Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10170

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10165 and BPHYT_RS10170 are separated by 88 nucleotidesBPHYT_RS10170 and BPHYT_RS10175 are separated by 9 nucleotidesBPHYT_RS10175 and BPHYT_RS10180 are separated by 95 nucleotides BPHYT_RS10165: BPHYT_RS10165 - transcriptional regulator, at 2,316,027 to 2,316,470 _RS10165 BPHYT_RS10170: BPHYT_RS10170 - oxidoreductase, at 2,316,559 to 2,317,419 _RS10170 BPHYT_RS10175: BPHYT_RS10175 - membrane protein, at 2,317,429 to 2,317,836 _RS10175 BPHYT_RS10180: BPHYT_RS10180 - NADPH:quinone oxidoreductase, at 2,317,932 to 2,318,933 _RS10180 Position (kb) 2316 2317 2318Strain fitness (log2 ratio) -1 0 1 2at 2315.640 kb on - strandat 2315.640 kb on - strandat 2315.660 kb on - strandat 2315.727 kb on + strandat 2315.728 kb on - strandat 2315.728 kb on - strandat 2316.232 kb on + strand, within BPHYT_RS10165at 2316.276 kb on + strand, within BPHYT_RS10165at 2316.652 kb on + strand, within BPHYT_RS10170at 2316.654 kb on + strand, within BPHYT_RS10170at 2316.685 kb on + strand, within BPHYT_RS10170at 2316.687 kb on + strand, within BPHYT_RS10170at 2316.919 kb on + strand, within BPHYT_RS10170at 2316.919 kb on + strand, within BPHYT_RS10170at 2316.919 kb on + strand, within BPHYT_RS10170at 2317.070 kb on + strand, within BPHYT_RS10170at 2317.070 kb on + strand, within BPHYT_RS10170at 2317.070 kb on + strand, within BPHYT_RS10170at 2317.096 kb on + strand, within BPHYT_RS10170at 2317.526 kb on + strand, within BPHYT_RS10175at 2317.585 kb on + strand, within BPHYT_RS10175at 2318.021 kb on - strandat 2318.193 kb on + strand, within BPHYT_RS10180

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,315,640 - +0.4
2,315,640 - -0.3
2,315,660 - +1.3
2,315,727 + -0.3
2,315,728 - -0.2
2,315,728 - -0.2
2,316,232 + BPHYT_RS10165 0.46 +1.9
2,316,276 + BPHYT_RS10165 0.56 +0.7
2,316,652 + BPHYT_RS10170 0.11 -0.4
2,316,654 + BPHYT_RS10170 0.11 +0.6
2,316,685 + BPHYT_RS10170 0.15 -1.6
2,316,687 + BPHYT_RS10170 0.15 +1.0
2,316,919 + BPHYT_RS10170 0.42 +0.1
2,316,919 + BPHYT_RS10170 0.42 +1.1
2,316,919 + BPHYT_RS10170 0.42 +0.4
2,317,070 + BPHYT_RS10170 0.59 +0.5
2,317,070 + BPHYT_RS10170 0.59 -0.4
2,317,070 + BPHYT_RS10170 0.59 +0.9
2,317,096 + BPHYT_RS10170 0.62 -0.1
2,317,526 + BPHYT_RS10175 0.24 +0.6
2,317,585 + BPHYT_RS10175 0.38 -0.3
2,318,021 - -0.0
2,318,193 + BPHYT_RS10180 0.26 -1.6

Or see this region's nucleotide sequence