Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34405 and BPHYT_RS34410 are separated by 435 nucleotides BPHYT_RS34410 and BPHYT_RS34415 overlap by 41 nucleotides BPHYT_RS34415 and BPHYT_RS34420 are separated by 158 nucleotides
BPHYT_RS34405: BPHYT_RS34405 - hypothetical protein, at 3,325,175 to 3,327,358
_RS34405
BPHYT_RS34410: BPHYT_RS34410 - hypothetical protein, at 3,327,794 to 3,328,060
_RS34410
BPHYT_RS34415: BPHYT_RS34415 - multidrug DMT transporter permease, at 3,328,020 to 3,328,919
_RS34415
BPHYT_RS34420: BPHYT_RS34420 - AraC family transcriptional regulator, at 3,329,078 to 3,329,995
_RS34420
Position (kb)
3328
3329 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3327.039 kb on + strand, within BPHYT_RS34405 at 3327.040 kb on - strand, within BPHYT_RS34405 at 3327.040 kb on - strand, within BPHYT_RS34405 at 3327.171 kb on + strand at 3327.270 kb on + strand at 3327.271 kb on - strand at 3327.307 kb on + strand at 3327.365 kb on - strand at 3327.379 kb on + strand at 3327.379 kb on + strand at 3327.566 kb on + strand at 3327.574 kb on + strand at 3327.754 kb on + strand at 3327.878 kb on + strand, within BPHYT_RS34410 at 3328.141 kb on - strand, within BPHYT_RS34415 at 3328.229 kb on - strand, within BPHYT_RS34415 at 3328.267 kb on + strand, within BPHYT_RS34415 at 3328.286 kb on - strand, within BPHYT_RS34415 at 3328.373 kb on + strand, within BPHYT_RS34415 at 3328.678 kb on + strand, within BPHYT_RS34415 at 3328.679 kb on - strand, within BPHYT_RS34415 at 3328.903 kb on + strand at 3328.903 kb on + strand at 3329.035 kb on - strand at 3329.064 kb on - strand at 3329.285 kb on + strand, within BPHYT_RS34420 at 3329.285 kb on + strand, within BPHYT_RS34420 at 3329.333 kb on + strand, within BPHYT_RS34420 at 3329.420 kb on + strand, within BPHYT_RS34420 at 3329.533 kb on - strand, within BPHYT_RS34420 at 3329.533 kb on - strand, within BPHYT_RS34420 at 3329.702 kb on + strand, within BPHYT_RS34420 at 3329.702 kb on + strand, within BPHYT_RS34420
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,327,039 + BPHYT_RS34405 0.85 -0.1 3,327,040 - BPHYT_RS34405 0.85 -0.7 3,327,040 - BPHYT_RS34405 0.85 +0.4 3,327,171 + +0.1 3,327,270 + +4.0 3,327,271 - -0.7 3,327,307 + -0.5 3,327,365 - -0.1 3,327,379 + -0.3 3,327,379 + -0.8 3,327,566 + +0.3 3,327,574 + -1.0 3,327,754 + -1.7 3,327,878 + BPHYT_RS34410 0.31 +0.6 3,328,141 - BPHYT_RS34415 0.13 +1.3 3,328,229 - BPHYT_RS34415 0.23 -0.2 3,328,267 + BPHYT_RS34415 0.27 -0.7 3,328,286 - BPHYT_RS34415 0.30 -0.3 3,328,373 + BPHYT_RS34415 0.39 +0.2 3,328,678 + BPHYT_RS34415 0.73 -0.5 3,328,679 - BPHYT_RS34415 0.73 -0.7 3,328,903 + -0.2 3,328,903 + -0.1 3,329,035 - +0.7 3,329,064 - -0.1 3,329,285 + BPHYT_RS34420 0.23 +2.3 3,329,285 + BPHYT_RS34420 0.23 +0.3 3,329,333 + BPHYT_RS34420 0.28 +0.7 3,329,420 + BPHYT_RS34420 0.37 +0.1 3,329,533 - BPHYT_RS34420 0.50 -1.8 3,329,533 - BPHYT_RS34420 0.50 -0.2 3,329,702 + BPHYT_RS34420 0.68 +1.1 3,329,702 + BPHYT_RS34420 0.68 -1.7
Or see this region's nucleotide sequence