Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03275 and BPHYT_RS03280 are separated by 71 nucleotides BPHYT_RS03280 and BPHYT_RS03285 are separated by 3 nucleotides BPHYT_RS03285 and BPHYT_RS03290 are separated by 223 nucleotides
BPHYT_RS03275: BPHYT_RS03275 - metal ABC transporter permease, at 736,144 to 738,033
_RS03275
BPHYT_RS03280: BPHYT_RS03280 - mannose-1-phosphate guanylyltransferase, at 738,105 to 738,833
_RS03280
BPHYT_RS03285: BPHYT_RS03285 - aminoglycoside phosphotransferase, at 738,837 to 739,928
_RS03285
BPHYT_RS03290: BPHYT_RS03290 - LPS-assembly protein LptD, at 740,152 to 742,518
_RS03290
Position (kb) 738
739
740 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 738.107 kb on - strand at 738.107 kb on - strand at 738.191 kb on - strand, within BPHYT_RS03280 at 738.481 kb on + strand, within BPHYT_RS03280 at 738.847 kb on + strand at 738.913 kb on + strand at 738.914 kb on - strand at 738.967 kb on + strand, within BPHYT_RS03285 at 738.967 kb on + strand, within BPHYT_RS03285 at 738.968 kb on - strand, within BPHYT_RS03285 at 738.968 kb on - strand, within BPHYT_RS03285 at 739.114 kb on + strand, within BPHYT_RS03285 at 739.117 kb on + strand, within BPHYT_RS03285 at 739.190 kb on - strand, within BPHYT_RS03285 at 739.190 kb on - strand, within BPHYT_RS03285 at 739.204 kb on + strand, within BPHYT_RS03285 at 739.205 kb on - strand, within BPHYT_RS03285 at 739.968 kb on + strand at 739.968 kb on + strand at 739.969 kb on - strand at 739.969 kb on - strand at 739.970 kb on + strand at 739.970 kb on + strand at 739.970 kb on + strand at 739.970 kb on + strand at 740.055 kb on + strand at 740.055 kb on + strand at 740.059 kb on + strand at 740.149 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 738,107 - -0.2 738,107 - -0.1 738,191 - BPHYT_RS03280 0.12 -3.1 738,481 + BPHYT_RS03280 0.52 -0.4 738,847 + -1.1 738,913 + -0.5 738,914 - +0.2 738,967 + BPHYT_RS03285 0.12 -0.7 738,967 + BPHYT_RS03285 0.12 -1.1 738,968 - BPHYT_RS03285 0.12 +1.9 738,968 - BPHYT_RS03285 0.12 -1.2 739,114 + BPHYT_RS03285 0.25 +0.2 739,117 + BPHYT_RS03285 0.26 -1.0 739,190 - BPHYT_RS03285 0.32 -0.6 739,190 - BPHYT_RS03285 0.32 -1.5 739,204 + BPHYT_RS03285 0.34 -1.5 739,205 - BPHYT_RS03285 0.34 +0.4 739,968 + -2.3 739,968 + -1.4 739,969 - -1.4 739,969 - -1.3 739,970 + -1.2 739,970 + +0.4 739,970 + +0.2 739,970 + -0.5 740,055 + -0.1 740,055 + -0.4 740,059 + -0.1 740,149 + -0.3
Or see this region's nucleotide sequence