Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21045

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21040 and BPHYT_RS21045 are separated by 178 nucleotidesBPHYT_RS21045 and BPHYT_RS21050 are separated by 523 nucleotides BPHYT_RS21040: BPHYT_RS21040 - ABC transporter permease, at 280,614 to 281,540 _RS21040 BPHYT_RS21045: BPHYT_RS21045 - bis(5'-nucleosyl)-tetraphosphatase, at 281,719 to 282,801 _RS21045 BPHYT_RS21050: BPHYT_RS21050 - aldehyde-activating protein, at 283,325 to 283,900 _RS21050 Position (kb) 281 282 283Strain fitness (log2 ratio) -3 -2 -1 0 1at 281.374 kb on + strand, within BPHYT_RS21040at 281.831 kb on - strand, within BPHYT_RS21045at 281.977 kb on - strand, within BPHYT_RS21045at 282.139 kb on + strand, within BPHYT_RS21045at 282.618 kb on - strand, within BPHYT_RS21045at 282.638 kb on + strand, within BPHYT_RS21045at 282.683 kb on - strand, within BPHYT_RS21045at 282.818 kb on - strandat 282.921 kb on + strandat 282.970 kb on + strandat 282.979 kb on + strandat 282.979 kb on + strandat 282.979 kb on + strandat 283.080 kb on + strandat 283.297 kb on + strandat 283.301 kb on - strandat 283.304 kb on - strandat 283.398 kb on + strand, within BPHYT_RS21050at 283.398 kb on + strand, within BPHYT_RS21050at 283.474 kb on + strand, within BPHYT_RS21050at 283.632 kb on - strand, within BPHYT_RS21050

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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281,374 + BPHYT_RS21040 0.82 +0.6
281,831 - BPHYT_RS21045 0.10 -0.8
281,977 - BPHYT_RS21045 0.24 -1.2
282,139 + BPHYT_RS21045 0.39 -3.6
282,618 - BPHYT_RS21045 0.83 -1.9
282,638 + BPHYT_RS21045 0.85 +1.4
282,683 - BPHYT_RS21045 0.89 -0.5
282,818 - -0.0
282,921 + -0.5
282,970 + -0.6
282,979 + -0.5
282,979 + -0.2
282,979 + +0.6
283,080 + +1.0
283,297 + -1.0
283,301 - -1.6
283,304 - -0.5
283,398 + BPHYT_RS21050 0.13 +0.4
283,398 + BPHYT_RS21050 0.13 +0.5
283,474 + BPHYT_RS21050 0.26 +1.1
283,632 - BPHYT_RS21050 0.53 +0.6

Or see this region's nucleotide sequence