Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03460 and BPHYT_RS03465 overlap by 4 nucleotides BPHYT_RS03465 and BPHYT_RS03470 are separated by 787 nucleotides
BPHYT_RS03460: BPHYT_RS03460 - mammalian cell entry protein, at 779,249 to 780,907
_RS03460
BPHYT_RS03465: BPHYT_RS03465 - hypothetical protein, at 780,904 to 781,650
_RS03465
BPHYT_RS03470: BPHYT_RS03470 - LysR family transcriptional regulator, at 782,438 to 783,397
_RS03470
Position (kb)
780
781
782 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 780.285 kb on - strand, within BPHYT_RS03460 at 780.473 kb on + strand, within BPHYT_RS03460 at 780.473 kb on + strand, within BPHYT_RS03460 at 780.474 kb on - strand, within BPHYT_RS03460 at 780.487 kb on + strand, within BPHYT_RS03460 at 781.022 kb on - strand, within BPHYT_RS03465 at 781.023 kb on + strand, within BPHYT_RS03465 at 781.038 kb on + strand, within BPHYT_RS03465 at 781.276 kb on + strand, within BPHYT_RS03465 at 781.276 kb on + strand, within BPHYT_RS03465 at 781.309 kb on + strand, within BPHYT_RS03465 at 781.309 kb on + strand, within BPHYT_RS03465 at 781.309 kb on + strand, within BPHYT_RS03465 at 781.443 kb on + strand, within BPHYT_RS03465 at 781.528 kb on - strand, within BPHYT_RS03465 at 781.707 kb on - strand at 781.890 kb on + strand at 782.082 kb on - strand at 782.082 kb on - strand at 782.212 kb on - strand at 782.212 kb on - strand at 782.258 kb on + strand at 782.258 kb on + strand at 782.289 kb on + strand at 782.388 kb on + strand at 782.394 kb on + strand at 782.397 kb on - strand at 782.400 kb on + strand at 782.400 kb on + strand at 782.459 kb on + strand at 782.459 kb on + strand at 782.460 kb on - strand at 782.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 780,285 - BPHYT_RS03460 0.62 -0.0 780,473 + BPHYT_RS03460 0.74 -2.0 780,473 + BPHYT_RS03460 0.74 +1.3 780,474 - BPHYT_RS03460 0.74 -0.6 780,487 + BPHYT_RS03460 0.75 +3.1 781,022 - BPHYT_RS03465 0.16 -1.3 781,023 + BPHYT_RS03465 0.16 +0.1 781,038 + BPHYT_RS03465 0.18 -0.1 781,276 + BPHYT_RS03465 0.50 +0.5 781,276 + BPHYT_RS03465 0.50 -0.1 781,309 + BPHYT_RS03465 0.54 -0.3 781,309 + BPHYT_RS03465 0.54 -0.8 781,309 + BPHYT_RS03465 0.54 -0.6 781,443 + BPHYT_RS03465 0.72 -2.3 781,528 - BPHYT_RS03465 0.84 -1.0 781,707 - -0.6 781,890 + -0.9 782,082 - -0.1 782,082 - +0.1 782,212 - +0.2 782,212 - +0.6 782,258 + -0.2 782,258 + +1.8 782,289 + -0.9 782,388 + +0.5 782,394 + +0.7 782,397 - -0.0 782,400 + +1.0 782,400 + -1.4 782,459 + -0.3 782,459 + +0.3 782,460 - +0.8 782,460 - +0.2
Or see this region's nucleotide sequence