060 at 143.606 kb on + strand, within EX31_RS11060 at 143.606 kb on + strand, within EX31_RS11060 at 143.607 kb on - strand, within EX31_RS11060 at 143.607 kb on - strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.608 kb on + strand, within EX31_RS11060 at 143.609 kb on - strand, within EX31_RS11060 at 143.609 kb on - strand, within EX31_RS11060 at 143.609 kb on - strand, within EX31_RS11060 at 143.609 kb on - strand, within EX31_RS11060 at 143.609 kb on - strand, within EX31_RS11060 at 143.621 kb on + strand, within EX31_RS11060 at 143.698 kb on + strand, within EX31_RS11060 at 143.699 kb on - strand, within EX31_RS11060 at 143.699 kb on - strand, within EX31_RS11060 at 143.701 kb on + strand, within EX31_RS11060 at 143.701 kb on + strand, within EX31_RS11060 at 143.702 kb on - strand, within EX31_RS11060 at 143.702 kb on - strand, within EX31_RS11060 at 143.747 kb on + strand at 143.748 kb on - strand at 143.803 kb on + strand at 143.804 kb on - strand at 143.804 kb on - strand at 143.815 kb on - strand at 143.846 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.934 kb on + strand at 143.935 kb on - strand at 143.935 kb on - strand at 144.011 kb on + strand at 144.107 kb on - strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.214 kb on + strand, within EX31_RS11065 at 144.217 kb on + strand, within EX31_RS11065 at 144.217 kb on + strand, within EX31_RS11065 at 144.217 kb on + strand, within EX31_RS11065 at 144.243 kb on + strand, within EX31_RS11065 at 144.350 kb on + strand, within EX31_RS11065 at 144.393 kb on + strand, within EX31_RS11065 at 144.544 kb on + strand at 144.544 kb on + strand at 144.545 kb on - strand at 144.545 kb on - strand at 144.545 kb on - strand at 144.545 kb on - strand at 144.549 kb on - strand at 144.549 kb on - strand at 144.597 kb on + strand, within EX31_RS26000 at 144.598 kb on - strand, within EX31_RS26000 at 144.598 kb on - strand, within EX31_RS26000 at 144.601 kb on + strand, within EX31_RS26000 at 144.601 kb on + strand, within EX31_RS26000 at 144.602 kb on - strand, within EX31_RS26000 at 144.649 kb on + strand at 144.682 kb on + strand at 144.719 kb on - strand at 144.763 kb on - strand, within EX31_RS11070 at 144.763 kb on - strand, within EX31_RS11070 at 144.765 kb on + strand, within EX31_RS11070 at 144.765 kb on + strand, within EX31_RS11070 at 144.765 kb on + strand, within EX31_RS11070 at 144.766 kb on - strand, within EX31_RS11070 at 144.766 kb on - strand, within EX31_RS11070 at 144.766 kb on - strand, within EX31_RS11070 at 144.770 kb on + strand, within EX31_RS11070 at 144.770 kb on + strand, within EX31_RS11070 at 144.771 kb on - strand, within EX31_RS11070 at 144.771 kb on - strand, within EX31_RS11070 at 144.771 kb on - strand, within EX31_RS11070 at 144.771 kb on - strand, within EX31_RS11070 at 144.771 kb on - strand, within EX31_RS11070 at 144.819 kb on - strand, within EX31_RS11070 at 144.826 kb on + strand, within EX31_RS11070 at 144.827 kb on - strand, within EX31_RS11070 at 144.827 kb on - strand, within EX31_RS11070 at 144.882 kb on - strand, within EX31_RS11070 at 144.906 kb on - strand, within EX31_RS11070 at 144.906 kb on - strand, within EX31_RS11070 at 144.932 kb on + strand, within EX31_RS11070 at 144.933 kb on - strand, within EX31_RS11070 at 144.933 kb on - strand, within EX31_RS11070 at 145.014 kb on - strand, within EX31_RS11070 at 145.014 kb on - strand, within EX31_RS11070 at 145.014 kb on - strand, within EX31_RS11070 at 145.016 kb on - strand, within EX31_RS11070 at 145.016 kb on - strand, within EX31_RS11070 at 145.023 kb on - strand, within EX31_RS11070 at 145.023 kb on - strand, within EX31_RS11070 at 145.086 kb on - strand, within EX31_RS11070 at 145.177 kb on - strand at 145.177 kb on - strand at 145.203 kb on + strand at 145.204 kb on - strand at 145.231 kb on - strand at 145.243 kb on + strand at 145.244 kb on - strand at 145.244 kb on - strand at 145.258 kb on + strand at 145.264 kb on + strand at 145.265 kb on - strand at 145.265 kb on - strand at 145.265 kb on - strand at 145.286 kb on + strand at 145.349 kb on + strand at 145.349 kb on + strand at 145.365 kb on + strand at 145.365 kb on + strand at 145.366 kb on - strand at 145.418 kb on + strand at 145.418 kb on + strand at 145.419 kb on - strand at 145.419 kb on - strand at 145.419 kb on - strand at 145.419 kb on - strand at 145.426 kb on - strand at 145.426 kb on - strand at 145.483 kb on - strand, within EX31_RS11075 at 145.485 kb on + strand, within EX31_RS11075 at 145.485 kb on + strand, within EX31_RS11075 at 145.486 kb on - strand, within EX31_RS11075 at 145.486 kb on - strand, within EX31_RS11075 at 145.486 kb on - strand, within EX31_RS11075 at 145.516 kb on - strand, within EX31_RS11075
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 142,970 + EX31_RS11060 0.21 -1.4 143,007 + EX31_RS11060 0.24 -0.4 143,007 + EX31_RS11060 0.24 -0.4 143,008 - EX31_RS11060 0.25 +0.2 143,008 - EX31_RS11060 0.25 +0.6 143,008 - EX31_RS11060 0.25 -0.3 143,008 - EX31_RS11060 0.25 +1.0 143,051 - EX31_RS11060 0.28 +3.2 143,051 - EX31_RS11060 0.28 -0.0 143,086 + EX31_RS11060 0.32 +1.5 143,087 - EX31_RS11060 0.32 -0.7 143,160 - EX31_RS11060 0.38 +0.5 143,250 - EX31_RS11060 0.47 -0.2 143,496 + EX31_RS11060 0.69 +1.2 143,520 + EX31_RS11060 0.71 -1.2 143,520 + EX31_RS11060 0.71 +0.6 143,520 + EX31_RS11060 0.71 +1.0 143,520 + EX31_RS11060 0.71 +0.6 143,521 - EX31_RS11060 0.72 -0.4 143,521 - EX31_RS11060 0.72 +1.0 143,529 + EX31_RS11060 0.72 -0.1 143,529 + EX31_RS11060 0.72 +0.8 143,530 - EX31_RS11060 0.72 -1.0 143,530 - EX31_RS11060 0.72 -0.5 143,535 + EX31_RS11060 0.73 -1.0 143,536 - EX31_RS11060 0.73 -0.7 143,536 - EX31_RS11060 0.73 +0.9 143,536 - EX31_RS11060 0.73 +2.2 143,542 - EX31_RS11060 0.73 +2.2 143,542 - EX31_RS11060 0.73 +0.6 143,606 + EX31_RS11060 0.79 +0.0 143,606 + EX31_RS11060 0.79 +1.2 143,606 + EX31_RS11060 0.79 -2.2 143,607 - EX31_RS11060 0.79 +0.6 143,607 - EX31_RS11060 0.79 +0.5 143,608 + EX31_RS11060 0.79 +0.0 143,608 + EX31_RS11060 0.79 -0.5 143,608 + EX31_RS11060 0.79 +1.2 143,608 + EX31_RS11060 0.79 +1.0 143,608 + EX31_RS11060 0.79 -0.8 143,608 + EX31_RS11060 0.79 -0.3 143,608 + EX31_RS11060 0.79 -0.1 143,608 + EX31_RS11060 0.79 -0.1 143,608 + EX31_RS11060 0.79 -0.6 143,608 + EX31_RS11060 0.79 +2.8 143,608 + EX31_RS11060 0.79 +0.4 143,608 + EX31_RS11060 0.79 -1.4 143,609 - EX31_RS11060 0.80 -0.1 143,609 - EX31_RS11060 0.80 -0.4 143,609 - EX31_RS11060 0.80 -1.0 143,609 - EX31_RS11060 0.80 -0.8 143,609 - EX31_RS11060 0.80 -0.4 143,621 + EX31_RS11060 0.81 +0.6 143,698 + EX31_RS11060 0.88 +1.2 143,699 - EX31_RS11060 0.88 +2.6 143,699 - EX31_RS11060 0.88 +1.6 143,701 + EX31_RS11060 0.88 +3.2 143,701 + EX31_RS11060 0.88 +0.5 143,702 - EX31_RS11060 0.88 -2.3 143,702 - EX31_RS11060 0.88 +0.6 143,747 + -0.2 143,748 - -0.7 143,803 + -2.2 143,804 - +1.8 143,804 - +0.0 143,815 - +1.3 143,846 + -0.2 143,934 + +0.6 143,934 + +0.9 143,934 + -0.4 143,934 + +1.1 143,934 + -0.7 143,934 + +0.6 143,934 + +0.4 143,934 + -0.1 143,934 + -0.2 143,935 - +0.3 143,935 - -0.6 144,011 + -1.7 144,107 - EX31_RS11065 0.25 +0.6 144,214 + EX31_RS11065 0.44 +1.0 144,214 + EX31_RS11065 0.44 -0.3 144,214 + EX31_RS11065 0.44 -1.2 144,214 + EX31_RS11065 0.44 -0.4 144,214 + EX31_RS11065 0.44 -0.8 144,214 + EX31_RS11065 0.44 +0.4 144,214 + EX31_RS11065 0.44 -2.2 144,214 + EX31_RS11065 0.44 -1.2 144,217 + EX31_RS11065 0.45 -1.1 144,217 + EX31_RS11065 0.45 -1.9 144,217 + EX31_RS11065 0.45 -1.4 144,243 + EX31_RS11065 0.49 -1.2 144,350 + EX31_RS11065 0.68 -0.4 144,393 + EX31_RS11065 0.76 +0.1 144,544 + -0.4 144,544 + -0.1 144,545 - -0.6 144,545 - +0.6 144,545 - -0.6 144,545 - +1.8 144,549 - +0.8 144,549 - -1.1 144,597 + EX31_RS26000 0.53 +0.0 144,598 - EX31_RS26000 0.54 +0.3 144,598 - EX31_RS26000 0.54 +0.6 144,601 + EX31_RS26000 0.57 +1.1 144,601 + EX31_RS26000 0.57 +0.6 144,602 - EX31_RS26000 0.58 +1.6 144,649 + +0.1 144,682 + +2.2 144,719 - +0.2 144,763 - EX31_RS11070 0.15 +1.2 144,763 - EX31_RS11070 0.15 +1.6 144,765 + EX31_RS11070 0.16 -2.2 144,765 + EX31_RS11070 0.16 -1.4 144,765 + EX31_RS11070 0.16 +0.6 144,766 - EX31_RS11070 0.16 +0.4 144,766 - EX31_RS11070 0.16 +0.4 144,766 - EX31_RS11070 0.16 -2.0 144,770 + EX31_RS11070 0.16 +1.6 144,770 + EX31_RS11070 0.16 -1.4 144,771 - EX31_RS11070 0.17 -2.0 144,771 - EX31_RS11070 0.17 -1.9 144,771 - EX31_RS11070 0.17 -0.1 144,771 - EX31_RS11070 0.17 +0.6 144,771 - EX31_RS11070 0.17 -1.3 144,819 - EX31_RS11070 0.26 -0.7 144,826 + EX31_RS11070 0.27 -0.9 144,827 - EX31_RS11070 0.27 +0.0 144,827 - EX31_RS11070 0.27 -0.4 144,882 - EX31_RS11070 0.37 +0.8 144,906 - EX31_RS11070 0.42 +0.7 144,906 - EX31_RS11070 0.42 -0.4 144,932 + EX31_RS11070 0.46 +1.4 144,933 - EX31_RS11070 0.47 -1.0 144,933 - EX31_RS11070 0.47 +0.2 145,014 - EX31_RS11070 0.62 -1.2 145,014 - EX31_RS11070 0.62 -0.2 145,014 - EX31_RS11070 0.62 +0.3 145,016 - EX31_RS11070 0.62 +1.0 145,016 - EX31_RS11070 0.62 +0.4 145,023 - EX31_RS11070 0.63 +0.6 145,023 - EX31_RS11070 0.63 -1.0 145,086 - EX31_RS11070 0.75 +0.4 145,177 - -0.4 145,177 - +0.6 145,203 + -0.1 145,204 - +1.5 145,231 - -0.4 145,243 + -1.4 145,244 - +0.4 145,244 - -0.4 145,258 + +0.6 145,264 + -1.4 145,265 - +0.6 145,265 - +2.2 145,265 - -1.0 145,286 + -1.2 145,349 + +1.2 145,349 + -2.2 145,365 + -0.5 145,365 + -0.7 145,366 - +0.0 145,418 + +0.1 145,418 + -0.1 145,419 - +0.6 145,419 - +0.4 145,419 - -0.4 145,419 - -0.8 145,426 - +0.0 145,426 - +1.3 145,483 - EX31_RS11075 0.21 +1.6 145,485 + EX31_RS11075 0.22 +0.3 145,485 + EX31_RS11075 0.22 +0.6 145,486 - EX31_RS11075 0.22 -0.8 145,486 - EX31_RS11075 0.22 +1.9 145,486 - EX31_RS11075 0.22 -0.1 145,516 - EX31_RS11075 0.38 +0.6
Or see this region's nucleotide sequence