Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08070 and BPHYT_RS08075 are separated by 215 nucleotides BPHYT_RS08075 and BPHYT_RS08080 are separated by 94 nucleotides BPHYT_RS08080 and BPHYT_RS08085 are separated by 129 nucleotides
BPHYT_RS08070: BPHYT_RS08070 - translation initiation factor IF-2, at 1,839,804 to 1,842,764
_RS08070
BPHYT_RS08075: BPHYT_RS08075 - ribosome-binding factor A, at 1,842,980 to 1,843,345
_RS08075
BPHYT_RS08080: BPHYT_RS08080 - tRNA pseudouridine synthase B, at 1,843,440 to 1,844,375
_RS08080
BPHYT_RS08085: BPHYT_RS08085 - DSBA oxidoreductase, at 1,844,505 to 1,846,067
_RS08085
Position (kb)
1843
1844
1845 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1842.755 kb on + strand at 1842.755 kb on + strand at 1843.412 kb on - strand at 1843.412 kb on - strand at 1843.421 kb on - strand at 1843.554 kb on + strand, within BPHYT_RS08080 at 1843.667 kb on + strand, within BPHYT_RS08080 at 1843.751 kb on - strand, within BPHYT_RS08080 at 1843.824 kb on + strand, within BPHYT_RS08080 at 1843.825 kb on - strand, within BPHYT_RS08080 at 1843.857 kb on + strand, within BPHYT_RS08080 at 1843.951 kb on + strand, within BPHYT_RS08080 at 1843.983 kb on + strand, within BPHYT_RS08080 at 1843.983 kb on + strand, within BPHYT_RS08080 at 1843.983 kb on + strand, within BPHYT_RS08080 at 1843.984 kb on - strand, within BPHYT_RS08080 at 1843.984 kb on - strand, within BPHYT_RS08080 at 1843.984 kb on - strand, within BPHYT_RS08080 at 1843.984 kb on - strand, within BPHYT_RS08080 at 1843.984 kb on - strand, within BPHYT_RS08080 at 1843.984 kb on - strand, within BPHYT_RS08080 at 1844.393 kb on + strand at 1844.394 kb on - strand at 1844.394 kb on - strand at 1844.468 kb on - strand at 1844.469 kb on + strand at 1844.474 kb on + strand at 1844.474 kb on + strand at 1844.475 kb on - strand at 1844.737 kb on + strand, within BPHYT_RS08085 at 1844.737 kb on + strand, within BPHYT_RS08085 at 1844.897 kb on + strand, within BPHYT_RS08085 at 1844.898 kb on - strand, within BPHYT_RS08085 at 1844.898 kb on - strand, within BPHYT_RS08085 at 1844.898 kb on - strand, within BPHYT_RS08085 at 1844.898 kb on - strand, within BPHYT_RS08085 at 1844.952 kb on - strand, within BPHYT_RS08085 at 1845.038 kb on + strand, within BPHYT_RS08085 at 1845.044 kb on + strand, within BPHYT_RS08085 at 1845.148 kb on + strand, within BPHYT_RS08085 at 1845.264 kb on + strand, within BPHYT_RS08085 at 1845.265 kb on - strand, within BPHYT_RS08085
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,842,755 + -1.7 1,842,755 + +0.3 1,843,412 - +0.8 1,843,412 - -0.9 1,843,421 - +1.2 1,843,554 + BPHYT_RS08080 0.12 -0.3 1,843,667 + BPHYT_RS08080 0.24 +0.2 1,843,751 - BPHYT_RS08080 0.33 +2.9 1,843,824 + BPHYT_RS08080 0.41 +0.4 1,843,825 - BPHYT_RS08080 0.41 -0.2 1,843,857 + BPHYT_RS08080 0.45 +0.5 1,843,951 + BPHYT_RS08080 0.55 -2.2 1,843,983 + BPHYT_RS08080 0.58 +0.9 1,843,983 + BPHYT_RS08080 0.58 +0.3 1,843,983 + BPHYT_RS08080 0.58 +0.6 1,843,984 - BPHYT_RS08080 0.58 -0.5 1,843,984 - BPHYT_RS08080 0.58 +0.9 1,843,984 - BPHYT_RS08080 0.58 -1.6 1,843,984 - BPHYT_RS08080 0.58 -2.0 1,843,984 - BPHYT_RS08080 0.58 +0.6 1,843,984 - BPHYT_RS08080 0.58 +0.2 1,844,393 + +0.3 1,844,394 - -0.4 1,844,394 - -0.8 1,844,468 - +2.0 1,844,469 + -0.7 1,844,474 + +0.1 1,844,474 + -0.2 1,844,475 - +0.9 1,844,737 + BPHYT_RS08085 0.15 -0.2 1,844,737 + BPHYT_RS08085 0.15 +1.6 1,844,897 + BPHYT_RS08085 0.25 +1.0 1,844,898 - BPHYT_RS08085 0.25 -0.6 1,844,898 - BPHYT_RS08085 0.25 +0.1 1,844,898 - BPHYT_RS08085 0.25 +0.4 1,844,898 - BPHYT_RS08085 0.25 -0.2 1,844,952 - BPHYT_RS08085 0.29 +1.6 1,845,038 + BPHYT_RS08085 0.34 +0.2 1,845,044 + BPHYT_RS08085 0.34 -1.2 1,845,148 + BPHYT_RS08085 0.41 +0.1 1,845,264 + BPHYT_RS08085 0.49 +0.3 1,845,265 - BPHYT_RS08085 0.49 -0.3
Or see this region's nucleotide sequence