Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30800

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30790 and BPHYT_RS30795 are separated by 177 nucleotidesBPHYT_RS30795 and BPHYT_RS30800 overlap by 4 nucleotidesBPHYT_RS30800 and BPHYT_RS30805 are separated by 145 nucleotides BPHYT_RS30790: BPHYT_RS30790 - porin, at 2,463,352 to 2,464,521 _RS30790 BPHYT_RS30795: BPHYT_RS30795 - short-chain dehydrogenase, at 2,464,699 to 2,465,451 _RS30795 BPHYT_RS30800: BPHYT_RS30800 - MFS transporter, at 2,465,448 to 2,466,731 _RS30800 BPHYT_RS30805: BPHYT_RS30805 - MFS transporter, at 2,466,877 to 2,468,160 _RS30805 Position (kb) 2465 2466 2467Strain fitness (log2 ratio) -1 0 1at 2464.606 kb on + strandat 2465.067 kb on - strand, within BPHYT_RS30795at 2465.093 kb on + strand, within BPHYT_RS30795at 2465.478 kb on + strandat 2465.881 kb on + strand, within BPHYT_RS30800at 2466.872 kb on + strandat 2466.873 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,464,606 + -0.1
2,465,067 - BPHYT_RS30795 0.49 -1.1
2,465,093 + BPHYT_RS30795 0.52 -1.2
2,465,478 + +0.7
2,465,881 + BPHYT_RS30800 0.34 -0.6
2,466,872 + -0.8
2,466,873 - -1.3

Or see this region's nucleotide sequence