Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35645

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35640 and BPHYT_RS35645 are separated by 620 nucleotidesBPHYT_RS35645 and BPHYT_RS35650 are separated by 120 nucleotides BPHYT_RS35640: BPHYT_RS35640 - hypothetical protein, at 3,588,771 to 3,589,217 _RS35640 BPHYT_RS35645: BPHYT_RS35645 - porin, at 3,589,838 to 3,590,998 _RS35645 BPHYT_RS35650: BPHYT_RS35650 - DSBA oxidoreductase, at 3,591,119 to 3,591,784 _RS35650 Position (kb) 3589 3590 3591Strain fitness (log2 ratio) -2 -1 0 1 2at 3588.923 kb on - strand, within BPHYT_RS35640at 3588.923 kb on - strand, within BPHYT_RS35640at 3588.983 kb on - strand, within BPHYT_RS35640at 3589.021 kb on - strand, within BPHYT_RS35640at 3589.081 kb on - strand, within BPHYT_RS35640at 3589.103 kb on - strand, within BPHYT_RS35640at 3589.103 kb on - strand, within BPHYT_RS35640at 3589.206 kb on - strandat 3589.435 kb on + strandat 3589.653 kb on + strandat 3589.717 kb on + strandat 3589.945 kb on + strandat 3589.946 kb on - strandat 3590.539 kb on + strand, within BPHYT_RS35645at 3590.561 kb on + strand, within BPHYT_RS35645at 3590.562 kb on - strand, within BPHYT_RS35645at 3590.652 kb on - strand, within BPHYT_RS35645at 3590.765 kb on + strand, within BPHYT_RS35645at 3590.787 kb on - strand, within BPHYT_RS35645at 3590.788 kb on + strand, within BPHYT_RS35645at 3590.996 kb on + strandat 3591.076 kb on + strandat 3591.121 kb on - strandat 3591.265 kb on + strand, within BPHYT_RS35650at 3591.297 kb on + strand, within BPHYT_RS35650at 3591.298 kb on - strand, within BPHYT_RS35650at 3591.492 kb on + strand, within BPHYT_RS35650at 3591.588 kb on + strand, within BPHYT_RS35650at 3591.953 kb on + strandat 3591.954 kb on - strandat 3591.963 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,588,923 - BPHYT_RS35640 0.34 +0.6
3,588,923 - BPHYT_RS35640 0.34 -0.3
3,588,983 - BPHYT_RS35640 0.47 +0.0
3,589,021 - BPHYT_RS35640 0.56 -0.2
3,589,081 - BPHYT_RS35640 0.69 +0.8
3,589,103 - BPHYT_RS35640 0.74 +1.0
3,589,103 - BPHYT_RS35640 0.74 -0.7
3,589,206 - -0.3
3,589,435 + +0.1
3,589,653 + -0.2
3,589,717 + -2.0
3,589,945 + -0.9
3,589,946 - -0.3
3,590,539 + BPHYT_RS35645 0.60 +0.4
3,590,561 + BPHYT_RS35645 0.62 -0.0
3,590,562 - BPHYT_RS35645 0.62 +0.3
3,590,652 - BPHYT_RS35645 0.70 -0.2
3,590,765 + BPHYT_RS35645 0.80 +0.3
3,590,787 - BPHYT_RS35645 0.82 +1.9
3,590,788 + BPHYT_RS35645 0.82 +1.3
3,590,996 + +0.3
3,591,076 + +0.2
3,591,121 - -0.3
3,591,265 + BPHYT_RS35650 0.22 -0.6
3,591,297 + BPHYT_RS35650 0.27 -1.3
3,591,298 - BPHYT_RS35650 0.27 -0.8
3,591,492 + BPHYT_RS35650 0.56 -1.1
3,591,588 + BPHYT_RS35650 0.70 -0.4
3,591,953 + +1.0
3,591,954 - +0.7
3,591,963 - +0.2

Or see this region's nucleotide sequence