Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02865 and CA265_RS02870 are separated by 82 nucleotides CA265_RS02870 and CA265_RS02875 are separated by 109 nucleotides CA265_RS02875 and CA265_RS02880 are separated by 9 nucleotides CA265_RS02880 and CA265_RS02885 are separated by 375 nucleotides
CA265_RS02865: CA265_RS02865 - elongation factor Tu, at 714,719 to 715,906
_RS02865
CA265_RS02870: CA265_RS02870 - tRNA-Trp, at 715,989 to 716,059
_RS02870
CA265_RS02875: CA265_RS02875 - preprotein translocase subunit SecE, at 716,169 to 716,366
_RS02875
CA265_RS02880: CA265_RS02880 - transcription termination/antitermination factor NusG, at 716,376 to 716,915
_RS02880
CA265_RS02885: CA265_RS02885 - hypothetical protein, at 717,291 to 717,800
_RS02885
Position (kb)
716
717 Strain fitness (log2 ratio)
-2
-1
0
1 at 717.027 kb on - strand at 717.053 kb on - strand at 717.056 kb on + strand at 717.056 kb on + strand at 717.056 kb on + strand at 717.057 kb on - strand at 717.074 kb on - strand at 717.083 kb on + strand at 717.124 kb on + strand at 717.124 kb on + strand at 717.136 kb on - strand at 717.136 kb on - strand at 717.136 kb on - strand at 717.274 kb on - strand at 717.274 kb on - strand at 717.274 kb on - strand at 717.346 kb on + strand, within CA265_RS02885 at 717.346 kb on + strand, within CA265_RS02885 at 717.346 kb on + strand, within CA265_RS02885 at 717.349 kb on + strand, within CA265_RS02885 at 717.349 kb on + strand, within CA265_RS02885
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 717,027 - -0.2 717,053 - -2.0 717,056 + -1.6 717,056 + +0.1 717,056 + -0.2 717,057 - -1.6 717,074 - -0.4 717,083 + -0.3 717,124 + -0.0 717,124 + +0.1 717,136 - -2.0 717,136 - -0.1 717,136 - -0.5 717,274 - +0.5 717,274 - -1.2 717,274 - +0.9 717,346 + CA265_RS02885 0.11 +0.3 717,346 + CA265_RS02885 0.11 +0.6 717,346 + CA265_RS02885 0.11 -2.1 717,349 + CA265_RS02885 0.11 -0.1 717,349 + CA265_RS02885 0.11 +0.3
Or see this region's nucleotide sequence