Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08250 and BPHYT_RS08255 are separated by 266 nucleotides BPHYT_RS08255 and BPHYT_RS08260 are separated by 120 nucleotides BPHYT_RS08260 and BPHYT_RS08265 overlap by 4 nucleotides
BPHYT_RS08250: BPHYT_RS08250 - hypothetical protein, at 1,884,195 to 1,884,662
_RS08250
BPHYT_RS08255: BPHYT_RS08255 - cresol methylhydroxylase, at 1,884,929 to 1,886,506
_RS08255
BPHYT_RS08260: BPHYT_RS08260 - hypothetical protein, at 1,886,627 to 1,887,085
_RS08260
BPHYT_RS08265: BPHYT_RS08265 - membrane protein, at 1,887,082 to 1,888,002
_RS08265
Position (kb)
1884
1885
1886
1887 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1884.108 kb on - strand at 1884.179 kb on + strand at 1884.441 kb on + strand, within BPHYT_RS08250 at 1884.442 kb on - strand, within BPHYT_RS08250 at 1884.459 kb on + strand, within BPHYT_RS08250 at 1884.549 kb on + strand, within BPHYT_RS08250 at 1885.290 kb on - strand, within BPHYT_RS08255 at 1885.316 kb on + strand, within BPHYT_RS08255 at 1885.329 kb on - strand, within BPHYT_RS08255 at 1885.353 kb on - strand, within BPHYT_RS08255 at 1885.405 kb on + strand, within BPHYT_RS08255 at 1885.429 kb on + strand, within BPHYT_RS08255 at 1885.595 kb on + strand, within BPHYT_RS08255 at 1885.802 kb on + strand, within BPHYT_RS08255 at 1886.186 kb on + strand, within BPHYT_RS08255 at 1886.348 kb on + strand, within BPHYT_RS08255 at 1886.348 kb on + strand, within BPHYT_RS08255 at 1886.349 kb on - strand at 1886.349 kb on - strand at 1886.349 kb on - strand at 1886.350 kb on + strand at 1886.372 kb on + strand at 1886.373 kb on - strand at 1887.083 kb on + strand at 1887.092 kb on + strand at 1887.093 kb on - strand at 1887.154 kb on + strand at 1887.188 kb on + strand, within BPHYT_RS08265 at 1887.189 kb on - strand, within BPHYT_RS08265 at 1887.251 kb on + strand, within BPHYT_RS08265 at 1887.380 kb on + strand, within BPHYT_RS08265 at 1887.395 kb on + strand, within BPHYT_RS08265 at 1887.396 kb on - strand, within BPHYT_RS08265 at 1887.396 kb on - strand, within BPHYT_RS08265 at 1887.504 kb on - strand, within BPHYT_RS08265
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,884,108 - -2.0 1,884,179 + -0.5 1,884,441 + BPHYT_RS08250 0.53 -0.3 1,884,442 - BPHYT_RS08250 0.53 -0.2 1,884,459 + BPHYT_RS08250 0.56 +0.1 1,884,549 + BPHYT_RS08250 0.76 +0.8 1,885,290 - BPHYT_RS08255 0.23 -0.2 1,885,316 + BPHYT_RS08255 0.25 -0.7 1,885,329 - BPHYT_RS08255 0.25 -0.3 1,885,353 - BPHYT_RS08255 0.27 -0.6 1,885,405 + BPHYT_RS08255 0.30 -0.9 1,885,429 + BPHYT_RS08255 0.32 -0.1 1,885,595 + BPHYT_RS08255 0.42 -0.6 1,885,802 + BPHYT_RS08255 0.55 -0.3 1,886,186 + BPHYT_RS08255 0.80 -0.6 1,886,348 + BPHYT_RS08255 0.90 -0.5 1,886,348 + BPHYT_RS08255 0.90 -0.2 1,886,349 - -0.3 1,886,349 - -0.3 1,886,349 - -0.3 1,886,350 + -0.1 1,886,372 + -2.8 1,886,373 - +0.8 1,887,083 + +0.1 1,887,092 + -0.4 1,887,093 - -0.2 1,887,154 + -0.3 1,887,188 + BPHYT_RS08265 0.12 +1.1 1,887,189 - BPHYT_RS08265 0.12 +1.3 1,887,251 + BPHYT_RS08265 0.18 -0.6 1,887,380 + BPHYT_RS08265 0.32 -0.5 1,887,395 + BPHYT_RS08265 0.34 +0.7 1,887,396 - BPHYT_RS08265 0.34 +1.7 1,887,396 - BPHYT_RS08265 0.34 +0.7 1,887,504 - BPHYT_RS08265 0.46 -0.3
Or see this region's nucleotide sequence