Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35995 and BPHYT_RS36000 are separated by 242 nucleotides BPHYT_RS36000 and BPHYT_RS36005 are separated by 151 nucleotides BPHYT_RS36005 and BPHYT_RS36010 are separated by 63 nucleotides BPHYT_RS36010 and BPHYT_RS36015 are separated by 58 nucleotides BPHYT_RS36015 and BPHYT_RS36020 are separated by 181 nucleotides
BPHYT_RS35995: BPHYT_RS35995 - hypothetical protein, at 32,185 to 32,418
_RS35995
BPHYT_RS36000: BPHYT_RS36000 - hypothetical protein, at 32,661 to 32,903
_RS36000
BPHYT_RS36005: BPHYT_RS36005 - hypothetical protein, at 33,055 to 33,375
_RS36005
BPHYT_RS36010: BPHYT_RS36010 - hypothetical protein, at 33,439 to 33,915
_RS36010
BPHYT_RS36015: BPHYT_RS36015 - hypothetical protein, at 33,974 to 34,153
_RS36015
BPHYT_RS36020: BPHYT_RS36020 - hypothetical protein, at 34,335 to 34,571
_RS36020
Position (kb) 33
34 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 32.125 kb on + strand at 32.172 kb on - strand at 32.286 kb on + strand, within BPHYT_RS35995 at 32.321 kb on + strand, within BPHYT_RS35995 at 32.330 kb on + strand, within BPHYT_RS35995 at 32.330 kb on + strand, within BPHYT_RS35995 at 32.331 kb on - strand, within BPHYT_RS35995 at 32.331 kb on - strand, within BPHYT_RS35995 at 32.380 kb on + strand, within BPHYT_RS35995 at 32.487 kb on + strand at 32.488 kb on - strand at 32.501 kb on - strand at 32.814 kb on - strand, within BPHYT_RS36000 at 32.829 kb on - strand, within BPHYT_RS36000 at 32.829 kb on - strand, within BPHYT_RS36000 at 32.933 kb on - strand at 32.949 kb on + strand at 33.030 kb on + strand at 33.033 kb on + strand at 33.033 kb on + strand at 33.109 kb on + strand, within BPHYT_RS36005 at 33.110 kb on - strand, within BPHYT_RS36005 at 33.110 kb on - strand, within BPHYT_RS36005 at 33.239 kb on - strand at 33.239 kb on - strand, within BPHYT_RS36005 at 33.329 kb on - strand, within BPHYT_RS36005 at 33.358 kb on + strand at 33.359 kb on - strand at 33.359 kb on - strand at 33.560 kb on + strand, within BPHYT_RS36010 at 33.774 kb on + strand, within BPHYT_RS36010 at 33.775 kb on - strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.788 kb on - strand, within BPHYT_RS36010 at 33.847 kb on + strand, within BPHYT_RS36010 at 33.902 kb on + strand at 33.924 kb on - strand at 33.924 kb on - strand at 34.153 kb on + strand at 34.154 kb on - strand at 34.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 32,125 + -1.2 32,172 - -2.2 32,286 + BPHYT_RS35995 0.43 +0.2 32,321 + BPHYT_RS35995 0.58 -0.2 32,330 + BPHYT_RS35995 0.62 +0.9 32,330 + BPHYT_RS35995 0.62 -0.8 32,331 - BPHYT_RS35995 0.62 -1.3 32,331 - BPHYT_RS35995 0.62 -3.1 32,380 + BPHYT_RS35995 0.83 +0.6 32,487 + -1.6 32,488 - -2.4 32,501 - -0.4 32,814 - BPHYT_RS36000 0.63 -2.4 32,829 - BPHYT_RS36000 0.69 -0.6 32,829 - BPHYT_RS36000 0.69 -1.9 32,933 - -1.4 32,949 + -0.0 33,030 + -0.7 33,033 + +0.1 33,033 + +0.2 33,109 + BPHYT_RS36005 0.17 +0.1 33,110 - BPHYT_RS36005 0.17 -1.3 33,110 - BPHYT_RS36005 0.17 -1.9 33,239 - -1.6 33,239 - BPHYT_RS36005 0.57 -3.8 33,329 - BPHYT_RS36005 0.85 -1.5 33,358 + -2.1 33,359 - -1.9 33,359 - -3.0 33,560 + BPHYT_RS36010 0.25 -1.0 33,774 + BPHYT_RS36010 0.70 +4.2 33,775 - BPHYT_RS36010 0.70 +2.7 33,787 + BPHYT_RS36010 0.73 +2.2 33,787 + BPHYT_RS36010 0.73 +0.8 33,787 + BPHYT_RS36010 0.73 -0.3 33,788 - BPHYT_RS36010 0.73 -2.3 33,847 + BPHYT_RS36010 0.86 -0.4 33,902 + +0.2 33,924 - -0.0 33,924 - -2.1 34,153 + +0.1 34,154 - -2.3 34,273 - -2.7
Or see this region's nucleotide sequence