Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10810 and BPHYT_RS10815 are separated by 45 nucleotides BPHYT_RS10815 and BPHYT_RS10820 are separated by 3 nucleotides BPHYT_RS10820 and BPHYT_RS10825 overlap by 8 nucleotides BPHYT_RS10825 and BPHYT_RS10830 are separated by 23 nucleotides
BPHYT_RS10810: BPHYT_RS10810 - glucose-methanol-choline oxidoreductase, at 2,457,055 to 2,458,788
_RS10810
BPHYT_RS10815: BPHYT_RS10815 - crotonase, at 2,458,834 to 2,459,640
_RS10815
BPHYT_RS10820: BPHYT_RS10820 - 4-chlorobenzoate--CoA ligase, at 2,459,644 to 2,461,221
_RS10820
BPHYT_RS10825: BPHYT_RS10825 - (2Fe-2S)-binding protein, at 2,461,214 to 2,461,744
_RS10825
BPHYT_RS10830: BPHYT_RS10830 - carbon monoxide dehydrogenase, at 2,461,768 to 2,462,589
_RS10830
Position (kb)
2459
2460
2461
2462 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2458.773 kb on - strand at 2458.804 kb on - strand at 2458.804 kb on - strand at 2458.919 kb on + strand, within BPHYT_RS10815 at 2458.919 kb on + strand, within BPHYT_RS10815 at 2459.756 kb on - strand at 2459.774 kb on + strand at 2460.017 kb on + strand, within BPHYT_RS10820 at 2460.018 kb on - strand, within BPHYT_RS10820 at 2460.288 kb on - strand, within BPHYT_RS10820 at 2460.306 kb on - strand, within BPHYT_RS10820 at 2460.409 kb on - strand, within BPHYT_RS10820 at 2460.449 kb on + strand, within BPHYT_RS10820 at 2460.593 kb on + strand, within BPHYT_RS10820 at 2460.725 kb on + strand, within BPHYT_RS10820 at 2460.725 kb on + strand, within BPHYT_RS10820 at 2461.134 kb on - strand at 2461.736 kb on + strand at 2461.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,458,773 - +1.3 2,458,804 - +0.3 2,458,804 - +0.1 2,458,919 + BPHYT_RS10815 0.11 -0.9 2,458,919 + BPHYT_RS10815 0.11 +1.7 2,459,756 - +0.7 2,459,774 + -0.9 2,460,017 + BPHYT_RS10820 0.24 -2.1 2,460,018 - BPHYT_RS10820 0.24 +2.6 2,460,288 - BPHYT_RS10820 0.41 +1.3 2,460,306 - BPHYT_RS10820 0.42 -0.3 2,460,409 - BPHYT_RS10820 0.48 +0.5 2,460,449 + BPHYT_RS10820 0.51 -1.2 2,460,593 + BPHYT_RS10820 0.60 -0.9 2,460,725 + BPHYT_RS10820 0.69 -1.1 2,460,725 + BPHYT_RS10820 0.69 +2.0 2,461,134 - -0.3 2,461,736 + +0.8 2,461,737 - -1.2
Or see this region's nucleotide sequence