Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02180 and BPHYT_RS02185 are separated by 280 nucleotides BPHYT_RS02185 and BPHYT_RS02190 are separated by 260 nucleotides BPHYT_RS02190 and BPHYT_RS02195 are separated by 181 nucleotides
BPHYT_RS02180: BPHYT_RS02180 - hypothetical protein, at 501,848 to 502,372
_RS02180
BPHYT_RS02185: BPHYT_RS02185 - hypothetical protein, at 502,653 to 503,165
_RS02185
BPHYT_RS02190: BPHYT_RS02190 - hypothetical protein, at 503,426 to 503,971
_RS02190
BPHYT_RS02195: BPHYT_RS02195 - hypothetical protein, at 504,153 to 504,689
_RS02195
Position (kb) 502
503
504 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 501.667 kb on + strand at 501.667 kb on + strand at 501.746 kb on - strand at 502.019 kb on - strand, within BPHYT_RS02180 at 502.178 kb on + strand, within BPHYT_RS02180 at 502.261 kb on + strand, within BPHYT_RS02180 at 502.410 kb on - strand at 502.516 kb on + strand at 502.680 kb on - strand at 502.847 kb on + strand, within BPHYT_RS02185 at 502.848 kb on - strand, within BPHYT_RS02185 at 503.100 kb on + strand, within BPHYT_RS02185 at 503.178 kb on + strand at 503.187 kb on + strand at 503.188 kb on - strand at 503.278 kb on - strand at 503.437 kb on - strand at 503.504 kb on + strand, within BPHYT_RS02190 at 503.505 kb on - strand, within BPHYT_RS02190 at 503.545 kb on + strand, within BPHYT_RS02190 at 503.545 kb on + strand, within BPHYT_RS02190 at 503.545 kb on + strand, within BPHYT_RS02190 at 503.546 kb on - strand, within BPHYT_RS02190 at 503.761 kb on - strand, within BPHYT_RS02190 at 503.774 kb on + strand, within BPHYT_RS02190 at 503.805 kb on - strand, within BPHYT_RS02190 at 503.993 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 501,667 + -0.5 501,667 + -0.5 501,746 - +0.0 502,019 - BPHYT_RS02180 0.33 -1.4 502,178 + BPHYT_RS02180 0.63 -0.5 502,261 + BPHYT_RS02180 0.79 -0.6 502,410 - +0.3 502,516 + -1.6 502,680 - -0.9 502,847 + BPHYT_RS02185 0.38 -1.0 502,848 - BPHYT_RS02185 0.38 -0.0 503,100 + BPHYT_RS02185 0.87 -1.1 503,178 + -0.6 503,187 + -1.8 503,188 - -0.1 503,278 - -3.5 503,437 - -1.5 503,504 + BPHYT_RS02190 0.14 +1.9 503,505 - BPHYT_RS02190 0.14 +0.7 503,545 + BPHYT_RS02190 0.22 -0.2 503,545 + BPHYT_RS02190 0.22 +1.5 503,545 + BPHYT_RS02190 0.22 +0.5 503,546 - BPHYT_RS02190 0.22 -0.2 503,761 - BPHYT_RS02190 0.61 -0.0 503,774 + BPHYT_RS02190 0.64 +0.2 503,805 - BPHYT_RS02190 0.69 -5.6 503,993 + -2.5
Or see this region's nucleotide sequence