Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25460 and BPHYT_RS25465 are separated by 385 nucleotides BPHYT_RS25465 and BPHYT_RS25470 are separated by 26 nucleotides BPHYT_RS25470 and BPHYT_RS25475 are separated by 115 nucleotides
BPHYT_RS25460: BPHYT_RS25460 - LysR family transcriptional regulator, at 1,290,101 to 1,290,997
_RS25460
BPHYT_RS25465: BPHYT_RS25465 - 1,2-oxophytodienoate reductase, at 1,291,383 to 1,292,471
_RS25465
BPHYT_RS25470: BPHYT_RS25470 - alkylhydroperoxidase, at 1,292,498 to 1,293,034
_RS25470
BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064
_RS25475
Position (kb) 1291
1292
1293 Strain fitness (log2 ratio) -1
0
1
2 at 1291.300 kb on + strand at 1291.409 kb on - strand at 1291.416 kb on - strand at 1291.526 kb on - strand, within BPHYT_RS25465 at 1291.821 kb on + strand, within BPHYT_RS25465 at 1292.059 kb on - strand, within BPHYT_RS25465 at 1292.208 kb on + strand, within BPHYT_RS25465 at 1292.342 kb on + strand, within BPHYT_RS25465 at 1292.476 kb on - strand at 1292.710 kb on + strand, within BPHYT_RS25470 at 1292.711 kb on - strand, within BPHYT_RS25470 at 1293.099 kb on - strand at 1293.099 kb on - strand at 1293.139 kb on + strand at 1293.293 kb on - strand, within BPHYT_RS25475
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,291,300 + -0.7 1,291,409 - +0.0 1,291,416 - -0.4 1,291,526 - BPHYT_RS25465 0.13 +0.2 1,291,821 + BPHYT_RS25465 0.40 -1.5 1,292,059 - BPHYT_RS25465 0.62 +0.4 1,292,208 + BPHYT_RS25465 0.76 +0.5 1,292,342 + BPHYT_RS25465 0.88 -0.0 1,292,476 - +0.3 1,292,710 + BPHYT_RS25470 0.39 +1.4 1,292,711 - BPHYT_RS25470 0.40 +2.3 1,293,099 - +0.5 1,293,099 - -0.3 1,293,139 + +0.2 1,293,293 - BPHYT_RS25475 0.16 +0.7
Or see this region's nucleotide sequence