Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18820 and BPHYT_RS18825 overlap by 1 nucleotides BPHYT_RS18825 and BPHYT_RS18830 are separated by 49 nucleotides BPHYT_RS18830 and BPHYT_RS18835 are separated by 37 nucleotides BPHYT_RS18835 and BPHYT_RS18840 are separated by 97 nucleotides
BPHYT_RS18820: BPHYT_RS18820 - flagellar biosynthesis protein FliO, at 4,230,821 to 4,231,336
_RS18820
BPHYT_RS18825: BPHYT_RS18825 - flagellar biosynthesis protein FliP, at 4,231,336 to 4,232,169
_RS18825
BPHYT_RS18830: BPHYT_RS18830 - flagellar biosynthesis protein FliQ, at 4,232,219 to 4,232,488
_RS18830
BPHYT_RS18835: BPHYT_RS18835 - flagellar biosynthesis protein FliR, at 4,232,526 to 4,233,308
_RS18835
BPHYT_RS18840: BPHYT_RS18840 - flagellar hook-associated protein FlgL, at 4,233,406 to 4,234,629
_RS18840
Position (kb) 4232
4233 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.547 kb on - strand, within BPHYT_RS18825 at 4231.759 kb on + strand, within BPHYT_RS18825 at 4231.760 kb on - strand, within BPHYT_RS18825 at 4231.761 kb on + strand, within BPHYT_RS18825 at 4231.762 kb on - strand, within BPHYT_RS18825 at 4231.762 kb on - strand, within BPHYT_RS18825 at 4231.772 kb on - strand, within BPHYT_RS18825 at 4232.083 kb on - strand, within BPHYT_RS18825 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.911 kb on - strand, within BPHYT_RS18835 at 4233.060 kb on - strand, within BPHYT_RS18835 at 4233.234 kb on - strand at 4233.234 kb on - strand at 4233.269 kb on + strand at 4233.306 kb on + strand at 4233.395 kb on + strand at 4233.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,231,284 + BPHYT_RS18820 0.90 +0.5 4,231,284 + BPHYT_RS18820 0.90 +0.2 4,231,547 - BPHYT_RS18825 0.25 -0.9 4,231,759 + BPHYT_RS18825 0.51 -3.3 4,231,760 - BPHYT_RS18825 0.51 -0.6 4,231,761 + BPHYT_RS18825 0.51 -1.6 4,231,762 - BPHYT_RS18825 0.51 -3.2 4,231,762 - BPHYT_RS18825 0.51 -0.9 4,231,772 - BPHYT_RS18825 0.52 -1.1 4,232,083 - BPHYT_RS18825 0.90 -3.2 4,232,261 + BPHYT_RS18830 0.16 -4.9 4,232,261 + BPHYT_RS18830 0.16 -2.0 4,232,261 + BPHYT_RS18830 0.16 -3.5 4,232,261 + BPHYT_RS18830 0.16 -3.9 4,232,261 + BPHYT_RS18830 0.16 -2.0 4,232,261 + BPHYT_RS18830 0.16 -5.0 4,232,261 + BPHYT_RS18830 0.16 -3.7 4,232,261 + -0.2 4,232,261 + BPHYT_RS18830 0.16 +0.4 4,232,262 - BPHYT_RS18830 0.16 -2.1 4,232,262 - BPHYT_RS18830 0.16 -1.2 4,232,262 - BPHYT_RS18830 0.16 -4.9 4,232,262 - BPHYT_RS18830 0.16 -1.9 4,232,262 - BPHYT_RS18830 0.16 -4.3 4,232,262 - BPHYT_RS18830 0.16 -4.1 4,232,262 - BPHYT_RS18830 0.16 -4.4 4,232,262 - BPHYT_RS18830 0.16 -3.0 4,232,262 - BPHYT_RS18830 0.16 -4.9 4,232,911 - BPHYT_RS18835 0.49 -0.9 4,233,060 - BPHYT_RS18835 0.68 -3.0 4,233,234 - +0.4 4,233,234 - -3.9 4,233,269 + -0.2 4,233,306 + -0.9 4,233,395 + +0.3 4,233,405 - -0.8
Or see this region's nucleotide sequence