Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10800

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10790 and BPHYT_RS10795 are separated by 46 nucleotidesBPHYT_RS10795 and BPHYT_RS10800 are separated by 176 nucleotidesBPHYT_RS10800 and BPHYT_RS10805 overlap by 4 nucleotides BPHYT_RS10790: BPHYT_RS10790 - C4-dicarboxylate ABC transporter substrate-binding protein, at 2,452,334 to 2,453,686 _RS10790 BPHYT_RS10795: BPHYT_RS10795 - ProQ activator of osmoprotectant transporter ProP, at 2,453,733 to 2,454,488 _RS10795 BPHYT_RS10800: BPHYT_RS10800 - LysR family transcriptional regulator, at 2,454,665 to 2,455,588 _RS10800 BPHYT_RS10805: BPHYT_RS10805 - MFS transporter, at 2,455,585 to 2,456,961 _RS10805 Position (kb) 2454 2455 2456Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2453.704 kb on - strandat 2454.521 kb on - strandat 2454.581 kb on + strandat 2454.743 kb on - strandat 2454.857 kb on + strandat 2454.866 kb on + strand, within BPHYT_RS10800at 2454.866 kb on + strand, within BPHYT_RS10800at 2454.867 kb on - strand, within BPHYT_RS10800at 2454.867 kb on - strand, within BPHYT_RS10800at 2455.183 kb on - strand, within BPHYT_RS10800at 2455.586 kb on + strandat 2455.899 kb on + strand, within BPHYT_RS10805at 2455.899 kb on + strand, within BPHYT_RS10805at 2456.130 kb on - strand, within BPHYT_RS10805at 2456.423 kb on + strand, within BPHYT_RS10805at 2456.496 kb on - strand, within BPHYT_RS10805

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,453,704 - -2.8
2,454,521 - +0.8
2,454,581 + -0.1
2,454,743 - -0.4
2,454,857 + +2.9
2,454,866 + BPHYT_RS10800 0.22 +0.2
2,454,866 + BPHYT_RS10800 0.22 -0.3
2,454,867 - BPHYT_RS10800 0.22 -0.3
2,454,867 - BPHYT_RS10800 0.22 -1.7
2,455,183 - BPHYT_RS10800 0.56 +0.3
2,455,586 + -0.3
2,455,899 + BPHYT_RS10805 0.23 -0.2
2,455,899 + BPHYT_RS10805 0.23 -0.3
2,456,130 - BPHYT_RS10805 0.40 -1.1
2,456,423 + BPHYT_RS10805 0.61 -0.6
2,456,496 - BPHYT_RS10805 0.66 -0.8

Or see this region's nucleotide sequence