Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02285 and BPHYT_RS02290 are separated by 61 nucleotides BPHYT_RS02290 and BPHYT_RS02295 are separated by 442 nucleotides BPHYT_RS02295 and BPHYT_RS02300 are separated by 142 nucleotides
BPHYT_RS02285: BPHYT_RS02285 - cell division protein, at 523,185 to 524,009
_RS02285
BPHYT_RS02290: BPHYT_RS02290 - arginine--tRNA ligase, at 524,071 to 525,861
_RS02290
BPHYT_RS02295: BPHYT_RS02295 - hypothetical protein, at 526,304 to 526,630
_RS02295
BPHYT_RS02300: BPHYT_RS02300 - acid shock protein, at 526,773 to 526,982
_RS02300
Position (kb) 524
525
526 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 523.136 kb on - strand at 523.171 kb on + strand at 523.171 kb on + strand at 523.172 kb on - strand at 523.172 kb on - strand at 523.172 kb on - strand at 523.175 kb on + strand at 523.176 kb on - strand at 523.176 kb on - strand at 523.187 kb on - strand at 523.187 kb on - strand at 523.294 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.298 kb on - strand, within BPHYT_RS02285 at 523.879 kb on + strand, within BPHYT_RS02285 at 523.879 kb on + strand, within BPHYT_RS02285 at 524.072 kb on + strand at 524.072 kb on + strand at 524.072 kb on + strand at 524.073 kb on - strand at 524.073 kb on - strand at 526.075 kb on - strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.176 kb on - strand at 526.176 kb on - strand at 526.313 kb on - strand at 526.313 kb on - strand at 526.313 kb on - strand at 526.367 kb on + strand, within BPHYT_RS02295 at 526.367 kb on + strand, within BPHYT_RS02295 at 526.367 kb on + strand, within BPHYT_RS02295 at 526.368 kb on - strand, within BPHYT_RS02295 at 526.719 kb on + strand at 526.720 kb on - strand at 526.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 523,136 - +1.5 523,171 + -0.4 523,171 + +0.2 523,172 - +0.5 523,172 - +0.2 523,172 - +0.8 523,175 + -1.4 523,176 - -0.5 523,176 - +0.9 523,187 - -0.4 523,187 - -0.2 523,294 + BPHYT_RS02285 0.13 -1.9 523,297 + BPHYT_RS02285 0.14 +1.0 523,297 + BPHYT_RS02285 0.14 -2.4 523,297 + BPHYT_RS02285 0.14 -2.3 523,298 - BPHYT_RS02285 0.14 -2.6 523,879 + BPHYT_RS02285 0.84 -2.6 523,879 + BPHYT_RS02285 0.84 -3.0 524,072 + -2.1 524,072 + -1.2 524,072 + -1.1 524,073 - -2.4 524,073 - -0.1 526,075 - +0.1 526,172 + -0.2 526,172 + +0.5 526,172 + -2.3 526,172 + -1.1 526,172 + +0.1 526,173 - -0.8 526,173 - -0.3 526,173 - +1.1 526,173 - -0.3 526,175 + +1.1 526,175 + -0.3 526,175 + -0.1 526,175 + +0.0 526,175 + +0.5 526,175 + +0.6 526,176 - -0.9 526,176 - +0.0 526,313 - +0.1 526,313 - +1.1 526,313 - +0.1 526,367 + BPHYT_RS02295 0.19 +0.2 526,367 + BPHYT_RS02295 0.19 +0.1 526,367 + BPHYT_RS02295 0.19 +0.2 526,368 - BPHYT_RS02295 0.20 +2.0 526,719 + +0.1 526,720 - -0.7 526,775 - +1.1
Or see this region's nucleotide sequence