Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18870 and BPHYT_RS18875 are separated by 12 nucleotides BPHYT_RS18875 and BPHYT_RS18880 are separated by 20 nucleotides BPHYT_RS18880 and BPHYT_RS18885 are separated by 43 nucleotides BPHYT_RS18885 and BPHYT_RS18890 are separated by 10 nucleotides
BPHYT_RS18870: BPHYT_RS18870 - flagellar basal body rod protein FlgG, at 4,240,929 to 4,241,717
_RS18870
BPHYT_RS18875: BPHYT_RS18875 - flagellar basal body rod protein FlgF, at 4,241,730 to 4,242,488
_RS18875
BPHYT_RS18880: BPHYT_RS18880 - flagellar hook protein FlgE, at 4,242,509 to 4,244,020
_RS18880
BPHYT_RS18885: BPHYT_RS18885 - flagellar basal body rod modification protein FlgD, at 4,244,064 to 4,244,786
_RS18885
BPHYT_RS18890: BPHYT_RS18890 - flagellar basal body rod protein FlgC, at 4,244,797 to 4,245,222
_RS18890
Position (kb) 4242
4243
4244
4245 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 4241.582 kb on - strand, within BPHYT_RS18870 at 4241.701 kb on + strand at 4241.701 kb on + strand at 4241.701 kb on + strand at 4241.702 kb on - strand at 4241.735 kb on + strand at 4241.736 kb on - strand at 4241.736 kb on - strand at 4242.175 kb on + strand, within BPHYT_RS18875 at 4242.176 kb on - strand, within BPHYT_RS18875 at 4242.274 kb on + strand, within BPHYT_RS18875 at 4242.274 kb on + strand, within BPHYT_RS18875 at 4242.275 kb on - strand, within BPHYT_RS18875 at 4242.511 kb on - strand at 4242.511 kb on - strand at 4242.574 kb on - strand at 4242.574 kb on - strand at 4242.574 kb on - strand at 4242.909 kb on + strand, within BPHYT_RS18880 at 4242.909 kb on + strand, within BPHYT_RS18880 at 4242.909 kb on + strand, within BPHYT_RS18880 at 4242.910 kb on - strand, within BPHYT_RS18880 at 4242.987 kb on + strand, within BPHYT_RS18880 at 4242.987 kb on + strand, within BPHYT_RS18880 at 4242.987 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.197 kb on + strand, within BPHYT_RS18880 at 4243.198 kb on - strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.204 kb on - strand, within BPHYT_RS18880 at 4243.333 kb on - strand, within BPHYT_RS18880 at 4243.410 kb on + strand, within BPHYT_RS18880 at 4243.410 kb on + strand, within BPHYT_RS18880 at 4243.411 kb on - strand, within BPHYT_RS18880 at 4243.411 kb on - strand, within BPHYT_RS18880 at 4243.723 kb on - strand, within BPHYT_RS18880 at 4243.739 kb on - strand, within BPHYT_RS18880 at 4243.869 kb on - strand at 4243.893 kb on + strand at 4243.894 kb on - strand at 4243.894 kb on - strand at 4243.936 kb on + strand at 4244.032 kb on + strand at 4244.033 kb on - strand at 4244.130 kb on - strand at 4244.227 kb on + strand, within BPHYT_RS18885 at 4244.228 kb on - strand, within BPHYT_RS18885 at 4244.228 kb on - strand, within BPHYT_RS18885 at 4244.547 kb on - strand, within BPHYT_RS18885 at 4244.547 kb on - strand, within BPHYT_RS18885 at 4244.726 kb on + strand at 4244.727 kb on - strand at 4244.933 kb on + strand, within BPHYT_RS18890 at 4244.933 kb on + strand, within BPHYT_RS18890 at 4244.934 kb on - strand, within BPHYT_RS18890
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,241,582 - BPHYT_RS18870 0.83 -4.9 4,241,701 + -6.1 4,241,701 + -3.0 4,241,701 + -5.3 4,241,702 - -0.2 4,241,735 + -3.9 4,241,736 - -1.4 4,241,736 - -2.7 4,242,175 + BPHYT_RS18875 0.59 -6.2 4,242,176 - BPHYT_RS18875 0.59 -5.6 4,242,274 + BPHYT_RS18875 0.72 -2.2 4,242,274 + BPHYT_RS18875 0.72 -3.5 4,242,275 - BPHYT_RS18875 0.72 -5.0 4,242,511 - -2.4 4,242,511 - -1.4 4,242,574 - -4.2 4,242,574 - -5.4 4,242,574 - -4.3 4,242,909 + BPHYT_RS18880 0.26 -3.3 4,242,909 + BPHYT_RS18880 0.26 -5.0 4,242,909 + BPHYT_RS18880 0.26 -4.7 4,242,910 - BPHYT_RS18880 0.27 -4.9 4,242,987 + BPHYT_RS18880 0.32 -4.7 4,242,987 + BPHYT_RS18880 0.32 -3.6 4,242,987 + BPHYT_RS18880 0.32 -2.7 4,243,140 + BPHYT_RS18880 0.42 -3.8 4,243,140 + BPHYT_RS18880 0.42 -3.9 4,243,140 + BPHYT_RS18880 0.42 -3.9 4,243,176 + BPHYT_RS18880 0.44 -5.3 4,243,176 + BPHYT_RS18880 0.44 -4.7 4,243,176 + BPHYT_RS18880 0.44 -4.6 4,243,197 + BPHYT_RS18880 0.46 -2.3 4,243,198 - BPHYT_RS18880 0.46 -2.7 4,243,203 + BPHYT_RS18880 0.46 -2.6 4,243,203 + BPHYT_RS18880 0.46 -2.4 4,243,203 + BPHYT_RS18880 0.46 -1.2 4,243,204 - BPHYT_RS18880 0.46 -5.6 4,243,333 - BPHYT_RS18880 0.54 -2.6 4,243,410 + BPHYT_RS18880 0.60 -2.2 4,243,410 + BPHYT_RS18880 0.60 -2.3 4,243,411 - BPHYT_RS18880 0.60 -5.7 4,243,411 - BPHYT_RS18880 0.60 -2.6 4,243,723 - BPHYT_RS18880 0.80 -3.4 4,243,739 - BPHYT_RS18880 0.81 -4.6 4,243,869 - -3.3 4,243,893 + -4.2 4,243,894 - -4.5 4,243,894 - -4.8 4,243,936 + -4.3 4,244,032 + -2.6 4,244,033 - -2.1 4,244,130 - -3.2 4,244,227 + BPHYT_RS18885 0.23 -4.2 4,244,228 - BPHYT_RS18885 0.23 -1.7 4,244,228 - BPHYT_RS18885 0.23 -4.7 4,244,547 - BPHYT_RS18885 0.67 -5.0 4,244,547 - BPHYT_RS18885 0.67 -4.1 4,244,726 + -3.0 4,244,727 - -4.8 4,244,933 + BPHYT_RS18890 0.32 -5.2 4,244,933 + BPHYT_RS18890 0.32 -3.1 4,244,934 - BPHYT_RS18890 0.32 -3.5
Or see this region's nucleotide sequence