Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08255

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08250 and BPHYT_RS08255 are separated by 266 nucleotidesBPHYT_RS08255 and BPHYT_RS08260 are separated by 120 nucleotidesBPHYT_RS08260 and BPHYT_RS08265 overlap by 4 nucleotides BPHYT_RS08250: BPHYT_RS08250 - hypothetical protein, at 1,884,195 to 1,884,662 _RS08250 BPHYT_RS08255: BPHYT_RS08255 - cresol methylhydroxylase, at 1,884,929 to 1,886,506 _RS08255 BPHYT_RS08260: BPHYT_RS08260 - hypothetical protein, at 1,886,627 to 1,887,085 _RS08260 BPHYT_RS08265: BPHYT_RS08265 - membrane protein, at 1,887,082 to 1,888,002 _RS08265 Position (kb) 1884 1885 1886 1887Strain fitness (log2 ratio) -3 -2 -1 0 1at 1884.108 kb on - strandat 1884.179 kb on + strandat 1884.441 kb on + strand, within BPHYT_RS08250at 1884.442 kb on - strand, within BPHYT_RS08250at 1884.459 kb on + strand, within BPHYT_RS08250at 1884.549 kb on + strand, within BPHYT_RS08250at 1885.290 kb on - strand, within BPHYT_RS08255at 1885.316 kb on + strand, within BPHYT_RS08255at 1885.329 kb on - strand, within BPHYT_RS08255at 1885.353 kb on - strand, within BPHYT_RS08255at 1885.405 kb on + strand, within BPHYT_RS08255at 1885.429 kb on + strand, within BPHYT_RS08255at 1885.595 kb on + strand, within BPHYT_RS08255at 1885.802 kb on + strand, within BPHYT_RS08255at 1886.186 kb on + strand, within BPHYT_RS08255at 1886.348 kb on + strand, within BPHYT_RS08255at 1886.348 kb on + strand, within BPHYT_RS08255at 1886.349 kb on - strandat 1886.349 kb on - strandat 1886.349 kb on - strandat 1886.350 kb on + strandat 1886.372 kb on + strandat 1886.373 kb on - strandat 1887.083 kb on + strandat 1887.092 kb on + strandat 1887.093 kb on - strandat 1887.154 kb on + strandat 1887.188 kb on + strand, within BPHYT_RS08265at 1887.189 kb on - strand, within BPHYT_RS08265at 1887.251 kb on + strand, within BPHYT_RS08265at 1887.380 kb on + strand, within BPHYT_RS08265at 1887.395 kb on + strand, within BPHYT_RS08265at 1887.396 kb on - strand, within BPHYT_RS08265at 1887.396 kb on - strand, within BPHYT_RS08265at 1887.504 kb on - strand, within BPHYT_RS08265

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,884,108 - -2.0
1,884,179 + -0.5
1,884,441 + BPHYT_RS08250 0.53 -0.3
1,884,442 - BPHYT_RS08250 0.53 -0.2
1,884,459 + BPHYT_RS08250 0.56 +0.1
1,884,549 + BPHYT_RS08250 0.76 +0.8
1,885,290 - BPHYT_RS08255 0.23 -0.2
1,885,316 + BPHYT_RS08255 0.25 -0.7
1,885,329 - BPHYT_RS08255 0.25 -0.3
1,885,353 - BPHYT_RS08255 0.27 -0.6
1,885,405 + BPHYT_RS08255 0.30 -0.9
1,885,429 + BPHYT_RS08255 0.32 -0.1
1,885,595 + BPHYT_RS08255 0.42 -0.6
1,885,802 + BPHYT_RS08255 0.55 -0.3
1,886,186 + BPHYT_RS08255 0.80 -0.6
1,886,348 + BPHYT_RS08255 0.90 -0.5
1,886,348 + BPHYT_RS08255 0.90 -0.2
1,886,349 - -0.3
1,886,349 - -0.3
1,886,349 - -0.3
1,886,350 + -0.1
1,886,372 + -2.8
1,886,373 - +0.8
1,887,083 + +0.1
1,887,092 + -0.4
1,887,093 - -0.2
1,887,154 + -0.3
1,887,188 + BPHYT_RS08265 0.12 +1.1
1,887,189 - BPHYT_RS08265 0.12 +1.3
1,887,251 + BPHYT_RS08265 0.18 -0.6
1,887,380 + BPHYT_RS08265 0.32 -0.5
1,887,395 + BPHYT_RS08265 0.34 +0.7
1,887,396 - BPHYT_RS08265 0.34 +1.7
1,887,396 - BPHYT_RS08265 0.34 +0.7
1,887,504 - BPHYT_RS08265 0.46 -0.3

Or see this region's nucleotide sequence