Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20150

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20140 and BPHYT_RS20145 overlap by 4 nucleotidesBPHYT_RS20145 and BPHYT_RS20150 are separated by 77 nucleotidesBPHYT_RS20150 and BPHYT_RS20155 overlap by 4 nucleotides BPHYT_RS20140: BPHYT_RS20140 - hypothetical protein, at 61,098 to 62,264 _RS20140 BPHYT_RS20145: BPHYT_RS20145 - hypothetical protein, at 62,261 to 62,977 _RS20145 BPHYT_RS20150: BPHYT_RS20150 - glycosyl transferase family 1, at 63,055 to 64,146 _RS20150 BPHYT_RS20155: BPHYT_RS20155 - hypothetical protein, at 64,143 to 65,402 _RS20155 Position (kb) 63 64 65Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 62.160 kb on + strandat 62.161 kb on - strandat 62.592 kb on + strand, within BPHYT_RS20145at 63.093 kb on - strandat 63.376 kb on + strand, within BPHYT_RS20150at 63.756 kb on + strand, within BPHYT_RS20150at 63.756 kb on + strand, within BPHYT_RS20150at 64.133 kb on - strandat 64.381 kb on - strand, within BPHYT_RS20155at 64.742 kb on - strand, within BPHYT_RS20155

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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62,160 + +2.4
62,161 - +0.4
62,592 + BPHYT_RS20145 0.46 +0.4
63,093 - -0.4
63,376 + BPHYT_RS20150 0.29 +0.4
63,756 + BPHYT_RS20150 0.64 -0.6
63,756 + BPHYT_RS20150 0.64 -0.2
64,133 - +0.6
64,381 - BPHYT_RS20155 0.19 -1.5
64,742 - BPHYT_RS20155 0.48 -3.5

Or see this region's nucleotide sequence