Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18760 and BPHYT_RS18765 overlap by 4 nucleotides BPHYT_RS18765 and BPHYT_RS18770 are separated by 216 nucleotides BPHYT_RS18770 and BPHYT_RS18775 are separated by 235 nucleotides
BPHYT_RS18760: BPHYT_RS18760 - flagellar biosynthesis protein FliT, at 4,219,551 to 4,219,877
_RS18760
BPHYT_RS18765: BPHYT_RS18765 - flagellar biosynthesis protein FliS, at 4,219,874 to 4,220,308
_RS18765
BPHYT_RS18770: BPHYT_RS18770 - flagellar hook-basal body protein FliE, at 4,220,525 to 4,220,860
_RS18770
BPHYT_RS18775: BPHYT_RS18775 - flagellar M-ring protein FliF, at 4,221,096 to 4,222,907
_RS18775
Position (kb) 4220
4221 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 4219.685 kb on - strand, within BPHYT_RS18760 at 4219.685 kb on - strand, within BPHYT_RS18760 at 4219.759 kb on + strand, within BPHYT_RS18760 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.036 kb on - strand, within BPHYT_RS18765 at 4220.036 kb on - strand, within BPHYT_RS18765 at 4220.038 kb on - strand, within BPHYT_RS18765 at 4220.038 kb on - strand, within BPHYT_RS18765 at 4220.275 kb on - strand at 4220.275 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.420 kb on + strand at 4220.421 kb on - strand at 4220.440 kb on + strand at 4220.441 kb on - strand at 4220.553 kb on + strand at 4220.554 kb on - strand at 4220.779 kb on + strand, within BPHYT_RS18770 at 4220.932 kb on - strand at 4220.950 kb on + strand at 4220.970 kb on - strand at 4220.970 kb on - strand at 4220.970 kb on - strand at 4221.363 kb on + strand, within BPHYT_RS18775 at 4221.364 kb on - strand, within BPHYT_RS18775 at 4221.364 kb on - strand, within BPHYT_RS18775 at 4221.442 kb on - strand, within BPHYT_RS18775 at 4221.442 kb on - strand, within BPHYT_RS18775 at 4221.442 kb on - strand, within BPHYT_RS18775 at 4221.442 kb on - strand, within BPHYT_RS18775 at 4221.606 kb on + strand, within BPHYT_RS18775 at 4221.606 kb on + strand, within BPHYT_RS18775 at 4221.606 kb on + strand, within BPHYT_RS18775 at 4221.607 kb on - strand, within BPHYT_RS18775 at 4221.800 kb on + strand, within BPHYT_RS18775 at 4221.800 kb on + strand, within BPHYT_RS18775 at 4221.800 kb on + strand, within BPHYT_RS18775 at 4221.800 kb on + strand, within BPHYT_RS18775 at 4221.800 kb on + strand, within BPHYT_RS18775 at 4221.801 kb on - strand, within BPHYT_RS18775 at 4221.801 kb on - strand, within BPHYT_RS18775
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,219,685 - BPHYT_RS18760 0.41 -0.6 4,219,685 - BPHYT_RS18760 0.41 -1.4 4,219,759 + BPHYT_RS18760 0.64 -0.4 4,220,030 - BPHYT_RS18765 0.36 -6.4 4,220,030 - BPHYT_RS18765 0.36 -3.6 4,220,030 - BPHYT_RS18765 0.36 +1.1 4,220,030 - BPHYT_RS18765 0.36 -3.3 4,220,030 - BPHYT_RS18765 0.36 -3.1 4,220,032 - BPHYT_RS18765 0.36 -3.6 4,220,032 - BPHYT_RS18765 0.36 -4.1 4,220,032 - BPHYT_RS18765 0.36 -1.4 4,220,036 - BPHYT_RS18765 0.37 -5.8 4,220,036 - BPHYT_RS18765 0.37 -2.9 4,220,038 - BPHYT_RS18765 0.38 -1.6 4,220,038 - BPHYT_RS18765 0.38 -1.2 4,220,275 - -0.9 4,220,275 - -4.3 4,220,388 - +0.4 4,220,388 - -0.3 4,220,388 - -1.3 4,220,388 - -1.0 4,220,420 + +0.4 4,220,421 - +0.9 4,220,440 + -1.1 4,220,441 - +0.8 4,220,553 + -5.7 4,220,554 - -2.2 4,220,779 + BPHYT_RS18770 0.76 -0.6 4,220,932 - -1.3 4,220,950 + -5.6 4,220,970 - -1.5 4,220,970 - -2.7 4,220,970 - -6.2 4,221,363 + BPHYT_RS18775 0.15 -2.7 4,221,364 - BPHYT_RS18775 0.15 -1.4 4,221,364 - BPHYT_RS18775 0.15 -4.6 4,221,442 - BPHYT_RS18775 0.19 -4.2 4,221,442 - BPHYT_RS18775 0.19 -1.9 4,221,442 - BPHYT_RS18775 0.19 -4.0 4,221,442 - BPHYT_RS18775 0.19 -6.2 4,221,606 + BPHYT_RS18775 0.28 -1.8 4,221,606 + BPHYT_RS18775 0.28 -3.0 4,221,606 + BPHYT_RS18775 0.28 -4.2 4,221,607 - BPHYT_RS18775 0.28 -0.6 4,221,800 + BPHYT_RS18775 0.39 -3.1 4,221,800 + BPHYT_RS18775 0.39 -2.8 4,221,800 + BPHYT_RS18775 0.39 -4.4 4,221,800 + BPHYT_RS18775 0.39 -1.4 4,221,800 + BPHYT_RS18775 0.39 -2.0 4,221,801 - BPHYT_RS18775 0.39 -0.2 4,221,801 - BPHYT_RS18775 0.39 -3.9
Or see this region's nucleotide sequence