Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25475 and BPHYT_RS25480 are separated by 173 nucleotides BPHYT_RS25480 and BPHYT_RS25485 are separated by 91 nucleotides BPHYT_RS25485 and BPHYT_RS25490 overlap by 11 nucleotides BPHYT_RS25490 and BPHYT_RS25495 are separated by 32 nucleotides
BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064
_RS25475
BPHYT_RS25480: BPHYT_RS25480 - hypothetical protein, at 1,294,238 to 1,294,879
_RS25480
BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750
_RS25485
BPHYT_RS25490: BPHYT_RS25490 - hypothetical protein, at 1,295,740 to 1,296,663
_RS25490
BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007
_RS25495
Position (kb) 1294
1295
1296 Strain fitness (log2 ratio) -1
0
1 at 1294.151 kb on + strand at 1294.353 kb on + strand, within BPHYT_RS25480 at 1294.355 kb on + strand, within BPHYT_RS25480 at 1294.508 kb on - strand, within BPHYT_RS25480 at 1294.511 kb on + strand, within BPHYT_RS25480 at 1294.512 kb on - strand, within BPHYT_RS25480 at 1295.848 kb on - strand, within BPHYT_RS25490 at 1295.982 kb on + strand, within BPHYT_RS25490 at 1296.035 kb on + strand, within BPHYT_RS25490 at 1296.084 kb on + strand, within BPHYT_RS25490 at 1296.495 kb on + strand, within BPHYT_RS25490 at 1296.498 kb on - strand, within BPHYT_RS25490 at 1296.654 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,294,151 + -0.1 1,294,353 + BPHYT_RS25480 0.18 -0.6 1,294,355 + BPHYT_RS25480 0.18 -0.3 1,294,508 - BPHYT_RS25480 0.42 -0.1 1,294,511 + BPHYT_RS25480 0.43 -0.2 1,294,512 - BPHYT_RS25480 0.43 -0.0 1,295,848 - BPHYT_RS25490 0.12 -0.2 1,295,982 + BPHYT_RS25490 0.26 -0.0 1,296,035 + BPHYT_RS25490 0.32 -0.9 1,296,084 + BPHYT_RS25490 0.37 -0.6 1,296,495 + BPHYT_RS25490 0.82 +0.6 1,296,498 - BPHYT_RS25490 0.82 +1.0 1,296,654 + +0.1
Or see this region's nucleotide sequence