Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02155

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02150 and BPHYT_RS02155 are separated by 33 nucleotidesBPHYT_RS02155 and BPHYT_RS02160 overlap by 4 nucleotides BPHYT_RS02150: BPHYT_RS02150 - biotin synthase, at 492,275 to 493,360 _RS02150 BPHYT_RS02155: BPHYT_RS02155 - dithiobiotin synthetase, at 493,394 to 494,137 _RS02155 BPHYT_RS02160: BPHYT_RS02160 - 8-amino-7-oxononanoate synthase, at 494,134 to 495,318 _RS02160 Position (kb) 493 494 495Strain fitness (log2 ratio) -3 -2 -1 0 1at 492.778 kb on - strand, within BPHYT_RS02150at 492.837 kb on + strand, within BPHYT_RS02150at 492.926 kb on + strand, within BPHYT_RS02150at 493.374 kb on - strandat 493.383 kb on + strandat 493.384 kb on - strandat 493.384 kb on - strandat 493.395 kb on + strandat 493.521 kb on + strand, within BPHYT_RS02155at 493.522 kb on - strand, within BPHYT_RS02155at 493.825 kb on + strand, within BPHYT_RS02155at 493.825 kb on + strand, within BPHYT_RS02155at 493.825 kb on + strand, within BPHYT_RS02155at 493.897 kb on - strand, within BPHYT_RS02155at 493.964 kb on - strand, within BPHYT_RS02155at 493.970 kb on + strand, within BPHYT_RS02155at 494.106 kb on - strandat 494.516 kb on + strand, within BPHYT_RS02160at 494.859 kb on - strand, within BPHYT_RS02160at 494.859 kb on - strand, within BPHYT_RS02160at 494.982 kb on - strand, within BPHYT_RS02160

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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492,778 - BPHYT_RS02150 0.46 -1.1
492,837 + BPHYT_RS02150 0.52 +0.1
492,926 + BPHYT_RS02150 0.60 -0.6
493,374 - +0.3
493,383 + -0.8
493,384 - -2.9
493,384 - +0.6
493,395 + +0.3
493,521 + BPHYT_RS02155 0.17 -1.3
493,522 - BPHYT_RS02155 0.17 -0.9
493,825 + BPHYT_RS02155 0.58 -1.4
493,825 + BPHYT_RS02155 0.58 -1.2
493,825 + BPHYT_RS02155 0.58 -1.4
493,897 - BPHYT_RS02155 0.68 -1.8
493,964 - BPHYT_RS02155 0.77 -1.1
493,970 + BPHYT_RS02155 0.77 -0.6
494,106 - -0.6
494,516 + BPHYT_RS02160 0.32 -1.7
494,859 - BPHYT_RS02160 0.61 +0.2
494,859 - BPHYT_RS02160 0.61 +0.8
494,982 - BPHYT_RS02160 0.72 +1.1

Or see this region's nucleotide sequence