Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01935 and BPHYT_RS01940 are separated by 129 nucleotides BPHYT_RS01940 and BPHYT_RS01945 are separated by 79 nucleotides BPHYT_RS01945 and BPHYT_RS01950 are separated by 216 nucleotides
BPHYT_RS01935: BPHYT_RS01935 - lipoprotein, at 439,977 to 440,609
_RS01935
BPHYT_RS01940: BPHYT_RS01940 - 16S rRNA methyltransferase, at 440,739 to 441,620
_RS01940
BPHYT_RS01945: BPHYT_RS01945 - hypothetical protein, at 441,700 to 442,122
_RS01945
BPHYT_RS01950: BPHYT_RS01950 - phosphoheptose isomerase, at 442,339 to 442,926
_RS01950
Position (kb) 440
441
442 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 439.741 kb on + strand at 440.006 kb on + strand at 440.007 kb on - strand at 440.007 kb on - strand at 440.007 kb on - strand at 440.091 kb on - strand, within BPHYT_RS01935 at 440.325 kb on + strand, within BPHYT_RS01935 at 440.325 kb on + strand, within BPHYT_RS01935 at 440.357 kb on + strand, within BPHYT_RS01935 at 440.357 kb on + strand, within BPHYT_RS01935 at 440.357 kb on + strand, within BPHYT_RS01935 at 440.357 kb on + strand, within BPHYT_RS01935 at 440.357 kb on + strand, within BPHYT_RS01935 at 440.357 kb on + strand at 440.357 kb on + strand, within BPHYT_RS01935 at 440.358 kb on - strand at 440.358 kb on - strand, within BPHYT_RS01935 at 440.358 kb on - strand, within BPHYT_RS01935 at 440.376 kb on + strand, within BPHYT_RS01935 at 440.377 kb on - strand, within BPHYT_RS01935 at 440.424 kb on - strand, within BPHYT_RS01935 at 440.443 kb on - strand, within BPHYT_RS01935 at 440.565 kb on - strand at 440.565 kb on - strand at 440.648 kb on - strand at 440.779 kb on + strand at 440.779 kb on + strand at 440.779 kb on + strand at 440.862 kb on + strand, within BPHYT_RS01940 at 440.919 kb on + strand, within BPHYT_RS01940 at 440.920 kb on - strand, within BPHYT_RS01940 at 441.006 kb on + strand, within BPHYT_RS01940 at 441.299 kb on + strand, within BPHYT_RS01940 at 441.343 kb on + strand, within BPHYT_RS01940 at 441.343 kb on + strand, within BPHYT_RS01940 at 441.344 kb on - strand, within BPHYT_RS01940 at 441.344 kb on - strand, within BPHYT_RS01940 at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.566 kb on - strand at 441.566 kb on - strand at 441.567 kb on + strand at 441.568 kb on - strand at 441.568 kb on - strand at 441.578 kb on + strand at 441.981 kb on + strand, within BPHYT_RS01945 at 441.981 kb on + strand, within BPHYT_RS01945 at 441.982 kb on - strand, within BPHYT_RS01945 at 441.982 kb on - strand, within BPHYT_RS01945 at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.214 kb on - strand at 442.329 kb on + strand at 442.619 kb on - strand, within BPHYT_RS01950
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 439,741 + +0.3 440,006 + -0.3 440,007 - -2.4 440,007 - +0.3 440,007 - +1.5 440,091 - BPHYT_RS01935 0.18 +0.1 440,325 + BPHYT_RS01935 0.55 +0.4 440,325 + BPHYT_RS01935 0.55 +0.1 440,357 + BPHYT_RS01935 0.60 +1.6 440,357 + BPHYT_RS01935 0.60 +1.1 440,357 + BPHYT_RS01935 0.60 +0.6 440,357 + BPHYT_RS01935 0.60 -0.8 440,357 + BPHYT_RS01935 0.60 +0.2 440,357 + +1.4 440,357 + BPHYT_RS01935 0.60 +0.2 440,358 - -0.6 440,358 - BPHYT_RS01935 0.60 +0.2 440,358 - BPHYT_RS01935 0.60 +0.7 440,376 + BPHYT_RS01935 0.63 -0.5 440,377 - BPHYT_RS01935 0.63 +0.9 440,424 - BPHYT_RS01935 0.71 +0.4 440,443 - BPHYT_RS01935 0.74 -0.2 440,565 - +0.7 440,565 - -0.4 440,648 - -2.4 440,779 + -1.3 440,779 + +0.2 440,779 + -1.2 440,862 + BPHYT_RS01940 0.14 -1.4 440,919 + BPHYT_RS01940 0.20 +0.2 440,920 - BPHYT_RS01940 0.21 -0.2 441,006 + BPHYT_RS01940 0.30 -0.3 441,299 + BPHYT_RS01940 0.63 +0.4 441,343 + BPHYT_RS01940 0.68 -1.2 441,343 + BPHYT_RS01940 0.68 -0.3 441,344 - BPHYT_RS01940 0.69 +0.3 441,344 - BPHYT_RS01940 0.69 +0.1 441,565 + -1.9 441,565 + -0.9 441,565 + -0.7 441,565 + -0.7 441,565 + -0.7 441,565 + -0.8 441,566 - -0.3 441,566 - -3.0 441,567 + -1.6 441,568 - +0.4 441,568 - -1.4 441,578 + -0.0 441,981 + BPHYT_RS01945 0.66 +1.0 441,981 + BPHYT_RS01945 0.66 -0.1 441,982 - BPHYT_RS01945 0.67 +0.5 441,982 - BPHYT_RS01945 0.67 -0.9 442,213 + -0.6 442,213 + -0.4 442,213 + -0.6 442,213 + -0.4 442,213 + +0.4 442,214 - -0.9 442,329 + -0.4 442,619 - BPHYT_RS01950 0.48 -2.1
Or see this region's nucleotide sequence