Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17900 and BPHYT_RS17905 overlap by 4 nucleotides BPHYT_RS17905 and BPHYT_RS17910 overlap by 4 nucleotides BPHYT_RS17910 and BPHYT_RS17915 are separated by 254 nucleotides
BPHYT_RS17900: BPHYT_RS17900 - hypothetical protein, at 4,042,182 to 4,043,114
_RS17900
BPHYT_RS17905: BPHYT_RS17905 - fimbrial assembly family protein, at 4,043,111 to 4,043,830
_RS17905
BPHYT_RS17910: BPHYT_RS17910 - hypothetical protein, at 4,043,827 to 4,044,819
_RS17910
BPHYT_RS17915: BPHYT_RS17915 - penicillin-binding protein 1A, at 4,045,074 to 4,047,473
_RS17915
Position (kb) 4043
4044
4045 Strain fitness (log2 ratio) -2
-1
0
1 at 4043.108 kb on + strand at 4043.414 kb on + strand, within BPHYT_RS17905 at 4043.459 kb on + strand, within BPHYT_RS17905 at 4043.459 kb on + strand, within BPHYT_RS17905 at 4043.466 kb on - strand, within BPHYT_RS17905 at 4043.959 kb on + strand, within BPHYT_RS17910 at 4043.960 kb on - strand, within BPHYT_RS17910 at 4044.144 kb on - strand, within BPHYT_RS17910 at 4044.144 kb on - strand, within BPHYT_RS17910 at 4044.176 kb on + strand, within BPHYT_RS17910 at 4044.176 kb on + strand, within BPHYT_RS17910 at 4044.176 kb on + strand, within BPHYT_RS17910 at 4044.176 kb on + strand, within BPHYT_RS17910 at 4044.176 kb on + strand, within BPHYT_RS17910 at 4044.176 kb on + strand, within BPHYT_RS17910 at 4044.177 kb on - strand, within BPHYT_RS17910 at 4044.177 kb on - strand, within BPHYT_RS17910 at 4044.350 kb on + strand, within BPHYT_RS17910 at 4044.351 kb on - strand, within BPHYT_RS17910 at 4044.351 kb on - strand, within BPHYT_RS17910 at 4044.351 kb on - strand, within BPHYT_RS17910 at 4044.365 kb on + strand, within BPHYT_RS17910 at 4044.884 kb on + strand at 4044.960 kb on + strand at 4045.010 kb on + strand at 4045.011 kb on - strand at 4045.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,043,108 + +0.2 4,043,414 + BPHYT_RS17905 0.42 -0.6 4,043,459 + BPHYT_RS17905 0.48 -1.0 4,043,459 + BPHYT_RS17905 0.48 -0.7 4,043,466 - BPHYT_RS17905 0.49 -1.3 4,043,959 + BPHYT_RS17910 0.13 +0.3 4,043,960 - BPHYT_RS17910 0.13 -1.7 4,044,144 - BPHYT_RS17910 0.32 -0.9 4,044,144 - BPHYT_RS17910 0.32 -1.6 4,044,176 + BPHYT_RS17910 0.35 -0.6 4,044,176 + BPHYT_RS17910 0.35 -0.9 4,044,176 + BPHYT_RS17910 0.35 +1.2 4,044,176 + BPHYT_RS17910 0.35 -0.2 4,044,176 + BPHYT_RS17910 0.35 -0.1 4,044,176 + BPHYT_RS17910 0.35 -1.1 4,044,177 - BPHYT_RS17910 0.35 -0.2 4,044,177 - BPHYT_RS17910 0.35 -0.1 4,044,350 + BPHYT_RS17910 0.53 -0.8 4,044,351 - BPHYT_RS17910 0.53 -0.3 4,044,351 - BPHYT_RS17910 0.53 -0.7 4,044,351 - BPHYT_RS17910 0.53 -1.7 4,044,365 + BPHYT_RS17910 0.54 -1.8 4,044,884 + -1.3 4,044,960 + -0.2 4,045,010 + -0.8 4,045,011 - +1.2 4,045,071 + -0.6
Or see this region's nucleotide sequence